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Citations for Biotek :
2251 - 2300 of 2549 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... were statically incubated at 30 °C for 48h and biofilm measurements were done as previously reported (41) using a Cytation5 plate reader (BioTek, Winooski, VT, USA). The experiment was replicated three times in total with 36 technical replicates each ...
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bioRxiv - Cell Biology 2023Quote: ... The NBD fluorescence spectra were recorded at 25°C with excitation at 470 nm and an emission range of 520 – 600 nm using a Cytation five imaging plate reader (Biotek Instruments, Winooski, VT). Lipid blanks were prepared at the highest and lowest pH ...
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bioRxiv - Biochemistry 2023Quote: ... Phosphorylation reaction progress was monitored by measuring fluorescence at excitation 530 nm and emission 590 nm every 2 minutes at 37 °C on a plate reader (BioTek Synergy Neo 2). The fluorescence units (RFU ...
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT). Library preparation and sequencing were done at the University of Michigan Advanced Genomics Core ...
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bioRxiv - Bioengineering 2023Quote: ... Absorbance was measured at 450 nm at the start of the incubation period and after 15 minutes using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA). ALP activity per explant was calculated using standards with known concentrations of p-nitrophenol ...
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bioRxiv - Cell Biology 2023Quote: ... the stained cells were subjected to measurements of the absorbances of 455 nm and 525 nm at 37 °C using a plate reader equipped with kinetic mode (BioTek SynergyH4 Hybrid Reader).
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured at 10 seconds at Relative Luciferase Units (RLU) using a luminometer microplate reader (Biotek synergy H1 hybrid plate reader with Gen5v. Software, Biotek, Winooski, VT, USA).
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bioRxiv - Microbiology 2024Quote: ... with ancestral or evolved phages were obtained from sequential OD600 readings using a microtiter plate reader (Epoch Microplate spectrophotometer; BioTek, Winooski, VT, USA). We added bacterial cultures at exponential growth to individual wells of a 96-well microtiter plate and mixed them with phage lysate at an MOI of 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Pyrene fluorescence was measured using time-based scans on a plate reader (Biotek Synergy H4 Hybrid Multi-Mode Microplate Reader with Gen5 software, BioTek Instruments, Winooski, VT) at 25°C with excitation at 365 nm and emission at 407 nm.
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bioRxiv - Immunology 2021Quote: ... Total RNA quantity and purity were assessed using a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States) with the Take3 microvolume plate accessory (absorbance read at 230 nm ...
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bioRxiv - Microbiology 2021Quote: ... 75,000 effector cells/well were added to the plate and incubated for 6 h at 37°C.The luciferase substrate was added in the dark and the luminescence activity measured after 5 minutes using a Synergy Hybrid Reader (BioTek). The data was analysed using GraphPad prism 7.
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bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
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bioRxiv - Immunology 2020Quote: ... Images were acquired in random fields at 20x magnification in brightfield and GFP (469nm/525nm) channels using Cytation 5 Imaging Multi-Mode Reader (Biotek) at 3 minutes intervals for 6 hours ...
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bioRxiv - Plant Biology 2019Quote: ... The image acquisition and analysis of roots and shots were performed in Cytation™ 5 cell imaging multimode reader and Gen3.03 software (BioTek). Venus was excited at 488 nm and detected at 498-530 nm ...
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bioRxiv - Cancer Biology 2021Quote: The proliferation assay was performed by DAPI staining of the cell nuclei and automatically counted by a CytationTM 5 Cell Imaging Reader (BioTek). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 12000 rpm for 5 minutes and the absorbance of supernatants was read at 475nm in the Synergy-H1 microplate reader (BioTek). Absorbance values were compared to the standard curve of synthetic melanin (155343 ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensity of each well containing AβpH was obtained on a Cytation™ 5 imaging multi-mode reader (BioTek Instruments) at 443/505 nm excitation/emission wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... The concentration of Aβ1-42 was determined by the absorbance value detected using a microplate reader (BioTek Cytation 5, US) at 450 nm and 590 nm.
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bioRxiv - Cell Biology 2021Quote: ... Bright field images of colonoids were collected at three different focal planes over the Matrigel drop using a Cytation 5 imager (BioTek). Colonoids that were in focus were scored as either spheroid or non-spheroid ...
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bioRxiv - Microbiology 2021Quote: ... The fluorescence signals were read at a 365 nm excitation wavelength and a 450 nm emission wavelength using a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) and a standard curve was created ...
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bioRxiv - Microbiology 2020Quote: ... erythromycin was added to the culture to a final concentration of 15 µg/ml (5-fold MIC) and growth curves were acquired for 20 h using a microplate spectrophotometer (EON, Biotek) by measuring the OD578 every hour after 30 sec of linear shaking.
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bioRxiv - Cell Biology 2020Quote: Cells were imaged in either a 96- or 24-well dish every two or four hours for up to five days using the Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) driven by Gen5 software (Biotek) ...
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bioRxiv - Microbiology 2022Quote: ... The plates were further incubated for six hours at 37°C in a 5% CO2 atmosphere and then analyzed spectrophotometrically (Synergy H1, Biotek) at 640 nm ...
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bioRxiv - Physiology 2022Quote: ... Images of each well were captured every 24 hours for the duration of the experiment using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at room temperature and the imaging acquisition was performed by using DAPI 355nm and RFP 558nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The percentage of positive cells was calculated with a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... The methanol was removed and the CSK buffer was added before imaging nucleus areaacquisition by using DAPI 355nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The Nucleus area of cells was acquired by a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... Cell imaging acquisition was performed by using GFP 488nm and DAPI 355nm on Cytation 5 Cell Imaging MultiMode Reader (BioTek).
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bioRxiv - Biophysics 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Microbiology 2020Quote: ... 5 minutes) to remove the beads and the absorbance at 570 nm was measured from the resulting supernatant (Biotek Eon). One million amine plus phosphonate beads were analyzed ...
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bioRxiv - Microbiology 2022Quote: ... 75 µL of culture media was added for each well and the expression of EGFP was monitored every 20 min for 28 hours at 37 °C in Cytation 5 (BioTek) provided with 5% CO2 ...
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bioRxiv - Systems Biology 2022Quote: ... the wells were imaged using the TagBFP (Excitation-390nm, Emission-447nm) and RFP filters (Excitation-531/40 nm, Emission-593/40 nm) in Cytation 5 (Biotek). Each image was flatfield corrected and background subtracted using CellProfiler ...
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bioRxiv - Immunology 2022Quote: ... and the cell plate was sat in dark for 1 h before absorbance at 517 nm was recorded using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). All values were normalized to the background ...
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bioRxiv - Biochemistry 2019Quote: ... Developing reagent and quench reagent were delivered sequentially by an EL406 automated multi-channel liquid dispenser with 5 µL Dispense Cassette (BioTek). Absorbance was determined at λ= 620 nm (A620 ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was incubated in the dark at room temperature for 5 minutes followed by fluorescent quantification using an excitation/emission of 485/520 nm (BioTek Cytation5 spectrofluorometric plate reader) ...
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bioRxiv - Microbiology 2019Quote: ... The OD600 nm of the cultures was measured after 24 h incubation at 37°C in a 5% CO2 aerobic atmosphere with a Synergy 2 multimode microplate reader (BioTek). The experiment was performed at least four times.
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bioRxiv - Molecular Biology 2019Quote: ... LDL uptake was determined by quantification of the fluorescence signal using a multi-mode fluorescent microplate reader (Cytation 5, Biotek), and corrected for the protein contents in the same lysates as determined with the BCA assay ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was incubated at room temperature for 5 min and the absorbance (A650) was read using a Synergy H4 microplate reader (BioTek). Inhibition was observed with varying concentrations of 1.
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bioRxiv - Microbiology 2019Quote: ... or RFP (excitation 555 nm, emission 610 nm) relative fluorescence units (RFU) recorded every 5 min with the Gen5 (BioTek) software ...
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bioRxiv - Neuroscience 2020Quote: ... The intrinsic tryptophan fluorescence of L-PGDS was measured using Cytation 5 cell imaging multi-mode (Biotek Instrument Inc., USA) reader with λex=295 nm and λem=340 nm ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence levels were recorded every 5 minutes over a period of 500 minutes using a Synergy H1 hybrid reader system (Biotek) at excitation/emission wavelengths of 488/515 nm ...
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bioRxiv - Neuroscience 2021Quote: ... Changes in medium pH during hypercapnia were measured by Phenol Red absorbance at 415 and 560 nm using the Cytation 5 Multi-Mode Reader (BioTek)89 ...
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bioRxiv - Immunology 2020Quote: ... Cells were imaged on a LSM 780 confocal microscope (Zeiss) with a 40 or 63x oil-immersed objective with Airyscan or a Cytation 5 (BioTek) with a 20x objective ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Biochemistry 2021Quote: ... OD600 as well as GFP fluorescence (485 nm excitation, 528 nm emission) was measured in a microplate reader (Cytation 5 BioTek) and the fluorescence signal was normalized to OD600 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with Hoechst 33342 and counted for GFP+ and total cells with Cytation 5 high-content live-cell imaging system (Biotek) using a 4x objective.
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bioRxiv - Bioengineering 2020Quote: ... Well plates were always protected from light and fluorescence measurements were carried out at 37°C and 5% CO2 at Cytation 3 (Cell Imaging Multi-Mode Reader) from Biotek® (USA) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Baseline fluorescence intensity was measured with excitation at 350 nm and emission at 435 nm and 500 nm for 5 min at 37°C on a Synergy H1 spectrophotometer (BioTek). Cells were then treated with 10% ethanol as a membrane fluidizer ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of the cell suspension and the BacTiter-Glo reagent were mixed and incubated for 5 min before measuring luminescence using a Synergy Mx Monochromator-Based Multi-Mode Microplate Reader (BioTek). Signal intensities were corrected for cell numbers by dividing the luminescence signal by the OD 595 nm of the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were diluted back to a calculated OD 600 of 0.05 (replicated in 2-5 wells for each culture) and their growth measured using an Epoch microplate spectrophotometer (BioTek, VT) at 37 °C with continuous shaking ...