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Citations for Biotek :
601 - 650 of 868 citations for Recombinant Human H1 Histone Family Member 0 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Measurements were taken at 340 nm based on the decrease of NADH absorbance over the course of one hour on SYNERGY H1 microplate reader (BioTek). Statistical analysis was performed using GraphPad Prism 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 12000 rpm for 5 minutes and the absorbance of supernatants was read at 475nm in the Synergy-H1 microplate reader (BioTek). Absorbance values were compared to the standard curve of synthetic melanin (155343 ...
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bioRxiv - Microbiology 2020Quote: ... The RFU (Relative Fluorescent Unit) of the RFP data was collected using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek) in fluorescence mode ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial growth was monitored over time in triplicates by measuring the optical density at 600 nm (OD600) using a microplate reader (Synergy H1 or Cytation5 Hybrid Multi-Mode Reader, BioTek). L ...
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bioRxiv - Microbiology 2021Quote: ... samples were transferred to fully dark 96 well plates and fluorescence was read in a Synergy H1 Hybrid Multi-Mode Reader (BioTek) with excitation at 440 nm and reading at 485 nm ...
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bioRxiv - Microbiology 2020Quote: ... as substrate which is dephosphorylated by the ALP enzyme forming p-nitrophenol which was measured spectrophotometrically at 405 nm in Synergy H1 Hybrid Reader (BioTek) [16] ...
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bioRxiv - Cancer Biology 2020Quote: ... Fluorescence was expressed as H2O2 released using a standard curve generated with known concentrations of H2O2 stabilized solution using the Synergy H1 Hybrid microplate reader from BioTek at excitation of 530 nm and emission of 590 nm ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of the supernatant was removed and analyzed to determine the amount of FITC-labeled IgG using a Synergy H1 Hybrid Multi-Mode Plate Reader (BioTek). Release assays were conducted by adding 100 μL hydrogel to microcentrifuge tubes and then centrifuging until the hydrogel was situated in the bottom of the tube ...
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bioRxiv - Microbiology 2022Quote: ... The plates were further incubated for six hours at 37°C in a 5% CO2 atmosphere and then analyzed spectrophotometrically (Synergy H1, Biotek) at 640 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... and ThioT fluorescence (λEx=440 nm, λEm=485nm) was recorded at 37°C in a plate reader (Synergy H1, Biotek) every 15 min after a 5 s shake.
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bioRxiv - Microbiology 2022Quote: ... Plates were read for absorbance at 610 nm on a Synergy H1 plate reader (BioTek Instruments, Inc., Winooski, VT, USA).
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bioRxiv - Bioengineering 2022Quote: ... PGT121 concentration was measured by absorbance intensity at 450 nm using a Synergy H1 Hybrid Multi-Mode Plate Reader (BioTek). Each plate contained a 15-point standard curve assayed in duplicate which was used to interpolate PGT121 concentrations by fitting a four-parameter dose-response curve (variable slope ...
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bioRxiv - Cancer Biology 2022Quote: ... The mixture containing 50 µl of the protein supernatant and 50 µl of PBS was subjected to the measurement of emission and excitation spectrum by the microplate reader (Synergy H1, BioTek).
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bioRxiv - Molecular Biology 2021Quote: ... Infection rate by pseudoviruses were determined by fluorescence or luminescence reads in a microplate reader (BioTek’s Synergy H1, VT, USA), as GFP or fLuc-expressing reporter plasmids were packaged during pseudovirus production ...
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bioRxiv - Cell Biology 2021Quote: ... PBS was replaced at 100μl per well before readout at 490nm excitation and 525nm emission (fixed gain of 150) on a Synergy H1 plate reader (Biotek). Conditioned media from at least 2 independent experiments per cell line were analyzed and shown.
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bioRxiv - Microbiology 2020Quote: ... Growth was monitored by measuring every 5 minutes the optical density of liquid cultures cultivated in 96 well plates at a wavelength of 600 nm using Cytation3 or Synergy H1 plate readers (Biotek).
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bioRxiv - Microbiology 2021Quote: ... was added to the cells and incubated in the dark for 15 min before reading luminescence with Synergy H1 microplate reader (BioTek). Measurements were done in duplicate and relative luciferase units were converted to percent neutralization and plotted with a non-linear regression model to determine IC50 values using GraphPad PRISM software (version 9.0.0).
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bioRxiv - Molecular Biology 2020Quote: ... The ratio of pHluorin emission fluorescence intensity at 520 nm produced by excitation at 395 nm and 480 nm was measured using a Synergy H1 hybrid fluorescence plate reader (Biotek). For intracellular pH measurements ...
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bioRxiv - Microbiology 2021Quote: ... 65 µL of fluorescent dye were added and fluorescence was determined on a per-well basis in a Synergy H1 multi-mode plate reader (BioTek). 0% transcriptional activity was defined by the no-enzyme control and 100% activity was defined by an untreated control.
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 50µL of 3M hydrochloric acid and then absorbance (at 490nm) was immediately determined using Synergy H1 hybrid multimode microplate reader (BioTek). PRISM 7.0 was used to determine the effective concentration of each virus that would yield detectable NA activity ...
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bioRxiv - Microbiology 2021Quote: ... 37 and 43°C were performed in 96-well plates each containing 200 μl of LB and cell density (OD 600) was measured with a Synergy H1 plate reader (Biotek). Spot assays were performed by spotting 10 μl of serial dilutions of cultures (at initial OD600 of 1.0 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The reactions were next transferred to a 384 conical well plate and incubated in the Synergy H1 plate reader (BioTek) at 30 °C for 3-4 h ...
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bioRxiv - Microbiology 2022Quote: ... the seal was removed to expose cells to oxygen and then the plates were read in a Synergy H1 (BioTek) plate reader under ambient conditions at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed in 96-well-plates and absorbance at 570 nm and 600 nm were measured by the Synergy H1 Hybrid microplate reader (BioTek) overnight ...
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bioRxiv - Microbiology 2022Quote: ... and sulfuric acid and quantified at 450 nm using a BioTek Synergy H1 plate reader (BioTek Instruments Inc., Winoski, VT). Values were analyzed in Prism 9.0 (GraphPad ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed twice with PBS and stained biofilms were resuspended in 7% acetic acid (v/v) and absorbance was read at 595 nm in a Synergy H1 hybrid multimode reader (BioTek).
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bioRxiv - Synthetic Biology 2020Quote: ... which was incubated at 37° C for 12 h with double-orbital shaking in a Synergy H1 microplate reader (Biotek). Cells were then diluted 1:120 in 120 µl of selective EZ containing the opposed inducer (10 µM AHL or 0.2% Ara ...
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bioRxiv - Biochemistry 2019Quote: ... in PBS was added to 10 µl of cell cultured medium and the luminescence was measured on Synergy H1 (BioTek) microplate reader.
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bioRxiv - Microbiology 2019Quote: ... Fluorescence (excitation: 557nm, emission: 592nm) and OD600 were monitored during incubation in a microplate reader (Synergy H1, BioTek Instruments, VT) with continuous ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and cDNA quality and quantity were checked using Synergy™ H1 Multimode Microplate Reader (BioTek Instruments, Inc., VT, USA). qPCR analysis was performed using iTaq Universal SYBR Green Supermix (BIO-RAD ...
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bioRxiv - Molecular Biology 2020Quote: ... the absorbance of each sample at 670 nm was measured in a microplate reader (Synergy H1 Multi-Mode Reader-Biotek) so as to calculate the amount of product.
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bioRxiv - Synthetic Biology 2021Quote: ... or at an excitation wavelength of 472 nm and emission wavelength of 507 nm for 3WJdB-activated fluorescence on a plate reader (Synergy H1, BioTek). Fluorescence values for a fluorescein concentration in which a single replicate saturated the plate reader were excluded from analysis ...
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bioRxiv - Genetics 2021Quote: ... Nile Red fluorescence (excitation at 488 nm; emission at 565 nm) was measured in a multi-well plate reader (Synergy H1, Biotek), normalized to cell density (determined as absorbance at 750 nm ...
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bioRxiv - Microbiology 2019Quote: ... Listeria cells were incubated at 30°C and Pseudomonas at 37°C with continuous double-orbital rotation for 16-48 hr in the Synergy H1 Hybrid Multi-Mode Reader (BioTeK) and measurements of OD600 and mCherry (excitation 570 nm ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C with continuous fast linear shaking at 567 cycles per minute (cpm) in a microplate reader (Synergy H1; BioTek). OD600 and fluorescence readings were taken every hour over a 24-hour period as previously described (85) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C with continuous fast linear shaking at 567 cycles per minute (cpm) in a microplate reader (Synergy H1; BioTek). OD600 and fluorescence (mCherry and eGFP were detected at 580 to 610 λm and 488 to 509 λm ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored at an optical density at 600 nm (OD600) using either a spectrophotometer or a 96-well microtiter plate reader (Synergy H1; BioTek).
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bioRxiv - Microbiology 2020Quote: ... plates were washed and crystal violet dissolved in 70% ethanol at room temperature and absorbance measured at 595 nm with a microplate reader (Synergy H1; BioTek). Data analysis was performed to calculate the mean and standard deviation ...
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bioRxiv - Microbiology 2021Quote: ... were transferred to white 96-well plates with clear bottom and the optical density (OD600nm) and the luminescence of the samples were measured using the plate reader Synergy H1 (BioTek). The integration time for luminescence detection was of 5 seconds ...
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bioRxiv - Microbiology 2021Quote: ... The resuspended samples were transferred to black 96-well plates and the fluorescence was measured using the plate reader Synergy H1 (BioTek). The excitation and emission settings for the detection of Amcyan and TurboRFP were 420/520 nm and 550/580 nm respectively ...
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bioRxiv - Microbiology 2019Quote: ... Absorbance values were measured at 450 nm with wavelength correction of 570 nm using a microplate reader (Synergy H1, BioTek). To confirm that bacterial lysis had not occurred during sample processing and plate coating ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence levels were recorded every 5 minutes over a period of 500 minutes using a Synergy H1 hybrid reader system (Biotek) at excitation/emission wavelengths of 488/515 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... The amount of FITC-dextran in the serum was determined by measuring the fluorescence at an excitation wavelength of 485 nm and an emission wavelength of 525 nm using a plate reader (Synergy microplate reader, H1 Biotek).
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bioRxiv - Plant Biology 2019Quote: ... and 645 nm and 663 nm for total chlorophyll by using a microplate reader (Synergy H1 Hybrid Reader; Biotek, Korea).
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bioRxiv - Biochemistry 2020Quote: ... in PBS was added to 10 μL cell culture medium and the luminescence was measured on a Synergy H1 (BioTek) microplate reader ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then solubilised in 2 %w/v SDS/PBS and absorbance measured at 595 nm using Synergy H1 microplate reader (BioTek). Statistical analysis was performed at the endpoint across repeats ...
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bioRxiv - Microbiology 2021Quote: ... samples were transferred to a clear 96-well plate and OD (405 nm) was measured for the entire well using the well area scan feature of the Synergy H1 microplate reader (BioTek). The average OD405 nm for each well was blanked against duplicate thrombin-free controls for each experimental sample type.
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bioRxiv - Biophysics 2020Quote: ... followed by measuring the absorbance at 280 nm and fluorescence at 525 nm (excitation = 498 nm) on a multi-mode microplate reader (Synergy H1, BioTek).
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bioRxiv - Evolutionary Biology 2021Quote: ... Baseline fluorescence intensity was measured with excitation at 350 nm and emission at 435 nm and 500 nm for 5 min at 37°C on a Synergy H1 spectrophotometer (BioTek). Cells were then treated with 10% ethanol as a membrane fluidizer ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C with continuous orbital shaking at 807 cycles per minute (cpm) in a Synergy H1 microplate reader (BioTek) with fluorescence and optical density measurements every 30 minutes (mScarlet ...