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Citations for Biotek :
1251 - 1300 of 1433 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Transfer 100 µL of the solubilized crystal violet to another plate and quantify the absorbance at 550 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek.
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bioRxiv - Biochemistry 2020Quote: ... The spectra were recorded using Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek and were averaged by 3 scans.
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 1 hour at 37°C with 5% CO2 and OD440nm & OD650nm were then measured using a 96-well plate spectrophotometer (Molecular Devices or BioTek). OD650nm was subtracted from OD440nm then percent viability was calculated relative to the no treatment condition.
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bioRxiv - Microbiology 2021Quote: ... coli cells suspensions were adjusted to an optical density at 600 nm (OD600; measured using a UV-visible spectrophotometer, Cytation 5, Biotek Inc.) measurement of 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 5 times with S Basal then liquid was aspirated using an EL 406 Plate washer / Liquid dispenser (BioTek, VT). 50 μL of 0.01% tween was added to each well to limit worms adhering to pipette tips ...
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bioRxiv - Microbiology 2022Quote: ... Plate was read for luminescence end-point kinetics with a 1s integration on a Cytation 5 Plate Reader using Gen5 software (Biotek Instruments). Relative luminescence units for each siRNA condition were normalized to NTC siRNA and displayed as a percentage.
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 95 °C for 5 min before the absorbance was measured at 540 nm using a microplate spectrophotometer (BioTek HT).
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bioRxiv - Physiology 2022Quote: ... three representative images of the samples were taken with a Cytation 5 cell imaging multi-mode reader with Gen5 software (Version 3.10; Biotek, Vermont, USA) and analyzed using ImageJ ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of Renilla luciferase activity by adding Stop & Glo® were performed according to the manufacturer instructions using a luminometer reading (Cytation 5 imaging multimode reader, Biotek).
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bioRxiv - Bioengineering 2023Quote: ... the fluorescence of each well was measured at 560/590 nm (excitation/emission) using a microplate reader Cytation 5 (BioTek Instruments). The number of viable cells per well was calculated against a standard curve prepared by plating various concentrations of isolated cells ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... and the resulting emulsion was collected within an Eppendorf tube or the microplate analyzed under the inverted microscope Cytation 5 (BioTek, USA). The isolation of aqueous phase containing RNP-EV from the oil phase was performed under the centrifuge at 2000-3000xg for 5-10min at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Alexa 488 fluorescence (485/20, 528/20 nm) or Alexa 647 (635/25, 670/25) was measured by the plate reader Cytation 5 (BioTek, USA).
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bioRxiv - Biochemistry 2024Quote: PLpro enzyme titration and initial velocity analyses were performed using a Cytation 5 multi-mode microplate reader (BIOTEK Instruments, Winooski, VT) as described previously 15 ...
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bioRxiv - Biophysics 2024Quote: ... λex 320 nm) for each reaction was recorded every 20 seconds on a Cytation 5 plate reader (Biotek, software Gen v3.04). The fluorescence data over time were fitted to the monoexponential decay using the curve_fit function in Scipy Python package: ...
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bioRxiv - Biochemistry 2024Quote: ... detectable light signals in the 520 to 620 nm range were produced and captured with a Cytation 5 plate reader (BioTek®) with the gain value set at 180 ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Cell Biology 2020Quote: ... values were normalized to the cell count using Hoechst dye in the final prot injection and transferred to the Cytation 5 (BioTek, Winooski, VT).
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bioRxiv - Bioengineering 2020Quote: ... Wells were imaged at time of scratch and 24□h and 48 h later using Gen 5 software (BioTek Instruments, Winooski, VT) on a Cytation 5 plate reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... COX activity was determined as the maximal rate of oxidation of fully reduced cytochrome c measured by the change in absorbance over time at 550 nm using an imaging multi-mode reader (BioTek, Cytation 5) at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was quantified by spectrophotometry using a TAKE3 plate in a Cytation 5 multi-mode plate reader (Biotek, Winooski, VT, USA), and RNA quality was assessed by analysis of ribosomal RNA bands visualized following gel electrophoresis by SYBR-safe dye incorporation ...
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bioRxiv - Biochemistry 2021Quote: ... Daily cell number was determined by addition of 20 μl of AqueousOne Solution and measurement of absorbance at 490 nm using a Cytation 5 imaging system (BioTek, Winooski, VT). Fold change was calculated by dividing by absorbance measurements taken at day zero.
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bioRxiv - Microbiology 2021Quote: ... the polymerase activity was monitored by the detection of fluorescence reporter expression on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Sursee, Switzerland) equipped with a 4x air objective (numerical aperture (NA) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ul of the reaction mixture was added to 200 ul of Stop solution and fluorescence was read using Cytation 5 (Biotek, Winooski, VA) at 360 nm (Excitation ...
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bioRxiv - Microbiology 2021Quote: For the expression level determination of the strains with IPTG‐induced folA‐gfp expression (Fig. 5) we used a combination of plate readers (Biotek Synergy H1) for optical density measurements for growth rate determination (see section growth rate measurements for details ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative luciferase units (RLUs) in each well were detected using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2021Quote: ... The growth and lysis kinetics were recorded by measuring the optical density at a wavelength of 600nm (OD600nm) at 37°C with 5-s shaking every 20 min using a 96-well plate reader (Synergy; BioTek, Winooski, VT)
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 3M sulfuric acid 5-10 minutes after OPD addition and absorbance values were measured at a wavelength of 492nm on a spectrophotometer (Biotek Cytation5 system). Absorbance values were normalized by subtracting uncoated wells from antigen-coated wells at the corresponding serum dilution ...
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bioRxiv - Microbiology 2021Quote: ... Quantification was performed by measuring soluble A600 CV absorbance using a Biotek Synergy HT microplate reader and Gen 5 software (Biotek, Winooski, VT). Values were normalized to culture growth by dividing by final culture OD600.
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bioRxiv - Biochemistry 2022Quote: Cy5.5-ALN was characterized in terms of absorbance in the NIR region using a plate reader (Cytation 5, BioTek, Winooski, VT, USA). Yield was calculated using ε675nm=211000 M/cm ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were maintained at 5% CO2 and 37°C during imaging using Cytation Gen5 Microplate Reader and Imager Software (BioTek, version 3.08.01). Protocols specific to Falcon 24 well companion plates (Corning ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by adding 50 μl of stop solution and the absorbance was recorded at 450nm using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: ... Plasmin activity was monitored by continuously measuring the rate of change of absorbance at 405 nm for 20 minutes using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Bioengineering 2023Quote: ... was quantified by measuring the absorption at 620 nm using a UV/Vis-spectrophotometer (Cytation 5 imaging reader: BioTek, Winooski, VT, USA). A standard curve was used to correlate the absorption value with the amount of HA ...
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bioRxiv - Microbiology 2023Quote: ... 2N H2SO4 solution was added to stop the reaction and absorbance was measured at 450 nm using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: GFP or mCherry-positive cells were imaged on the Cytation 5 imaging reader and masked using the accompanying Gen5 software (BioTek Instruments, Inc.). The final cell-to-cell spread was calculated as GFP or mCherry-positive cells per area of the entire well ...
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bioRxiv - Immunology 2024Quote: ... Peptide loading was determined by fluorescence quantification using a modified fluorescamine peptide quantification assay in the presence of ACN (Ex/Em: 390/465 nm, Biotek Cytation 5)104.
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bioRxiv - Bioengineering 2024Quote: ... and absorbance at 492 nm was monitored in one-minute kinetic interval cycles for 30 minutes using a plate reader (Biotek Cytation 5). The experiment was repeated for the samples after fabrication and biweekly upon storage in physiological conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... COX activity was determined as the maximal rate of oxidation of fully reduced cytochrome c measured by the change in absorbance over time at 550 nm using an imaging multi-mode reader (BioTek, Cytation 5) at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...
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bioRxiv - Biochemistry 2024Quote: The polymerization and depolymerization of pyrene–labeled recombinant WT or mutant actin (5% pyrene-labeled WT or mutant actin) was monitored as a function of increasing or decreasing pyrene– fluorescence using a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) and the built–in filter set (Excitation ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 4% PFA and stained with Hoechst nuclear stain before GFP-positive cells were counted using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) with the onboard software for calculations of percentage infected cells.
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then maintained at 37°C for 14-16 h post infection before automatic counting of GFP-positive cells using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) and using the onboard software for calculations of percentage infected cells.
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bioRxiv - Cancer Biology 2021Quote: ... at 1.5×104 cells/ well in 50 μl of DMEM supplemented with 10% FBS using BioTek™ MultiFlo™ FX Microplate Dispensers (BioTek Instruments). Cells were treated with an FDA approved library (Selleckchem ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was quantified using the Take3 microvolume plate accessory on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States), RNA purity was determined by analyzing A260/280 and A260/230 ratios ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescence intensity was measured at the vascular bundles of leaves 5 mm from the detachment sight using the Lionheart FX Gen5 image analysis mode (BioTek, Winnoski, VT, USA). Untreated plants were used as control with 6 biological repeats ...
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...