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Citations for Biotek :
101 - 150 of 265 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The supernatant of each plate was then carefully discarded and cell viability determined by crystal violet assay reading the absorbance at 595 nm (Synergy HTX Biotek) as described41.
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bioRxiv - Microbiology 2020Quote: ... tissue culture supernatants were collected at 48 h post-infection and Nluc expression was measured using a luciferase assay and a Synergy LX microplate reader (BioTek). Individual wells from three independent experiments conducted in quadruplicates were used to calculate the average and standard deviation (SD ...
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bioRxiv - Microbiology 2020Quote: ... tissue culture supernatants were collected at 48 h post-infection and Nluc expression was measured using a luciferase assay and a Synergy LX microplate reader (BioTek). Individual wells from three independent experiments conducted in quadruplicates were used to calculate the average and standard deviation (SD ...
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bioRxiv - Cancer Biology 2020Quote: ... The number of cells was determined at the end of the run using the SRB assay as previously described (62) or in plate cell counting by Cytation 1 imaging reader (Biotek).
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bioRxiv - Cell Biology 2022Quote: ... The plate was then cultured for 1 day before washing with assay buffer (1x HBSS, 10 mM HEPES, pH 7.4) in a ELx405 CW plate washer (BioTek Instruments). The cells were then incubated with 1.25 µM calcium indicator Fluo-8 AM (AAT Bioquest ...
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bioRxiv - Microbiology 2022Quote: ... Batch growth kinetic assays were performed in 96-well plates and growth was measured using a micro-well plate reader (Biotek). For this ...
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bioRxiv - Pathology 2023Quote: ... The assay was performed at 37°C in 96-well format and the fluorescence recorded using Synergy H1 plate reader (BioTek). Mir05 buffer with 1 mM malate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cells were washed with Assay Buffer before being photographed and determining a double fluorometric measurement using a microplate fluorescence reader (Synergy HT BioTek). The autophagosomes were quantified by normalizing green fluorescence (Cyto-ID ...
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bioRxiv - Biophysics 2023Quote: ... or mVenusH148Q/I152L and mTq2 (for I-/Cl- antiport assay) were measured in a time dependent manner using Synergy Neo2 (Agilent/BioTek) and the data analyzed offline as described previously (Wasano et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Assay plates were measured for the formazan product at 490 nM using a Synergy 2 plate reader (BioTek, Winooski, VT). Net lactate production was determined by subtracting lactate in the initial media from cancer cell’s lactate production at each time point during the experiments.
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bioRxiv - Plant Biology 2023Quote: ... Supernatant was collected and fluorogenic assay was performed in a plate reader (Synergy HT Plate Reader (BioTek, Winooski, VT, USA). GUS assay buffer was pre-warmed at 37°C and 25 µl of plant extract (supernatant from sample ...
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bioRxiv - Immunology 2024Quote: ... and was read at OD405 and recorded in a kinetic assay every minute for 1 hour using a Synergy MX microplate reader (BioTek).
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bioRxiv - Cell Biology 2024Quote: ... The assay was quantified by spectrophotometry at the absorbance of 540 nm (Agilent BioTek Epoch, Santa Clara, CA, United States).
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bioRxiv - Systems Biology 2021Quote: Vmax assays for comparison of yeast and human cell extracts were carried out with freshly prepared extracts via NAD(P)H-linked assays at 37 °C in a Synergy H4 plate reader (BioTek™). The reported Vmax values represent total capacity of all isoenzymes in the cell at saturating concentrations of all substrates and expressed per extracted cell protein ...
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bioRxiv - Bioengineering 2022Quote: ... Mineral precipitation in the mineralization solution was determined by measuring the optical density of 100 μl sample in a 96-wells assay plate at 600 nm using a plate reader (Synergy™ HTX, Biotek).
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bioRxiv - Systems Biology 2022Quote: ... All assays were performed in triplicate wells at 25°C in a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT) with excitation and emission wavelengths of 360 and 460 nm ...
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bioRxiv - Bioengineering 2021Quote: ... The viability and cytotoxicity of the groups were evaluated using Calcein/PI assay and measured with a microplate reader (H1M, BioTek, USA).
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bioRxiv - Bioengineering 2021Quote: ... The proliferation activities of the cells in the groups were evaluated using the MTT assay and measured with a microplate reader (H1M, BioTek, USA).
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bioRxiv - Biochemistry 2024Quote: ... The assay was performed at 24°C using a Synergy HTX multi-mode reader (ex/em = 360 nm/460 nm; BioTek Instruments). Fractions containing proteasome activity were pooled and loaded onto a 5 mL anion exchange HiTrap DEAE FF column (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The number of worms in each well was confirmed at the end of the assay with a Cytation-1 Imaging Multi-Mode Reader (BioTek Instruments). Data were analyzed using Agilent/Seahorse software Wave Desktop and Controller (v2.6.1 ...
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bioRxiv - Cancer Biology 2024Quote: ... This was also a kinetic assay from 0-14hrs with fluorescent readouts (excitation/emission = 490/525nm; Biotek Synergy H4 hybrid reader).
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bioRxiv - Evolutionary Biology 2020Quote: ... HP Plant DNA Mini Kit (Omega Biotek) following the manufacturer protocol and libraries were prepared with the same enzymes and protocol as for insects ...
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bioRxiv - Developmental Biology 2022Quote: ... purified (EZNA Gel Extraction Kit, Omega Biotek), quantified (NanoDrop Technologies Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... ® SP Plant DNA Kit (Omega Biotek). Partial genomic sequences of CpPT1 were PCR amplified in these preparations using SapphireAmp Fast PCR Master Mix (Takara ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA Kit (Omega Biotek, Norcross, GA). RNA (500 ng ...
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bioRxiv - Plant Biology 2023Quote: ... Cycle Pure Kit (Omega Biotek, Georgia, USA) and were re-quantified with the Qubit HS assay ...
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bioRxiv - Genomics 2023Quote: ... Total RNA Kit I (R6834 Omega BioTek).
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bioRxiv - Neuroscience 2020Quote: ... and the fluorescence intensity was measured at the end of the assay using a microplate reader (BioTek, Ex/Em = 350/440 nm).
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bioRxiv - Microbiology 2021Quote: ... The absorbance of formazan at 490 nm is detected using an enzyme-linked immunosorbent assay (ELISA) plate reader (ELx808, BioTek Instruments, Inc.). Each measure was performed in triplicate.
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bioRxiv - Biochemistry 2021Quote: ... The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... four days after transfection and luciferase activity was measured using the Nano-Glo® Dual-Luciferase® Reporter Assay System using the Synergy Neo2 multi-mode reader (BioTek). Nanoluciferase activity was normalized to that of the firefly luciferase.
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bioRxiv - Immunology 2022Quote: ... and luminescence in relative light units (RLUs) was measured using a Synergy Neo2 Multi Mode Assay Microplate Reader (Biotek Instruments, Winooski, VT). Three biological replicates were performed for each antibody or MB ...
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bioRxiv - Biochemistry 2023Quote: ... The color development in each assay well was monitored at a series of time points using an automated microplate reader (BioTek, Synergy-HTX). A420nm values in the linear course of color development were used to plot titration curves and the antibody dilution required for 50% maximum binding was quantify for the binding affinity of mAb TnI-1 to its specific epitope in the TnT constructs ...
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bioRxiv - Immunology 2024Quote: ... Peptide loading was determined by fluorescence quantification using a modified fluorescamine peptide quantification assay in the presence of ACN (Ex/Em: 390/465 nm, Biotek Cytation 5)104.
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bioRxiv - Plant Biology 2022Quote: ... SP Plant DNA Kit (Omega BioTek, Norcross, GA) (Pyne et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA Kit 1 (Omega BioTek, Norcross, GA). In conditions where we wanted to observe the effects in myotubes ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed in 3.7% formaldehyde and the assay was read on Lionheart FX automated fluorescent microscope (BioTek Instruments, Inc., Winooski, VT, USA). The total number of cells per well was counted using the Object Count feature in Nikon Elements AR Analysis version 5.02 by a blinded observer ...
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bioRxiv - Microbiology 2022Quote: ... The growth curve assays were performed in non-treated non-tissue culture plates using a plate reader (BioTek Synergy neo2 multi mode reader) in 200μl of 7H9 medium at 37°C starting at an Optical Density at 600nm (OD600 ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of the medium of the upper compartment was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µl of the medium was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we performed automated growth-assays on each of the 24 revived populations using a well-plate reader in multiple environments (Synergy HT, BIOTEK ® Winooski, VT, USA). Using optical density at 600 nm as the measure of population density ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... or the E.Z.N.A Total RNA kit I (Omega-Biotek), according to the manufacturer’s protocols.