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Citations for R&D Systems :
151 - 200 of 3432 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Neuroscience 2020Quote: ... Myeloid progenitor cells were treated in suspension for 1 h with 5μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920 ...
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 3 and 6 days and subsequently tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... and homogenates were diluted to 2 mg/mL protein in PBS and were subsequently diluted 1:2 in Reagent Diluent (R&D Systems). CXCL10 protein was quantified using the Mouse CXCL10 Duoset ELISA kit (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 1 ng/mL transforming growth factor beta-1 (TGF-β1) and 5 ng/mL fibroblast growth factor-2 (FGF-2) (both from R&D Systems) at 37°C and 5% CO2 in a humidified incubator (Thermo Scientific Heraeus ...
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Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 24 and 48 hours intervals from 3 BALB/C mice carrying RCC tumours receiving 1×107 PODS-IL-2 treatment following the final dose and tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody was added for ULBP 1 and 2/5/6 only (R&D Systems). Cells were then washed and fixed in 4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were labeled with anti-human SSEA-4 APC-conjugated antibody (1:100, R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:200; Af114, R&D Systems). The next day ...
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Cell Biology 2023Quote: ... 400 µg of protein from mock- and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... ELISA for IL-7 was performed using Mouse IL-7 Quantikine ELISA Kit M700 (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... PlGF-2 (3 – 30 ng; 5 µl; cat.465-PL/CF, R&D System, USA), VEGF-E (3 – 30 ng ...
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bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Neuroscience 2019Quote: ... and BSA (2%) and stained with following primary antibodies: PI16 (1:75, R&D Systems AF4929), GLUT1 (1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-MAP2 (aka Microtubule-Associated Protein 2; 1:250; R&D Systems, Cat #: MAB8304, RRID:AB_2814693), rabbit anti-Myosin7 (MYO7 ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human SUMO-1 and SUMO-2 were purchased from R&D systems (Cat. # K-700). Recombinant human SUMO activating enzyme E1 (SAE1/UBA2 ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-rabbit Nucleocapsid (NovusBiologics ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-mouse H3 (Immune-Tech ...
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were incubated overnight at 4°C with anti-Gremlin1 antibody (R&D systems, AF956, 1:1000) or anti-βActin (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were incubated overnight at 4 °C with goat anti Dscam (AF3666, 1:500, R&D systems) and mouse anti-tubulin (12G10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C with primary antibodies against pLCK (Y394) (1:1000) (R&D Systems), GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight at 4°C with antibodies against ACE2 (1:400; R&D systems, Minneapolis, MN, USA), TMPRSS2 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell pellet was stained for 1 hour at 4°C with primary ACE2 antibody (R&D systems #AF933 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Cell Biology 2019Quote: ... secreted IL-2 samples were diluted 1:10 and subsequently analyzed using Mouse IL-2 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN), as per manufacturer’s specifications.
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bioRxiv - Bioengineering 2023Quote: ... IL-7 (5ng/ml; R&D Systems) and IL-15 (5ng/ml ...
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bioRxiv - Bioengineering 2020Quote: ... Mouse IL-28 A/B (IFN-lambda 2/3) Du-oSet ELISA (DY1789B, R&D Systems), was used to assess cross-reactivity.
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... cover glasses were coated for 2 h at 37°C with 100 nM recombinant ICAM1-FC (796IC, R&D Systems) and washed 2 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Molecular Biology 2023Quote: ... and Fluorogenic Peptide Substrate VII: Z-Leu-Arg-4-methyl-7-coumarylamide (Z-LR-AMC)(Catalog # ES008) were purchased from R&D Systems (Minneapolis, MN, USA). The enzyme reactions were initiated upon addition of ice-cold of hrCTSL (96pM ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated with recombinant Jagged 1 protein (JAG1, 2 µg/ml, R&D System) for 3 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 1% PS and 100 IU/ml of human recombinant IL-2 (R&D Systems, 202-IL-050). Cells were used at low passage numbers ...