Labshake search
Citations for Biotek :
2451 - 2500 of 2988 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: The DNA concentration in the supernatant was measured by UV spectrometry at a wavelength of 260 nm (BioTek, Gen5 plate reader, USA). 96-well UV flat-bottom plates (Greiner UV Star 96 ...
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Cell Biology 2021Quote: ... 1 µl/ml HaloTag NanoBRET 618 ligand was added to the cell suspension and cells were seeded at a concentration of 8 x103 cells per well of a 384-well plate in quadruplicate (BioTek, Winooski, VT), with the remaining cells plated into a fresh 6-well tissue culture plate ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 µg total protein was used at 100 ng/µL and the RAS-GTP pull-down was measured using a Synergy H1 plate reader (BioTek, Winooski, US) in luminescent mode.
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bioRxiv - Bioengineering 2021Quote: ... after which the gels were transferred to a black 96-well plate and fluorescence readings were taken (BioTek Synergy H1 microplate reader).
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bioRxiv - Synthetic Biology 2021Quote: ... The plate was then incubated for 15 minutes at 37°C in an incubator with atmospheric CO2 and then transferred to a plate reader (BioTek; Synergy H1) with the lid removed ...
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bioRxiv - Microbiology 2021Quote: ... The absorbance of formazan at 490 nm is detected using an enzyme-linked immunosorbent assay (ELISA) plate reader (ELx808, BioTek Instruments, Inc.). Each measure was performed in triplicate.
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bioRxiv - Cancer Biology 2021Quote: ... FA values were measured with excitation wavelength 485 nm and emission wavelength 528 nm on a Synergy Neo multi-mode plate reader (BioTek, Winooski, VT). Data was fit to the following equation ...
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bioRxiv - Microbiology 2022Quote: ... Optical density was determined at 490 nm (OD490) and 630 nm (OD630) using an ELX800 plate reader (Biotek Instruments, Inc., Winooski, VT). Plates were incubated for 15 min (IgG ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase luminescence generated by 20 µl cell lysate per well in a 96-well plate format was recorded with a microplate reader (BioTek Synergy H1) using the Dual-Luciferase Reporter Assay System (Pierce) ...
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bioRxiv - Microbiology 2020Quote: ... was added to quench the reaction and the optical density at 490 nm of the culture plates was immediately measured using a Synergy LX multi-mode reader and Gen5™ microplate reader and imager software (BioTek®).
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... dried overnight and 100 µl/well of 100% methanol added to dissolve the crystal violet and the OD was read at 595 nm using a 96-well plate reader (Biotek Synergy H4). The 50% neutralizing titers were interpolated from optical density (OD ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to a 96-well plate and the OD695 was measured using a Cytation microplate reader (BioTek, VT, USA). Since clumping of cells was apparent in cultures of many strains ...
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bioRxiv - Physiology 2021Quote: ... Fluorescence intensity (λex = 495 nm; λem = 520 nm) was measured continuously in a Synergy Mx fluorescent plate reader (BioTek Instruments AG, Switzerland) at 37 °C before and after addition of various donors ...
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bioRxiv - Biochemistry 2021Quote: ... The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded onto a 96-well plate, and fluorescence was scanned (ex = 340 nm, em = 393 nm) by a microplate reader (Biotek Synergy Neo2). Four analytical replicates were collected.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescence of dextran was measured at 490 nm excitation/520 nm emission and fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4). Clearance volume in UT and FUS+MB treated Transwells was calculated as previously described [30] and data presented as fold change to UT.
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bioRxiv - Evolutionary Biology 2022Quote: ... Optical densities (OD600) were measured every 10 minutes during the incubation in a plate reader (Synergy HTX Multi-Mode Reader, BioTek Instruments, USA). The area under the growth curve (AUC ...
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bioRxiv - Biochemistry 2019Quote: ... A 200 µl of 95% ethanol solution was added to each micro-well and incubated for 30 minutes after which 100 µl from each well were transferred to a new 96-micro-well plate and recorded at absorbance of OD600 with a Synthergi HT (Biotek, Winooski, USA) device.
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bioRxiv - Cell Biology 2019Quote: ... according to manufacturer recommendations, and fluorescence (excitation 495 nm, emission 527 nm) determined using a Synergy H1 plate reader (BioTek Instruments, Inc.) in area scanning mode to account for uneven cell distribution ...
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bioRxiv - Genetics 2020Quote: ... Growth of yeast cells in liquid culture (in rich YPD media) was performed in 96-well format in a shaking and temperature-controlled plate reader (Biotek, Winooski, VT) and optical density (OD600 ...
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bioRxiv - Pathology 2020Quote: ... The reaction was stopped by 2N H2SO4 and the color was quantified at 450 nm using a Synergy Mx plate reader (BioTek; Winooski, VT).
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bioRxiv - Microbiology 2021Quote: ... The optical densities of cultures were measured at times 0 hours and 18 hours using a Synergy H1 plate reader (BioTek, Winooksi, VT). Growth was reported as the net growth of each strain amended with a nitrogen source after subtraction of the optical density in the 0 mM nitrogen condition ...
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bioRxiv - Microbiology 2021Quote: ... OD600 values were measured every 10 min for 7 h while plates were grown at 28°C with agitation (205 cpm) in a microplate reader (BioTek SYNERGY H1).
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions were subsequently distributed into 96-well plates and incubated at 37°C in a BioTek Synergy H1 microplate reader (BioTek, Winooski, VT). Subsequent OD600 measurements were taken every hour for 21 hours ...
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bioRxiv - Microbiology 2021Quote: ... Growth curves were followed over 48 h at 30°C by optical density readings every 15 min using a Synergy H1 Plate Reader (Biotek, Winooskim VT) with Gen5 Microplate Reader and Imager Software (Biotek ...
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bioRxiv - Microbiology 2021Quote: ... Optical density was determined at 490 nm (OD490) and 630 nm (OD630) using an ELX800 plate reader (Biotek Instruments, Inc., Winooski, VT). Plates were incubated for 15 min (IgG ...
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bioRxiv - Microbiology 2020Quote: ... pH 7 by reading absorbance at 486 nm every minute for 15 minutes at room temperature in microtiter plates (Biotek Synergy H1M). β-lactamase activity was calculated as initial reaction rate (Vmax ...
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Immunology 2022Quote: ... on a plate reader with excitation at 585 nm and emission at 530 nm (Synergy H4 Hybrid Microplate Reader, BioTek Instruments, Inc.). Whole-intestine permeability at each time point in each mouse was assessed by subtracting the average relative fluorescence units of baseline plasma and triplicate wells of PBS (sample blank ...
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bioRxiv - Microbiology 2022Quote: ... of the wild-type and mutant strains in 96 well microtiter plates (Surface Nunclon ™ Roskilde, Denmark) using a Sinergy H1 Hybrid Multi-Mode microplate reader (Biotek, USA). For this ...
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bioRxiv - Microbiology 2024Quote: ... The growth behavior of the different mutants of AB5075-VUB was assessed in liquid culture using the Cytation 1 plate reader (BioTek, United States). Each well of a 96-well plate was filled with 200 µL of bacterial culture diluted to an initial OD600 of 0.1 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Neuroscience 2024Quote: ... was measured in cell culture medium collected from the top and bottom chamber of the Transwell system (three technical replicates per Transwell) using a fluorescent plate reader (Biotek Synergy H4) and clearance volume calculated as previously described (37,38).
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bioRxiv - Microbiology 2023Quote: ... plates were briefly centrifuged at 500 xg and absorbance at 620 nm was then measured using a Cytation7 plate reader (Biotek, Winooski, VT). Raw absorbance values of the compounds were normalized to DMSO (0% inhibition ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and continuously recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... staining and washing steps (2x, wash volume 50 µl) were performed using automated liquid handling systems (405 plate washer, BioTek– Tempest, Formulatrix).
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated shaking at 37°C and the OD600 was recorded every 15 min for eight hours in a 96-well plate reader (Biotek Synergy Neo2). Cells producing HT-DivIB ...
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bioRxiv - Molecular Biology 2023Quote: ... 200nM substrates (unmodified or preubiquitylated) was mixed in and the extract was incubated at 30C on a plate reader (Biotek Synergy H1). Signal was detected every minute by a build-in monochromator (ex ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Microbiology 2023Quote: Analysis of the library of CRISPRi conditional mutants (derived from strain BCBMS14) was performed in a 96-well plate reader (Biotek Synergy Neo2). Overnight cultures were diluted 1:1000 in fresh media containing 10 µg/ml Cm ...
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bioRxiv - Biochemistry 2023Quote: ... Relative activities of the firefly and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... was added according to the manufacturer’s instructions and the total luminescence was measured for 20 min using Synergy Neo plate reader (BioTek, New Castle, DE). Then agonists (10 μM carbachol (carbamoylcholine ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μg/ml) and grown for 20 hours before measuring optical density (OD) using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT). We also conducted experiments with the E ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of GFP (excitation 485 nm/ emission 528 nm) and dTomato (excitation 555 nm/ emission 585 nm) fluorescence using a BioTek Synergy plate reader (BioTek, Winooski, VT). Transformants with at least 3-fold increase in GFP expression were selected as candidate Zn reporter strains.
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bioRxiv - Biochemistry 2023Quote: ... Reactions were started by addition of 1 mM Mg-ATP and NADH absorbance was monitored at 340 nm in a plate reader (Biotek PowerWave HT) at 26°C ...