Labshake search
Citations for Biotek :
201 - 250 of 455 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, Winooski, VT). Data was collected using Gen5™ Data Analysis Software (BioTek Instruments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Both luciferase activity and fluorescence intensity measurements were performed on a SynergyTM H1 hybrid multi-mode reader (BioTek, Bad Friedrichshall, Germany) assessing at least five independent experiments in technical duplicates.
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bioRxiv - Microbiology 2022Quote: ... The optical density of plates was evaluated at 450 nm using a microplate reader (BioTek Synergy H1 Hybrid Multi-Mode Reader). EC50 values were estimated using the ATT BioQuest® - EC50 calculator.
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bioRxiv - Cancer Biology 2019Quote: ... The cells were then put in the incubator for 45 min and readings were obtained using a Synergy H4 Hybrid Reader (BioTek, USA) using excitation/emission wavelengths of 492 nm/525 nm ...
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bioRxiv - Microbiology 2019Quote: ... as substrate which is dephosphorylated by the ALP enzyme forming p-nitrophenol which was measured spectrophotometrically at 405 nm in Synergy H1 Hybrid Reader (BioTek, USA) (43) ...
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bioRxiv - Biochemistry 2020Quote: ... The spectra were recorded using Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek and were averaged by 3 scans.
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bioRxiv - Microbiology 2023Quote: ... and the fluorescence (Ex: 485/20, Em: 528/20) and absorbance (OD600) were measured in a Synergy Hybrid HTX Microplate Reader (BioTek Instruments). One quarter of the unstained wells were processed for cell viability and one quarter were processed for crystal violet staining to assess total biofilm formation ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence (excitation 360 nm, emission 410 nm) was measured using Synergy™ H1 hybrid multi-mode microplate reader (BioTek Instruments, Inc.).
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bioRxiv - Microbiology 2022Quote: ... clear-bottomed 96-well plates using an excitation of 510 nm and emission of 600 nm using a Synergy H1 Hybrid Multi-Mode microplate reader with Gen5 software (Version 2.0, BioTek, Winooski, VT). Bacterial density was measured in tandem at OD600 ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric changes were read using an automated microplate reader with Gen5 Microplate Reader Software (Synergy H1 Hybrid Reader, BioTek, Vermont, USA).
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bioRxiv - Cancer Biology 2023Quote: ... The chromogenic reactions were stopped by adding 50 µL per well of 10% sulfuric acid (H2SO4) and absorbance values were measured by a Synergy H1 Hybrid microplate reader (BioTek, USA). Absolute IFNγ concentrations in test samples were interpolated from a standard curve and represented using GraphPad Prism v10 Software.
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bioRxiv - Bioengineering 2023Quote: ... Supernatants were analysed quantifying the at 405 nm in a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments,Winooski, VT, USA).
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bioRxiv - Cell Biology 2021Quote: ... The quantitative estimation of 2-NBDG fluorescence was carried out at 465/540 (ex/em.) and ATP production was measured at 560nm Luminescence on Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, USA) on Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was estimated on monitoring the optical density at 600 nm (Synergy H1 Hybrid Multi-Mode Reader, Biotek, Winooski VT, USA) over three biological replicates of cultures in plates (96 wells ...
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bioRxiv - Cancer Biology 2020Quote: ... was used for quantifying changes in mitochondrial membrane potential in live cells using a Synergy H1 Hybrid microplate reader (BioTek, Winooski, VT). The assay implements FCCP (carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Immunology 2022Quote: ... was measured at 450/620 nm using the Synergy H1 Hybrid Multi-Mode Reader with the Gen5 2.00 (version 2.00.18) software (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Microbiology 2019Quote: ... The maximum absorption wavelength of violacein was determined through full wavelength scanning from 230 nm to 750 nm by microplate reader (Synergy H1 Hybrid Multi-Mode Microplate Reader, BioTek, Winooski, VT).
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bioRxiv - Immunology 2019Quote: ... The supernatant was subjected to a BCA protein assay (Pierce Biotechnology, Waltham, MA) and measured on a Synergy H1 Hybrid Multi-Mode Reader (BioTek, Winooski, VT). After normalizing the samples to the lowest sample concentration through the addition of PBS ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was estimated on monitoring the optical density at 600 nm (Synergy H1 Hybrid Multi-Mode Reader, Biotek, Winooski VT, USA) over 4 independent replicates of cultures in plates (96 wells ...
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bioRxiv - Immunology 2022Quote: ... on a plate reader with excitation at 585 nm and emission at 530 nm (Synergy H4 Hybrid Microplate Reader, BioTek Instruments, Inc.). Whole-intestine permeability at each time point in each mouse was assessed by subtracting the average relative fluorescence units of baseline plasma and triplicate wells of PBS (sample blank ...
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bioRxiv - Microbiology 2022Quote: ... of the wild-type and mutant strains in 96 well microtiter plates (Surface Nunclon ™ Roskilde, Denmark) using a Sinergy H1 Hybrid Multi-Mode microplate reader (Biotek, USA). For this ...
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and continuously recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The immune complex was detected by adding tetramethyl-benzidine substrate solution and the absorbance was determined at 450 nm on a microplate reader (BioTek Synergy H1 Hybrid).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Varying concentrations of albumin was used to plot a reference standard curve at a scanning wavelength of 562 nm in a Synergy H1 Hybrid Multi-Mode Reader microplate reader (Biotek, Winooski, VT, USA). P450 enzyme activity was measured by the ability of the enzyme fractions to catalyze the O-deethylation of 7-ethoxycoumarin to 7-hydroxycoumarin according to methods of Adesanya et al [44 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TuCPR activity was measured as the reduction of cytochrome c by calculating absorbance at 550 nm over 5 minutes by with the kinetic mode in a Synergy H1 Hybrid Multi-Mode Reader microplate reader (Biotek, Winooski, VT, USA). The control reaction was the non-enzymatic reduction of cytochrome under the same conditions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ASChI hydrolysis was quantified by measuring changes in absorbance at 405 nm over 15 min using the Synergy H1 Hybrid Plate Reader with Gen5 2.0 software (BioTek Instruments, Winooski VT, USA). ChE activity was normalized against total protein concentration determined using the BCA assay according to the manufacturer’s directions (Pierce ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 70 μL/well were transferred to a black 96-well plate and fluorescence intensity was measured at 530/560 nm (excitation/emission) using a SynergyTM H1 hybrid multi-mode reader (BioTek, Bad Friedrichshall, Germany). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: ... Substrate cleavage was monitored continuously for 1 hour by detecting mTurquoise2 fluorescence (excitation: 434 nm / emission: 474 nm) using Synergy™ H1 hybrid multi-mode microplate reader (BioTek Instruments, Inc.). The first 15 min of the reaction was used to calculate initial velocity (V0 ...
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bioRxiv - Biochemistry 2020Quote: ... Elongation rates were measured using time-based scans on a plate reader (Biotek Synergy H4 Hybrid Multi-Mode Microplate Reader with Gen5 software, BioTek Instruments, Winooski, VT) at 25°C with excitation at 365 nm and emission detected at 407 nm.
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bioRxiv - Microbiology 2020Quote: ... were withdrawn at intervals 2.5 h during the bacterial growth for measurement of their optical density at 600 nm in 96-well plates (Synergy H1 Hybrid Multi-Mode Reader, Biotek, Winooski VT, USA). Cells in these aliquots were removed on centrifugation (10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Evolutionary Biology 2019Quote: ... by H2O2 was measured at an excitation wavelength of 545 nm and an emission wavelength of 590 nm using a Synergy H1 Hybrid plate reader (BioTek; Winooski, VT, USA), at 40°C in a 96-well plate using succinate ...
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bioRxiv - Microbiology 2021Quote: ... and ZsG expression was determined by fluorescence using a fluorescent plate reader (Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek, Winooski, VT, USA). Mean relative fluorescence units were normalized with vehicle control group (DMSO) ...
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bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
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bioRxiv - Genetics 2020Quote: ... 10 μl of ligand was added to each well and luminescence measurements were recorded immediately for 30 min at 1-min intervals (Synergy H1 Hybrid Multi-Mode Reader, BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2022Quote: ... The fluorescence of dichlorofluorescent dye was determined at excitation wavelengths of 480 nm and emission of 530 nm in a Synergy H1 Hybrid plate reader (Biotek; Winooski, VT, USA). To ensure fluorescence at various concentrations ...
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and growth was recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 μl EGFR-mTurq vesicles were transferred to 96-well plates and full fluorescence emission spectra were collected with a H4 Synergy Hybrid Microplate Reader (BioTek Instruments, Winooski, VT). The samples were excited at 430 nm with a 9 nm bandwidth and the emitted fluorescence was collected from 450 – 620 nm with a 9 nm bandwidth with 5 nm steps ...
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bioRxiv - Synthetic Biology 2023Quote: ... The concentration of GvpU was then measured using absorbance at 280 nm (OD280) on a Take3 plate (Agilent BioTek Synergy H4 Hybrid, Agilent) and adjusted to a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... plates were shaking for 30 min and the absorbance was measured at 570 nm in a multiwell plate reader Synergy H1 Hybrid Reader (Biotek Instruments Inc., USA).
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bioRxiv - Cell Biology 2023Quote: ... the stained cells were subjected to measurements of the absorbances of 455 nm and 525 nm at 37 °C using a plate reader equipped with kinetic mode (BioTek SynergyH4 Hybrid Reader).
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured at 10 seconds at Relative Luciferase Units (RLU) using a luminometer microplate reader (Biotek synergy H1 hybrid plate reader with Gen5v. Software, Biotek, Winooski, VT, USA).
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bioRxiv - Molecular Biology 2023Quote: ... and fluorescent readings (excitation = 485 nm, emission = 520 nm, gain = 70) were taken on a Synergy H1 Hybrid Multi-Mode Reader (BioTek, #11-120-535) using Gen5 Microplate Reader and Imager software (v2.04) ...
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bioRxiv - Bioengineering 2021Quote: ... an area scan of each well was taken at 600nm on a Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, Inc., Winooski, VT). Results were reported as normalized percent growth and percent growth reduction per mg polymer ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... Pyoverdine production was measured as the fluorescence at 400/460 nm excitation/emission on a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Winooski, VT, USA) of supernatants normalized against OD600 of ON cultures in King’s B medium ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescence polarization was monitored for two hours using the Cytation 5 multi-mode reader (BioTek) with the green filter set (485/20 nm for excitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunofluorescence images were acquired with the latter two and analyzed with the Gen5 (BioTek Instruments) or Zen Blue (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... medium (HuanKai Microbial, 024051) (28°C, shaken at 200 rpm under a normal aerobic atmosphere) and harvested at ∼0.8 OD600 (BioTek, Synergy H1 Hybrid Multi-Mode Reader). Bacterial cells were centrifuged at 5,000 ’g for 5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Fluorescence of the pPcdrA::mTFP1 reporter was measured in Wsp mutants in a fluorimeter (BioTek Synergy H1 Hybrid Reader, BioTek Instruments, Inc., Winooski, VT, USA) and in flow cells to confirm its activity resembled that of pPcdrA::gfpASV ...
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bioRxiv - Microbiology 2021Quote: ... Two hundred microliters of each sample were used to measure GFP fluorescence in a Synergy 2 Microplate Reader (Biotek) spectrophotometer (λexc=485 nm ...