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Citations for Merck :
4951 - 5000 of 5322 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 25 mM Tris-HCl, 25 mM NaCl, 1 mM EDTA, pH 7.6, supplemented with 1X protease inhibitor cocktail i.e. PIC, Merck #4693132001). Further ...
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bioRxiv - Microbiology 2023Quote: ... A 40 mg of lyophilized fecal sample was extracted with 1 ml of HPLC grade methanol (Merck, Mumbai, India) and kept in a shaker overnight at 1200 rpm ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated (12-well plate) on glass coverslips coated with poly-L-lysin-hydrochloride (1 mg/ml, Merck). After 2h ...
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bioRxiv - Physiology 2023Quote: ... Mice were injected intravenously (i.v.) two consecutive days with 1 mg of biotin-X-NHS Ester (BXN) (Millipore, Merck) per mouse dissolved in 200 μl of saline buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Fabs were concentrated up to >1 mg/ml using 10 k cut-off filters (Amicon Ultra-0.5 10 k; Merck). Fabs were conjugated with Alexa Fluor 488 (Sulfodichlorophenol Ester ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were then pelleted by centrifugation and lysed in a nuclei lysis buffer (50mM Tris HCl pH8.1, 150mM NaCl, 10mM EDTA, 1% SDS, Complete protease inhibitor cocktail (Merck)) for 20min ...
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bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Ammonium persulphate ((NH4)2S2O8) 98.0% (APS) and glutaraldehyde (OHC(CH2)3CHO) (Grade 1, 25% in water) were obtained from Merck, Germany ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Immunology 2023Quote: ... benthamiana was validated by immunoblot analysis using a polyclonal anti-luciferase primary antibody (Merck; used in 1:1000 dilution).
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bioRxiv - Developmental Biology 2023Quote: ... mature pollen was germinated on the surface of solid PGM (18% sucrose, 0.01% H3BO3, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 (Merck), pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... was performed at room temp for 1h together with DNA staining by DAPI (1 µg/ml, Merck Life science). Cells were washed an additional 3x and imaged on an LSM880 confocal microscope (Zeiss) ...
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bioRxiv - Biochemistry 2023Quote: ... coli pellet in 7.5 mL of TBS- N (Tris buffered saline with 0.1% NP-40) and supplemented with 1 x protease inhibitor cocktail tablet (Merck). Cells were sonicated for four rounds of one minute on ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tyrosine-hydroxylase (TH) was detected with the primary antibody anti-TH in rabbit (1:600, EMD Millipore - Merck, USA). After washing in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was further concentrated to volumes <1 mL using 100kDa MWCO Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). Tricorn 10/300 chromatography columns (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 4 times for 5 min with 1 x PBS/Tween20 0.1 % (v/v) and the HRP conjugated secondary antibodies (A5420 and A4416, Merck) were added 1:5000 (goat ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred on PVDF membranes and rabbit polyclonal antibodies were used at a 1:500 dilution (anti-Pic and anti-SepA) or a 1:250 dilution (anti-SigA) in 1X TBS supplemented with 0,5% of Tween 20 (Merck) and 3% BSA (Euromedex) ...
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bioRxiv - Biophysics 2023Quote: ... 5.5 µL of fibril sample was mixed with 0.5 µL of 1 mg/mL pronase protease (53702, Merck-Millipore) followed by adsorbing onto carbon-coated copper grids ...
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bioRxiv - Bioengineering 2024Quote: ... the THP-1 macrophages were detached from the MadSurface by incubating in 1mL/well Accutase (Merck Life Science NV) in the incubator for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... The cytoskeleton was stained using a 1:40 dilution of Alexa Fluor 488 conjugated phalloidin (Merck Life Science NV) in PBS and nuclei were stained using a 1:1000 dilution of DAPI (Merck Life Science NV) ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed by resuspension in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.2% IGEPAL® [Merck]) and pelleted again in the magnetic rack ...
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bioRxiv - Neuroscience 2024Quote: ... 10 worms from each RNAi treatment group were collected and cleaned in H2O before depositing in 1 μL of lysis buffer (0.25 mM EDTA (Merck), 5mM Tris pH 8.0 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibodies against γH2AX (the same antibody used for wester blotting) and 53BP1 (1:1000, MAB3802 Merck). Cells were washed and incubated with secondary anti-rabbit-Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8) and permeabilized and blocked with 1X TBS supplemented with 1% Triton-X100 (MERCK; Cat #9036-19-5) and 6% FBS (Biological Industries ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained residue was derivatized with N-trimethylsilylimidazole/ trimethylchlorosilane reagent (TMSI:TMCS, 99:1, v/v; 50uL; both from Merck) and pyridine (50uL ...
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bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
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bioRxiv - Plant Biology 2024Quote: ... the buffer was replaced with complex IV staining solution [1 mg/mL DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Merck D5637), 1 mg/mL cytochrome c (Merck C7752)] in complex IV buffer) ...
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bioRxiv - Microbiology 2024Quote: ... Mouse mAb anti-HA (clone HA-7) and mouse anti-tubulin (clone B5-1-12) were purchased from Merck. Mouse mAb anti-V5 (SV5-PK1 ...
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bioRxiv - Microbiology 2024Quote: “Swim” agar plates were made by pouring 0.3% LB agar into petri dishes with a 1-50 numbered grid sticker on them (Merck). Ciprofloxacin was added from a stock solution to each plate to a final concentration of either 0.015 μg mL-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells were transferred to a hypoxia chamber (InvivO2, Baker Ruskinn) set at 1% O2 and treated with increasing concentrations of PT2399 (Peloton Therapeutics/Merck) for 48 hours 22 ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample (0.5-1 ml) was transferred to Amicon Ultra 0.5 ml Centrifugal Filters (MWCO=30 kDa, Merck, cat# UFC503096) and further concentrated to a final volume of 200 μl.
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred into polyvinylidene difluoride (PVDF) membrane in 1× Transfer buffer (Tris-base/Glycine/Methanol, Sigma-Aldrich/Merck, Poznan, Poland) using the Mini Trans-Blot® system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... was diluted (1:1000) in 500 μl 0.1% PBST with 0.2% normal donkey serum (S30-100ML, Merck Millipore, Hertfordshire, UK) and sections were protected from light and incubated at RT with agitation for 2 hours ...