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Citations for Biotek :
1 - 50 of 265 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... lungs were lavaged with 1 ml of sterile PBS to evaluate lung vasculature permeability via microplate reader (495nM ex/519nm em) (Syngery Mx, BioTek Instruments, Inc., Winooski, VT) and tissues taken for histological analysis ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were incubated at room temperature for 90 min and subsequently the parallel (Fpara) and perpendicular (Pperp) fluorescence was measured in a Synergy H1 fluorescene reader (BioTek). The following formula was used to calculate the fluorescence polarization (FP ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... Polarization (expressed as milli-Polarization (mP) units) is derived from measured parallel and perpendicular emission intensities (I) and was calculated by the Gen5 software (BioTek/Agilent); Polarization can also be calculated manually using Equation 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
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bioRxiv - Cancer Biology 2021Quote: ... The Assay and Universal primers supplied with Norgen’s HR kit (Norgen’s Biotek Corp., Canada) were used during both PCR and RT-PCR reaction set up ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Neuroscience 2020Quote: ... The rats’ insulin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Hangzhou Eastbiopharm Co., Ltd., Hangzhou, China) and a spectrophotometer (Biotek Epoch™ Microplate Spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and gaussia luciferase activity was determined using Gaussia Luciferase Glow Assay Kit (Thermo) on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA concentration measurements were performed using the Qubit dsDNA high sensitivity assay kit on the Synergy H1 Hybrid Microplate Reader (BioTek). Libraries were generated using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid hydroperoxides were extracted from neuronal lysates and neuron-conditioned media and analyzed using a lipid hydroperoxide assay kit according to the manufacturers protocol and Synergy Mx Multi-Mode Microplate Reader (BioTek). VAMP7 and syntaxin 4 knockdown neurons used for the lipid hydroperoxide assay were transduced at an MOI 7.5.
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Immunology 2024Quote: ... 24 h post-dsRNA transfection cells were treated with Pierce™ Firefly Luc One-Step Glow Assay Kit and luminescence of firefly luciferase was measured in Synergy H1 (BioTek) microplate reader.
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bioRxiv - Microbiology 2021Quote: ... The quantity of DNA in each sample was fluorescently measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) using a Synergy H1 Microplate reader (BioTek, Cat # BTH1M).
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bioRxiv - Microbiology 2022Quote: ... The quantity of DNA in each sample was measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) read with a Synergy H1 Microplate reader (BioTek, Cat # BTH1M). Sequence libraries were prepared from the extracted DNA samples using the Nextera DNA Flex Library Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant was measured using an iron assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.).
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant using a lipid hydroperoxide assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.). For the iron depletion assay ...
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... and three N128-138 peptides of the SARS-CoV-2 N-protein with the GFP-tagged G3BP1NTF-2 protein was determined using a fluorescence-based PPIs assay.92–94 The assay was performed using a Synergy HTX multimode plate reader (Agilent BioTek) equipped with Gen5 software ...
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bioRxiv - Cell Biology 2022Quote: ... the cells for both assays were washed five times with the respective assay buffer using an automated BioTek 405 TS microplate washer (BioTek Instruments, Winooski, VT). For the final aspiration after the last wash ...
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bioRxiv - Plant Biology 2022Quote: ... Assays used a Synergy H1 Microplate Reader (BioTek Instruments, Inc.) using 1 s integration time ...
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bioRxiv - Biochemistry 2024Quote: ... Assays were performed on a Synergy Neo2 plate reader (BioTek). Binding curves were fit using a One Site ...
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bioRxiv - Cell Biology 2022Quote: ... both assays were measured with a plate reader (BioTek, Synergy 2) at a 570 nm wavelength ...
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bioRxiv - Molecular Biology 2021Quote: This assay was performed with two different equipment: Synergy H1 (BioTek) with 96-well plate and LumiCycle luminometer (Actimetrics ...
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bioRxiv - Microbiology 2022Quote: ... Assay plates were loaded into a Biotek Cytation5 (Biotek, Vermont, USA) pre-equilibrated to 37 °C and 4% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... The plate was sealed with a Breathe-Easy sealing membrane (Diversified Biotek #BEM-1), and baseline luminescence was measured with a Synergy HTX Multi-Mode Microplate Reader (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The colorimetric assay was analyzed by a Synergy HT plate reader (BioTek).
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bioRxiv - Microbiology 2021Quote: ... The luminescence assay was measured using Biotek Synergy H1 microplate reader (Biotek) and expressed as relative luminescence units (RLU) ...
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bioRxiv - Neuroscience 2024Quote: ... a fluorometric assay was conducted using a Synergy TM2 fluorimeter (Biotek®). Fluorescence emission was recorded with an excitation wavelength of 360 nm and emission at 450 nm ...
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bioRxiv - Microbiology 2022Quote: ... Assays were imaged using a Cytation 3 high-content plate reader (BioTek). Intracellular parasites ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The antimicrobial assays were done in a microplate reader (Synergy H1 BioTek) at 30 °C with a 600 nm wavelength for 8 hours with a double orbital shaking step after each OD reading cycle (every 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were allowed to dry before being imaged using the Lionheart FX Automated Microscope (Biotek). Migrated cells were then counted using ImageJ (v1.52k ...
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bioRxiv - Biochemistry 2023Quote: ... Plate-based assays were conducted on the Synergy H1 Microplate Reader from BioTek Instruments (USA) ...
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bioRxiv - Plant Biology 2023Quote: ... Assays were performed on a Synergy HT plate reader (Biotek Instruments Inc, USA) at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... Absorbance of the colorimetric assay was quantified by Epoch Microplate Spectrophotometer (Biotek Instruments).
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bioRxiv - Cell Biology 2022Quote: ... Assay detection was performed using the Synergy Mx Multi-Mode Microplate Reader (BioTek).
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bioRxiv - Biochemistry 2024Quote: Peroxidase activity assays were carried out using a Synergy 2 microplate reader (BioTek) at room temperature by following the rate of oxidation of 2,2′-azino bis (3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Cell Biology 2020Quote: ... All luciferase assays were measured using a Synergy HT Microplate Reader (BioTek, Winooski, VT) and performed in triplicate.
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence was measured using a Synergy Neo2 Multi-Mode Assay Microplate Reader (Biotek Instruments) while employing a Texas Red filter ...
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bioRxiv - Cell Biology 2022Quote: ... to measure luminescence using a Synergy Neo2 Multi-Mode Assay Microplate Reader (Biotek Instruments). Data was plotted and half-cytotoxic concentration (CC50 ...
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bioRxiv - Biochemistry 2022Quote: ... Plate-based fluorescence assays were conducted on the Synergy H1 Microplate Reader from BioTek Instruments (USA) ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence assays were conducted on a Synergy™ 4 microplate reader (BioTek, Winooski, VT) using 3 µl of the in vitro translation reaction mixture in 150 µl of luciferase assay reagent (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Resulting absorbance of both assays were determined on a Synergy HT plate reader (BioTEK, Beun de Ronde ...
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bioRxiv - Neuroscience 2020Quote: ... Assays were read using a BioTek Synergy 2 Plate Reader (BioTek Instruments, Inc., Winooski, VT).
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bioRxiv - Cell Biology 2022Quote: ... and luminescence was read using a Synergy Neo2 Multi-Mode Assay Microplate Reader (Biotek Instruments). Data was plotted and half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Biophysics 2020Quote: ... The assay has been adapted to a 96-well format utilizing Synergy 4 (BioTek Instruments) or SpectraMax M3 (Molecular Devices ...
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bioRxiv - Plant Biology 2022Quote: ... All assays were carried out with a Synergy HT plate reader (Biotek Instruments Inc., USA) at 25° C ...
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bioRxiv - Immunology 2023Quote: ... The assay was performed in 96 – half area white plates in Synergy H2 (Biotek, USA) microplate reader.