Labshake search
Citations for Biotek :
2201 - 2250 of 2549 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Isolated gDNA was checked for quality and concentration by gel electrophoresis and by spectrophotometry using a Take3 Micro-Volume Plate (BioTek, Winooski, VT, USA). Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB) ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated ∼5 min at room temperature, and read by fluorescence (λex = 480 nm, λem = 520 nm) using a Synergy H1 plate reader (BioTek, Winooski, VT, USA).
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bioRxiv - Biochemistry 2019Quote: ... Plates were agitated at 25°C for 10 min and absorbance was recorded at 590 nm using a multi-well plate reader (Biotek-Epoch; Biotech Instruments). The average absorbance value of five replicate wells was used for each set and each experiment was repeated thrice ...
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Microbiology 2021Quote: ... cells from overnight cultures were diluted to a fixed starting OD600nm of 0.01 in fresh LB medium supplemented with MgSO4 in 96-well cell culture plates (CellStar) and grown in a microplate reader Synergy2 (BioTek Instruments, Vt, USA) at maximum rpm at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Culture densities were monitored over time by measuring light scatter at 600 nm using either a Synergy HTX multi-mode plate reader (Biotek, Winooski, VT, USA) or an Ultraspec 10 density meter (Biochom ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ASChI hydrolysis was quantified by measuring changes in absorbance at 405 nm over 15 min using the Synergy H1 Hybrid Plate Reader with Gen5 2.0 software (BioTek Instruments, Winooski VT, USA). ChE activity was normalized against total protein concentration determined using the BCA assay according to the manufacturer’s directions (Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability and cell proliferation were assessed by measuring the optical density at 570 nm in a plate reader (BioTek Eon,11-120-611).
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bioRxiv - Biochemistry 2020Quote: ... Elongation rates were measured using time-based scans on a plate reader (Biotek Synergy H4 Hybrid Multi-Mode Microplate Reader with Gen5 software, BioTek Instruments, Winooski, VT) at 25°C with excitation at 365 nm and emission detected at 407 nm.
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bioRxiv - Microbiology 2020Quote: ... were withdrawn at intervals 2.5 h during the bacterial growth for measurement of their optical density at 600 nm in 96-well plates (Synergy H1 Hybrid Multi-Mode Reader, Biotek, Winooski VT, USA). Cells in these aliquots were removed on centrifugation (10 min ...
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bioRxiv - Microbiology 2020Quote: At least three replicates per strain and growth condition were grown in 200 μl of LB alone or supplemented with canavanine in a 96-well plate at 30°C with 140 rpm shaking in a BioTek Eon Microplate Spectrophotometer (BioTek, Winooski, VT, USA). The A600 was measured at 10 minutes intervals ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and the luminescent signal was recorded using a BioTek® Cytation3™ UV–vis multi-well plate imaging reader (BioTek, Winooski, Vermont, USA).
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bioRxiv - Cell Biology 2022Quote: ... only 10 μL of MTS reagent was added to plate wells because there was not enough reagent to add 20 μL to all 96 wells.) End-point absorbance measurements were taken with a plate reader at 490 nm (BioTek Instruments, Agilent Technologies).
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bioRxiv - Cell Biology 2022Quote: ... 1 μl from overnight culture was transferred to the YPD and growth was monitored in 96-well plate using Epoch2 (BioTek, Winooski, VT, USA) kinetic growth analyzer by analyzing optical density of OD600.
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bioRxiv - Physiology 2021Quote: ... Lysates were incubated for 15 min at 37°C in presence of 10 μM SSP4 and fluorescence intensity (λex = 495 nm; λem = 520 nm) was measured in a Synergy Mx fluorescent plate reader (BioTek Instruments AG, Switzerland)
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bioRxiv - Molecular Biology 2021Quote: ... YP + α-KG (10g/l) or YPD (0.02% glucose) + α-KG and growth was monitored in 96 well plate using Epoch2 (BioTek, Winooski, VT, USA) kinetic growth analyzer by analyzing optical density of OD600 ...
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bioRxiv - Systems Biology 2019Quote: ... Assay plates were incubated for 24 h (30°C, 160 rpm) in a Cytation3 cell imaging multimode reader (BioTek Instruments, Winooski, VT, USA). At 24 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... by H2O2 was measured at an excitation wavelength of 545 nm and an emission wavelength of 590 nm using a Synergy H1 Hybrid plate reader (BioTek; Winooski, VT, USA), at 40°C in a 96-well plate using succinate ...
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bioRxiv - Microbiology 2019Quote: ... The plate was sealed with optically-clear tape and incubated at 30°C in the dark without shaking in a Synergy HT plate reader (Biotek, Winooski, VT, USA). Optical density at 405 nm (OD405 ...
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bioRxiv - Microbiology 2021Quote: ... These aerobic cultures were monitored periodically every 60 min for 37 °C growth at 600 nm in Synergy H1 plate reader (BioTek Instruments Inc, VT).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were allowed to equilibrate for 10 min post-lysis followed by luminescence reading using a Cytation3 multimode plate reader (BioTek, Winooski, VT, USA) set to luminescence ...
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bioRxiv - Microbiology 2021Quote: ... and ZsG expression was determined by fluorescence using a fluorescent plate reader (Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek, Winooski, VT, USA). Mean relative fluorescence units were normalized with vehicle control group (DMSO) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The OD600 was measured at 10 min intervals for 20 hr at 32°C in orbital shaking mode on a SynergyH1 plate reader (Biotek, Winooski, VT, USA). Growth data for each strain was obtained from three replicate wells and three independent cultures ...
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bioRxiv - Evolutionary Biology 2021Quote: ... treatment populations were exposed to UV during the exponential phase by monitoring their growth/OD600 in a plate reader (Synergy HT, BIOTEK Winooski, VT, USA) at 20-minute intervals ...
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bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
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bioRxiv - Bioengineering 2022Quote: ... Absorbency was measured immediately after adding stop solution at 450 nm on a plate reader (Citation 1 imaging reader, BioTek, Winooski, VT, US).
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bioRxiv - Immunology 2022Quote: ... The fluorescence of dichlorofluorescent dye was determined at excitation wavelengths of 480 nm and emission of 530 nm in a Synergy H1 Hybrid plate reader (Biotek; Winooski, VT, USA). To ensure fluorescence at various concentrations ...
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bioRxiv - Microbiology 2022Quote: ... The growth was monitored by measuring optical density at 600 nm (OD600nm) using a Synergy plate reader (Biotek Instruments, Inc, Winooski, VT, USA). A maximum specific growth rate (h-1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
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bioRxiv - Physiology 2023Quote: ... Growth was assayed by measuring OD600 at intervals of 30 minutes for 48–72 hours on plate readers (Biotek Instruments Inc., VT, USA). Cultures were incubated at 37°C and at 237 cpm ...
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bioRxiv - Microbiology 2022Quote: ... Optical density at 600 nm was measured over the next 18-24 hours using a plate reader (BioTek Synergy neo2 multi-mode reader) at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and Abs750nm of all 42 plates was measured after 65h exposition (4-6 hours after light on) with an absorbance microplate reader Epoch (BioTek Instruments, VT, USA). 9 data points per well were analyzed and averaged using software Gen5 2.0 (Biotek ...
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then diluted 1:100 and incubated with increasing concentrations of antibiotics in the Synergy HT BioTek plate reader (BioTek Instruments Inc., USA) at 37°C for 16 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Absorbance was measured at 450 nm at the start of the incubation period and after 15 minutes using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA). ALP activity per explant was calculated using standards with known concentrations of p-nitrophenol ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 10 minutes and then fluorescent measurement was applied (Excitation at 550 nm, emission 650 nm) at Biotek Synergy H1 plate reader (Biotek, Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and growth was recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 μl EGFR-mTurq vesicles were transferred to 96-well plates and full fluorescence emission spectra were collected with a H4 Synergy Hybrid Microplate Reader (BioTek Instruments, Winooski, VT). The samples were excited at 430 nm with a 9 nm bandwidth and the emitted fluorescence was collected from 450 – 620 nm with a 9 nm bandwidth with 5 nm steps ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT). Library preparation and sequencing were done at the University of Michigan Advanced Genomics Core ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT). RNA was reverse transcribed into cDNA using the cDNA Superscript Mix (Quanta Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... The concentration of GvpU was then measured using absorbance at 280 nm (OD280) on a Take3 plate (Agilent BioTek Synergy H4 Hybrid, Agilent) and adjusted to a final concentration of 1 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Absorbance was measured directly after mixture and then every 10 minutes to ensure values were within standard curve range using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at 492 nm ...
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bioRxiv - Immunology 2023Quote: ... the plate was washed 3x with Wash Buffer (1x PBS and 0.05% (v/v) Tween-20) using a magnetic plate washer (Biotek 405TS, Biotek Instruments GmbH). To detect bound IgG RSV antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... plates were shaking for 30 min and the absorbance was measured at 570 nm in a multiwell plate reader Synergy H1 Hybrid Reader (Biotek Instruments Inc., USA).
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bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
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bioRxiv - Microbiology 2023Quote: ... were statically incubated at 30 °C for 48h and biofilm measurements were done as previously reported (41) using a Cytation5 plate reader (BioTek, Winooski, VT, USA). The experiment was replicated three times in total with 36 technical replicates each ...