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Citations for Biotek :
301 - 350 of 455 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... SP Plant DNA Kit (Omega BioTek, Norcross, GA) (Pyne et al. ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counterstained with DAPI and imaged at 10X using a Lionheart FX automated imaging system (BioTek). Infected cells (FFU ...
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bioRxiv - Microbiology 2023Quote: ... and the growth curve was monitored on a Cytation™ 5 plate reader system (BioTek Instruments Inc) for 72 hours ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... genomic DNA was extracted from HEK293T cells using Geno Plus (TM) Genomic DNA Extraction Miniprep System (Viogene-BioTek). Then ...
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bioRxiv - Bioengineering 2021Quote: ... a mini bioreactor system (48 BioReactor, 2mag AG, Munich, Germany) and a Synergy MX microwell plate reader (BioTek Instruments GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... we compared the proliferation of control and LMNA knockdown cells using the BioSpa Live Cell Analysis system (BioTek) and performed Ki67 immunofluorescence staining (Santa Cruz Biotechnology).
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bioRxiv - Cell Biology 2024Quote: ... the plate containing the follicles was transferred to a live-cell imaging system (Agilent BioTek Cytation 5, USA) to capture images at 6-minute intervals during ovulation ...
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bioRxiv - Molecular Biology 2021Quote: The dual-luciferase assay was performed using a Cytation™ 5 plate reader with a dual-injector system (BioTek). For each sample ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Plant Biology 2019Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were isolated using a DNA miniprep kit (Omega BioTek) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Immunology 2023Quote: ... and E.Z.N.A.® HP Total RNA kit (Omega Biotek, U.S.). RNA samples were sequenced using the Illumina HiSeq platform to generate 50-bp paired-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... and using Total RNA Kit I (Omega Biotek, R6834-02) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were washed once with PBS and stored in PBS prior to automated high content imaging with a 4x objective on a Lionheart imaging system (Biotek). Viability was measured by counting cell nuclei using Gen5 software (Biotek ...
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bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
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bioRxiv - Developmental Biology 2019Quote: ... Firefly and Renilla bioluminescence readings were obtained from 20 μl of cleared lysate using the Synergy 2 plate reader and Gen 5 analysis software system (BioTek). Firefly bioluminescence was normalized to renilla levels ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with Hoechst 33342 and counted for GFP+ and total cells with Cytation 5 high-content live-cell imaging system (Biotek) using a 4x objective.
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bioRxiv - Neuroscience 2022Quote: ... Screening plates were handled in a semi-automated system with VIAFLO 96/384 handheld electronic pipette (INTEGRA) and Multi-Mode Dispenser MultiFlo FX (BioTek). Screening assay was performed with H1/H1 line diku treated and differentiated into neurons with standard day 3 media change with minus AO media ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... XFe data normalization was performed by in situ nuclear staining and in situ cell counting using the imaging and normalization system (BioTek Cytation).
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bioRxiv - Cancer Biology 2020Quote: Normal prostate epithelial cells and prostate cancer cells were seeded at optimal density in 384-well plates using an automated dispensing system (BioTek EL406). EZH2 inhibitors (GSK126 or EPZ-6438 ...
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bioRxiv - Microbiology 2020Quote: ... Microplates are read at 450 nm with 650 nm subtraction with an OD450 nm cut-off of 0.071(Biotek Cytation system). mAb were quantified using Prism software ...
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bioRxiv - Developmental Biology 2023Quote: ... Changes in fluorescence intensity of active cells were recorded using high-throughput live cell imaging system for 10 minutes (BioTek-lionheart FX, BioTek, USA). Fluorescent image stacks were processed by Fiji software 69 and plotted using Prism 8 software (GraphPad).
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using an Omega total RNA extraction kit (Omega Biotek) (column-based system ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL, cat. #50-76-00) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody data were analyzed by nonlinear regression (One site total binding ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL, cat. #50-76-00) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody data were analyzed by nonlinear regression (One site total binding ...
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bioRxiv - Biochemistry 2021Quote: ... Daily cell number was determined by addition of 20 μl of AqueousOne Solution and measurement of absorbance at 490 nm using a Cytation 5 imaging system (BioTek, Winooski, VT). Fold change was calculated by dividing by absorbance measurements taken at day zero.
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bioRxiv - Biochemistry 2021Quote: ... The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the media of lower channel was collected and the FITC-dextran concentration was determined by FITC fluorescence intensity using a microplate reader system (BioTek Synergy H1) at 490 nm (Ex ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 3M sulfuric acid 5-10 minutes after OPD addition and absorbance values were measured at a wavelength of 492nm on a spectrophotometer (Biotek Cytation5 system). Absorbance values were normalized by subtracting uncoated wells from antigen-coated wells at the corresponding serum dilution ...
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bioRxiv - Cell Biology 2021Quote: ... four days after transfection and luciferase activity was measured using the Nano-Glo® Dual-Luciferase® Reporter Assay System using the Synergy Neo2 multi-mode reader (BioTek). Nanoluciferase activity was normalized to that of the firefly luciferase.
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bioRxiv - Biochemistry 2023Quote: ... Relative activities of the firefly and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... staining and washing steps (2x, wash volume 50 µl) were performed using automated liquid handling systems (405 plate washer, BioTek– Tempest, Formulatrix).
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bioRxiv - Neuroscience 2024Quote: ... was measured in cell culture medium collected from the top and bottom chamber of the Transwell system (three technical replicates per Transwell) using a fluorescent plate reader (Biotek Synergy H4) and clearance volume calculated as previously described (37,38).
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).