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Citations for R&D Systems :
351 - 400 of 3432 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... and 100 ng/ml CHO-derived mouse R-spondin 1 or 5 ng/ml CHO-derived mouse R-spondin 2 (R&D System). The final concentration of Rspondins in the culture medium was initially tested in pilot experiments in order to add comparable amounts of bioactive ligands (bioactivity measured by TOPflash assays and organoid growth curves ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3) were purchased from R&D Systems.
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bioRxiv - Cancer Biology 2022Quote: ... human interleukin-3 (IL-3, 10 ng/mL, R&D Systems), human interleukin-6 (IL-6 ...
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bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
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bioRxiv - Cell Biology 2019Quote: ... prior to 1 hour incubation of CD206 (1:200, R&D systems, AF2535), F480 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL neuregulin-1 (recombinant human NRG-1; R&D Systems)) at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-βIII-Tubulin mAB (clone TuJ-1, 1:1000, R&D Systems MAB1195). The membranes were incubated with the primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD31/PECAM-1 (dilution 1:300, goat polyclonal, AF3628 R&D Systems) + anti-goat AF546 (dilution 1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal anti-goat TIMP-1 (R&D Systems; Minneapolis, MN; 1:300 dilution), monoclonal anti-mouse microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2019Quote: ... and Mfge8 (Thermo MA5-23913, 1:50; R&D Systems MAB2805, 1:50). All Ltf and Mfge8 antibodies have been previously validated by staining lactating mammary glands.
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bioRxiv - Cell Biology 2021Quote: ... IGF-1 (mouse/rat IGF-1 ELISA kit, R&D system, Minneapolis, MN) and total thyroxine (total thyroxine ELISA ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies Nkx6.1 (1:100, DSHB) and Pdx1 (1:100, R&D Systems) were added and incubated for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... CINC-1 and TIMP-1 were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Neuroscience 2023Quote: ... recombinant murine TIMP-1 (rmTIMP-1, R&D Systems, Minneapolis, MN; 10ng/mL) was added 24 hours prior to harvesting of protein/RNA.
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bioRxiv - Neuroscience 2023Quote: Exogenous IGF-1 Administration: Human recombinant IGF-1 (R&D Systems 291-G1) was reconstituted in sterile saline and 5µls (30µgSE ...
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bioRxiv - Bioengineering 2023Quote: ... and goat anti-jagged 1 (1:150, AF599, R&D Systems, RRID: AB_2128257). Secondary antibodies (all raised in donkey ...
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bioRxiv - Neuroscience 2023Quote: ... TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit, R&D Systems, catalog # MTM100) and MCP-1 (Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293; anti-PTDGS/PGD2 R&D Systems CAT#MAB10099 1:100 ...
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... was measured immediately before injection and at 3 days after injection using a Mouse Lipocalin-2/NGAL Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems) to ensure successful injection of folic acid ...
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human interleukin 4 (IL-4) (R&D Systems), Alpha MEM with nucleosides (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 2ng/mL rhIL-7 and 0.4ng/mL rhIL-15 (R&D Systems). T cells were transduced 24 hours post-activation with lentivirus encoding an anti-EGFR CAR with 41bb/CD3ζ ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems). For culture ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
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bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-AXL (AF154, IF 1:500, WB 1:1000) from R&D Systems; mouse anti-AKT (2920 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-Sox2 (SC17320, Santa Cruz Biotechnology; 1:300; MAB2018, R&D Systems 1:50), anti-Sox9 (AF3075 ...
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bioRxiv - Biochemistry 2022Quote: ... RhoA (1:500, NBP2-22529 NovusBio) and Klotho (1:1000, AF1819 R&D Systems). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase-conjugated secondary antibodies (Milian Analytica ...
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bioRxiv - Cancer Biology 2021Quote: ... CCL27 (1:200, HIER 1, mouse monoclonal, clone 124308, R&D Systems, USA; MAB376), CCR10 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Mouse sFRP-1 Protein (1 µg/ml, R&D Systems, MN9019-SF-025), Plexin-D1 Fc fragment (30 µg/ml) ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor (Thermo 1:200) and Northern Lights (R&D Systems 1:200) conjugated secondary antibodies were incubated for 1 hour at 37ºC ...
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bioRxiv - Physiology 2023Quote: ... and Goat anti mouse CD31/PECAM-1 (AF3628-SP, 1:150, R&D Systems) diluted in blocking buffer ...