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Citations for Biotek :
2801 - 2850 of 2988 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Fluorescence intensity (F) and the final OD600 of each sample was recorded on a Synergy 2 Multi-Mode Microplate Reader (BioTek) using excitation and emission filters with wavelengths of 485±20 nm and 535±20 nm ...
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bioRxiv - Neuroscience 2024Quote: ... The increase of absorbance was measured at 412 nm using Multimode microplate reader Synergy 2 (BioTek Inc., Winooski, VT, USA). For the calculation of the resulting measured activity (the percentage of inhibition I ...
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bioRxiv - Cell Biology 2024Quote: ... The mixture was allowed to equilibrate for 2 hr in the dark and the FP signal was measured by Synergy NEO HTS multi-mode microplate reader (Biotek). The background FP signal was measured by supplementing the reaction with 10 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... The fluorescence intensity of each well was measured at 545 nm using excitation at 276 nm immediately after mixing and followed for 2 hours using a SYNERGY microplate reader (Biotek).
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bioRxiv - Developmental Biology 2023Quote: ... The plate was incubated at room temperature for an additional 2 h and absorption was determined in a microplate reader (Synergy H1, BioTek) at λ = 570 nm/650 nm after automatic subtraction of background readings ...
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bioRxiv - Bioengineering 2023Quote: ... 100μL of media was removed from each well and deposited into an empty well before reading absorbance at 570nm and 600nm on a spectrophotometer (Epoch 2, BioTek). Each timepoint included biological triplicates for cell-seeded scaffolds (n=3) ...
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bioRxiv - Molecular Biology 2023Quote: Fluorescence polarization experiments were carried out at 25 °C in PBS supplemented by 2 mM TCEP and 0.05% Brij using a Synergy H4 (BioTek Instruments) plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescence changes at excitation/emission of 535/595 nm for JC-10 aggregates and at 485/535 nm for JC-10 monomers were monitored with a Synergy 2 Reader (BioTek). Mitochondrial membrane potential was quantified as the fluorescence of JC-10 aggregates/monomers (595/535 nm).
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bioRxiv - Bioengineering 2024Quote: ... The absorbance was measured at 450 nm for each sample and compared using a fluorescence microplate reader (BioTek, Synergy 2).
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bioRxiv - Bioengineering 2024Quote: ... The final co-cultures were then incubated at 37°C in an automated spectrophotometer (Biotek Synergy 2, Agilent Technologies, USA) for 48 hours ...
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bioRxiv - Microbiology 2024Quote: ... Culture growth was measured using chlorophyll a autofluorescence (excitation/emission: 440/680 nm) as a proxy for biomass using a Synergy 2 microplate reader (BioTek).
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bioRxiv - Microbiology 2024Quote: ... then diluted to A600=0.1-0.001 into either 5-10mL broth or into 96-well plates (Costar), and growth was monitored by measuring A600 every hour (broth culture) or every 15min (Epoch 2 or SynergyMx, (Biotek) plate readers. ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
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bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 4% PFA and stained with Hoechst nuclear stain before GFP-positive cells were counted using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) with the onboard software for calculations of percentage infected cells.
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then maintained at 37°C for 14-16 h post infection before automatic counting of GFP-positive cells using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) and using the onboard software for calculations of percentage infected cells.
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bioRxiv - Cancer Biology 2021Quote: ... at 1.5×104 cells/ well in 50 μl of DMEM supplemented with 10% FBS using BioTek™ MultiFlo™ FX Microplate Dispensers (BioTek Instruments). Cells were treated with an FDA approved library (Selleckchem ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TuCPR activity was measured as the reduction of cytochrome c by calculating absorbance at 550 nm over 5 minutes by with the kinetic mode in a Synergy H1 Hybrid Multi-Mode Reader microplate reader (Biotek, Winooski, VT, USA). The control reaction was the non-enzymatic reduction of cytochrome under the same conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescence intensity was measured at the vascular bundles of leaves 5 mm from the detachment sight using the Lionheart FX Gen5 image analysis mode (BioTek, Winnoski, VT, USA). Untreated plants were used as control with 6 biological repeats ...
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... isopropyl alcohol to quantitatively measure the density of the stain in different groups at 520 nm using microplate reader (Gene 5 Bio Tek Spectrometer, BioTek, northern Vermont, USA). Isopropanol was used as a blank (26)
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bioRxiv - Neuroscience 2020Quote: ... Immunofluorescence images were acquired using a Cytation 5 Cell Imaging Multi-Mode Reader and analyzed with Gen5™ software (BioTek, Winooski, VT, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Immunology 2019Quote: ... Recovered mAb-F(ab’)2 sample were evaluated for purity by SDS-PAGE and their concentration measured by Take5 (BioTek instruments).
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bioRxiv - Physiology 2020Quote: ... The samples were incubated 37 °C for 2 hours and absorbance was measured at 562 nm using a microplate spectrophotometer (BioTek Epoch).
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bioRxiv - Physiology 2021Quote: ... A 1.5 h kinetics curve was monitored at 37° using a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT, USA). The absolute amount of cleaved substrate was calculated by an AMC standard curve ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Immunology 2020Quote: ... The reaction was stopped with stop solution and absorbance was read at 450 nm using Epoch 2 microplate reader (BioTek, USA). Data are expressed as stimulation index (S.I ...
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bioRxiv - Neuroscience 2021Quote: ... Relative Light Units (RLU) were measured at 28°C using the Synergy 2 SL Microplate Reader with Gen5™ software (BioTek).
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence was measured for 2 h at excitation and emission on a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2023Quote: ... The cultures were grown under strong agitation at 37°C and analyzed in Synergy 2 Multi-mode microplate reader (BioTek Instruments). Relative Luminescence Units (RLU ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Microbiology 2024Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...