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Citations for Biotek :
1201 - 1250 of 1433 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... blue and red fluorescence images were acquired per well at 20X every hour for 12h using the Cytation 5 cell imaging reader (Biotek). Imaging analysis was performed with Gen5 software (Biotek) ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty phase and green fluorescence images were acquired per well from two technical replicates at 20X using the Cytation 5 cell imaging reader (Biotek). Images were acquired immediately after washing and every hour for four hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated for 72 hrs at 37°C in a humidified atmosphere of 5% CO2 before 10 μl of CellTiter-Glo reagent was added to the wells with the MultiFlo Microplate Dispenser (BioTek). Cells were incubated at room temperature for a minimum of 10 minutes after which luminescence readings were recorded by an Infinite M1000 Microplate Reader (Tecan) ...
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bioRxiv - Biophysics 2022Quote: ... the plate was transferred to a prewarmed (37 °C) and 5 % CO2 Cytation V plate reader (BioTeK, Winooski, United States) and incubated for another 10 mins ...
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bioRxiv - Cell Biology 2023Quote: ... at an excitation wavelength of 400 nm and an emission wavelength of 505 nm using the Cytation 5 microplate reader (Biotek). Fold-increase in Cathepsin L activity was determined by comparing the relative fluorescence units (RFU ...
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bioRxiv - Bioengineering 2024Quote: ... Luminescence intensity from the opaque 384-well plate was measured using a microtiter well plate reader (BioTek® Cytation 5).
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bioRxiv - Neuroscience 2024Quote: Microphotographs were obtained with the cell imaging multi-mode plate reader Cytation™ 5 (BioTek Instruments Inc., Winooski, VT, USA) using a green fluorescent protein (GFP ...
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bioRxiv - Cell Biology 2024Quote: ... The MTT crystal were solubilized overnight in solubilization solution (10% SDS in 0.01N HCl) and absorbance was measured and at 570 nm and 660 nm was measured using Cytation 5 plate reader (BioTek) 21.
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed 5 times using ice-cold PBS and fluorescence measurements were recorded using the Synergy H1 microplate reader (Biotek).
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bioRxiv - Immunology 2023Quote: ... and the fluorescence intensity was measured for each well at 560nm absorbance and 590nm emission using Cytation 5 Plate reader from Biotek. For TcdA assays ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The exposure lasted for 23 h before cells were lysed and quantification of luminescence was done on Cytation 5 cell imaging multimode reader (BioTek).
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bioRxiv - Cell Biology 2024Quote: ... The plates were further incubated for 5 minutes at room temperature before the BRET signal was read on a Synergy Neo (Biotek) equipped with dual photomultiplier tubes (PMTs ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence in the upper chamber was measured at 499ex/520em after 72 hours using the Cytation 5 Multi-Mode Reader (BioTek).
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bioRxiv - Bioengineering 2023Quote: ... The absorbance of collected media at 278 nm was measured using a microplate reader Cytation 5 (BioTek Instruments, Winooski, VT) according to the pre-determined standard curve.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Two images per field (NucBlue and Alexa Fluor) and two fields per well were captured on a Cytation 5 cell imaging multimode reader (BioTek) (details in supporting methods and materials) ...
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bioRxiv - Immunology 2022Quote: ... The assay was performed for the next 7 days and each day absorbance was read at 570 nm using Cytation 5 Cell Imaging Multi-Mode Reader (Agilent, BioTek). The assay was performed in triplicate.
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bioRxiv - Microbiology 2022Quote: ... Cell counts in each well were then determined with whole-well fluorescent imaging using a Cytation 5 Cell Imaging Multi-Mode Reader and Gen5 software (BioTek). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: ... We found that we can remove the opaque substances by photobleaching a 5% Spectracide solution (in LB) in a Synergy H1 plate reader (Biotek), at 30°C and 800 r.p.m ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance was read at 450 nm (reference at 620 nm) using a Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Biotek).
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance at 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance of 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Cytotoxicity was evaluated with PrestoBlue Viability Reagent by incubating with cells at 37 °C for 15 minutes and reading fluorescence with excitation 550nm and emission 600nm using a Cytation 5 Plate Reader (BioTek). Luciferase activity was then measured with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were placed on a plate shaker for 30 minutes at room temperature before the luminescence read on a Cytation 5 Imaging Plate Reader (BioTek).
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bioRxiv - Biophysics 2023Quote: ... The bottom of the attractant reservoir was imaged for migrated cells for 8 h with a Cytation 5 BioSpa (Biotek) at 37 °C with 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... One measure of fluorescence (Ex/Em 560/590 nm) was then read each hour (h) using a Cytation 5 (BioTek) driven by the Gene 5.1.11 Software ...
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bioRxiv - Neuroscience 2024Quote: ... The total RNA concentration was measured using an Eon absorbance microplate reader and Gen 5 software (BioTek, Winooski, VT, USA). The RNA was reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Enzyme activity was monitored by measuring the reduction of NADPH at 340 nm using a plate reader (BioTek, Cytation 5) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Levels of eGFP in fibroblast cultures infected with recombinant HCMV reporter viruses were measured using a BioTek Cytation 5 (BioTek). High passage HCMV strain Towne was also propagated as described above.
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bioRxiv - Microbiology 2024Quote: ... The OD600 of the samples and dasherGFP abundance (485 ex/528 em) were then measured using a Cytation 5 (Biotek). The transcriptional activity of the rmp promoter was calculated by dividing the dasherGFP fluorescence intensity by the OD600 for each sample.
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bioRxiv - Neuroscience 2024Quote: ... Fluorescence measurements (Ex/Em 535nm/587nm) were made for 30 minutes on a Cytation 5 plate reader (BioTek, Winooski, VT) with PMT gain set at 50 and a read interval of 2 minutes.
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 5 minutes and proteins were quantified using tryptophan fluorescence in a Synergy H1M microplate reader (BioTek) with an excitation wavelength of 280 nm and an emission wavelength of 360 nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... OD600 readings were recorded every 5 minutes for 30 hours at 30 °C using a microplate reader (Biotek, SYNERGY H1), which included a 10-second shake before each measurement ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence was read (excitation of 440 and 490 nm, both with emission at 535 nm) on a Cytation 5 (BioTek) plate reader incubated at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Molecular Biology 2021Quote: ... and wells were washed three times with 300 μl of diluted washing solution (soak time > 5 seconds) using an automated plate washer (BioTek 50TS). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensities of the acidic solutions were measured at an excitation/emission of 443/505 nm on A Cytation 5 multimode plate reader (BioTek, Inc). Next ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... and infected cells were maintained at 37°C for 14 to 16 h post infection before manual counting of eGFP+ and mNG+ cells or automated counting using a Cytation 5 cell imaging multi-mode reader (BioTek Instruments) and a CellInsight CX5 imager (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with 2.5μM CellROX for 30min at 37°C after which culture media was replaced for imaging on a BioTek Cytation 5 imaging reader (BioTek, VT). Images were captured from 6 random areas of each well every 10min for 2h ...
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bioRxiv - Plant Biology 2021Quote: ... Absorbance of 50 μl aliquots was determined at 648 and 665 nm using a Cytation 5 plate reader (BioTek, Winooski, VT) and content of chlorophyll a and b per μg wet weight determined using the equations in Barnes et al ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and RFP-channel images were taken every 30 minutes for 12 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). Fluorescence quantifications and the counting of fraction of dead worms were performed by a Cell Profiler program ...
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bioRxiv - Cancer Biology 2022Quote: Cancer cell abundance was observed throughout treatment by imaging mono-cultures and co-culture spheroids using a Cytation 5 cell imaging multimode reader (BioTek Instruments) under dual Bright Field and YFP (ex 500 / em 542 for Venus florescence ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated at room temperature for 10 minutes and read using the Cytation 5 96 well plate reader (BioTek, Vermont) and Gen5 software (version 2.07.17 ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...