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Citations for Merck :
5651 - 5700 of 5854 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fractions corresponding to CVB5 were combined and concentrated to 4.5 mg ml-1 using Amicon Ultra centrifugal filter units with 100 kDa MWCO (Merck Millipore).
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris- buffered salione containing 0.1% Tween-20 (v/v) (TBST) fore 60 mins at RT and incubated overnight with primary antibody against REST (1:500) (07-579, Merck), H3K9me2 (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Genetics 2024Quote: ... Cells were washed with water thrice and released into YPD media containing 15 µg mL-1 nocodazole (Cat. No. M1404, Merck) for 90 min ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 2.5 µg mL-1 α-factor grown at 25°C (Cat. No. T6901, Merck). After 90 min ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was pelleted by centrifugation at 3,000 g for 10 min at 4°C and the pellet was resuspended in 100 μl of 1% SDS lysis buffer (1% SDS in 50 mM Tris-HCl (pH=8.0) and supplemented with 1X protease inhibitor cocktail (from 100X; Merck, P8849), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated to ∼ 10 mg/mL (as judged by the extinction coefficient χ410= 213 mM-1cm-1) using Amicon 50K MWCO centrifugal concentrators (Merck). Dimeric NmqNOR was immediately used to make grids following SEC ...
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bioRxiv - Microbiology 2024Quote: ... Peak fractions were concentrated to 1.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary incubation was completed with a 1:40 dilution of Goat anti-Rabbit IgG 10 nm gold conjugate for a further hour (Merck).
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bioRxiv - Plant Biology 2024Quote: ... AtPFA-DSP1 and AtNUDT17 were resuspended in buffer A (50 mM Tris pH 7.5, 500 mM NaCl, 1 mM DTT, Dnase I and cOmplete™ protease inhibitor cocktail [Merck]), MpPFA-DSP1 and MpNUDT1 were resuspended in buffer B (50 mM K2HPO4/KH2PO4 pH 7.8 ...
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bioRxiv - Immunology 2024Quote: Splenocytes were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: DNA encoding the TBD domain of human ASK1 (residues 88–267) was ligated into pRSFDuet-1 (Merck KGaA, Darmstadt, Germany) using BamHI and NotI sites ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were fixed in 1% glutaraldehyde in 1x PBS for 30 min and stained with filtered 10% Giemsa (Merck) in 1x PBS for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... All supernatant was removed using a 1 ml syringe and the beads were resuspended in 30 μl 2X SDS Sample Buffer (Merck Millipore). The samples were incubated at 98°C for 5 minutes before electrophoresis.
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bioRxiv - Developmental Biology 2021Quote: ... Lymphocytes were isolated from the spleen and incubated in a tissue culture tube at a cell concentration of 1 × 106/ml in RPMI1640 (Nacalai Tesque) containing lipopolysaccharide (10 μg/ml, Merck KGaA), concanavalin A (3 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin pellet was digested in 100 μl of nuclease-free water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 minutes at 37°C in a thermomixer at 1,400 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The solution consisted of collagenase type I (1 mg/ml) prepared in Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12, Sigma Aldrich/Merck, Poznan, Poland). The tissue fragments were digested for 40 minutes in CO2 incubator at 37 °C with periodic vortexing ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed using ice-cold RIPA buffer containing 1% of protease and phosphatase inhibitor cocktail (Sigma Aldrich/Merck, Poznan, Polska). The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Bioengineering 2019Quote: ... The hPBMCs were washed, re-suspended in freezing medium (RPMI-1640 supplemented with 20 % FBS, 1 % P/S, and 10 % Dimethyl sulfoxide (Merck Millipore)) ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunofluorescence using primary polyclonal antibodies that label neurons (NeuN, mouse; 1: 1000, Millipore MAB377, Merck Millipore), reactive astrocytes (GFAP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed and stained according to the procedure described above using primary antibodies against NESTIN (Merck, ABD69, 1:300 dilution) and TUBB3 (Santa Cruz ...
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bioRxiv - Biophysics 2021Quote: ... and concentrated to >1.5 mg/mL.The DNA and antibody solutions were cross-reacted at a 10:1 molar ratio overnight and excess DNA was filtered through a 100 kDa Amicon spin column (UFC510096, Merck Milipore). The antibody-DNA solution was stored at 4°C.
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bioRxiv - Genomics 2019Quote: ... heads of 10 ISO-1 females were chopped in 500 µL of ice-cold Otto 1 Buffer (0.1 mol/L citric acid, 0.5% Tween20 (Merck KGaA, Darmstadt, Germany)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Plant Biology 2021Quote: ... The eluate containing the nanodiscs was concentrated to 1 ml with the use of an Amicon Ultra-15 10K device (Merck Millipore) and then mixed with 10 ml of translation buffer containing 30 mM HEPES-KOH (pH 7.8 ...
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bioRxiv - Biochemistry 2021Quote: ... truncatula leaf tissue was ground in liquid nitrogen and extracted three times in 1 ml of 80% HPLC-grade methanol (Merck, Germany). Each time the mixture was sonicated for 15 min at room temperature and centrifuged for 10 min at 15,000 ×g ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μL of the extract-reaction mixture was added to 1 cm2 P81 Phosphocellulose paper squares (MERCK MILLIPORE, Billerica, MA, USA). The papers were immersed directly in 1% (w/v ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis seeds were surface sterilized with 1 mL 70% (v/v) ethanol containing 0.05% (v/v) Tween 20 (Merck, Darmstadt, Germany) by shaking on a table incubator at 1400 rpm for 3 min ...
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bioRxiv - Biophysics 2021Quote: ... The DNA and antibody solutions were cross-reacted at a 10:1 molar ratio overnight and excess DNA filtered through a 100 kDa Amicon spin column (UFC510096, Merck Milipore). The antibody-DNA solution was stored at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were dissolved in 1 ml of PBS and nuclei were stained with propidium iodide (PI) (Merck Millipore, Burlington, MA, USA) and analyzed using a flow cytometer (Guava PCA) ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet obtained after the initial low-speed centrifugation was incubated in ice-cold IP buffer containing 1% NP-40 and 25-50 U of Benzonase (Merck, 70746) for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 50% confluent cells were plated and allowed to adhere for at least 24 hours before treatment with 1 μM 4OH-TAM (Merck, H7904) or with identical volume of EtOH for the indicated time ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... pooled and concentrated to roughly 0.5 – 1 mL by ultrafiltration spin units Amicon® Ultra–15 10 kDa MWCO (Merck-Millipore). Buffer was exchanged against storage buffer (40 mM Hepes (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour with a species-appropriate HRP-conjugated secondary antibody (diluted 1:5000 in TBST) before bands were visualised using Immobilon Forte Western HRP Substrate (Merck Millipore). Primary antibodies used were mouse anti-αSMA (Dako ...
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bioRxiv - Cell Biology 2019Quote: ... Total β1-integrin was detected by immunoblotting the membrane again with clone N29 anti-β1-integrin antibody (Merck, MAB2252, 1:1000) and appropriate secondary antibody and analysis on the Odyssey (LI-COR ...