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Citations for R&D Systems :
4451 - 4500 of 4725 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with ice-cold acetone and stained with 1:200 dilution of monoclonal-anti-RBD (R&D Systems, MN, USA), polyclonal-anti-S1- ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with the appropriate primary antibody overnight at 4°C: anti-IL1 (goat, 1:1000, R&D Systems), anti-phosphorylated tau (AT8 ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibody dilutions were made 1:2000 in PBST: goat α-mouse IgG-HRP (R&D Systems; HAF007) and goat α-rabbit IgG-HRP (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted at 1:1000 in TBST 0.1 % supplemented with 2.5 % BSA or with Goat anti- GAPDH antibody (AF5718, R&D systems) diluted at in TBST-0.1 % supplemented with 2.5 % milk or anti-V5 HRP (Thermofisher scientific ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence staining: goat anti-mouse VE-cadherin (R&D Systems, AF1002; 1:200), hamster anti-mouse PDPN (Developmental Studies Hybridoma Bank ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were incubated overnight at 4°C with the primary antibodies for A2B5 (1:50; R&D Systems, MAB1416) and NG2 (1:300 ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... antigen-retrieved in pH6 sodium citrate buffer and stain with primary antibodies including: anti-LYVE-1 (AF2089; R&D System), anti-CXCL8 (MAB208 ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for immunoblotting: Goat IgG against VE-cadherin (1:500, AF1002, R&D Systems, Minneapolis, MN), pY658 VEC (1:250 ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 1:10 and IL8 was measured using the DuoSet IL8 ELISA kit (R&D Systems cat# DY208) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Bioengineering 2024Quote: ... or Epiregulin (EREG) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#1195-EP-025/CF) with Noggin-Fc (100ng/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blotted using the primary antibodies: Ebi3 (2.5 μg/ml; eBioscience) or IL-12/IL-35 p35 (1 μg/ml; R&D Systems), phospho-mTOR (Ser2448 ...
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Neuroscience 2019Quote: Antibodies and dilutions used for immunofluorescence staining were as follows: polyclonal goat anti-mouse Pdgfra (R&D Systems, AF1062, 1:500), monoclonal rabbit anti-mouse Olig2 (Abcam EPR2673 ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were differentiated into macrophages and dendritic cells using 50 ng/ml GM-CSF (R&D Systems, https://www.rndsystems.com) + 50 ng/ml M-CSF (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 min at 4°C) pre-incubated with 5 μl TRKB antibody (Goat anti-mouse TRKB Antibody, #AF1494, R&D Systems) and cleared through new Protein-G sepharose tubes ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Gels were then subjected to immunoblot analysis for CHIKV E using an anti-E antibody (1:1000, R&D Systems, MAB97792SP).
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bioRxiv - Developmental Biology 2022Quote: ... Agents were added to the medium at final concentrations of 1 mg/ml for BMP2 (355-BEC-010, R&D Systems), 500 μM for GdCl3 (16506-71 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged and resuspended in mTeSR1 supplemented with 10 μM SB431542 (SB; Stemgent) and 1 μM Dorsomorphin (R&D Systems). 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm ...
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bioRxiv - Cancer Biology 2021Quote: Cells were released from plates by incubating with 0.05% trypsin and then stained with primary antibody (1 μg/106 live cells) against NPY1R (MAB6400; R&D Systems) followed by secondary antibody against human IgG for 30 minutes on ice and/or with PE-conjugated ABCC5 (sc-376965 PE ...
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bioRxiv - Neuroscience 2022Quote: ... Group 1 ligands were derived from mouse transcriptomic data and included: APP (R&D Systems, Cat. 3466-PI-010, 20ng/mL), APOE3 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated in primary antibody (chicken anti-GFP, Aves labs GFP-1010, 1/500; goat anti-PDGFRA, R&D Systems AF1062 ...
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bioRxiv - Immunology 2022Quote: ... then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies: CD4 (1:300; clone GK1.5; R&D Systems); CD44 (1:300 ...
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bioRxiv - Microbiology 2019Quote: ... using known concentrations of commercial rBoIFN-γ (20, 15, 10, 5, 3 and 1 ng; 2300-BG-025, R&D Systems).
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bioRxiv - Neuroscience 2020Quote: ... OPCs were treated with LCN2 (1, 2.5, 5, 10, 20, 40, 80, or 160 ng/ml, R&D Systems, Shanghai, China) or vehicle control (0.0002% BSA ...
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bioRxiv - Immunology 2020Quote: ... Plasma was assayed in duplicate for CCL2 using the same CCL2/JE/MCP-1 DuoSet ELISA kit (R&D Systems™) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were utilized for immunoblots: goat anti-CD31 antibody (1:500, #AF3628, R&D Systems, Minneapolis, MN, USA) for mouse ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) B27 Serum-Free Supplement (50x) (Life Technology, 17504044) and 50 ng/ml FGF7 (R&D System, 251-KG). For posterior foregut stage (d7-d8) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against myeloperoxidase (1:150 dilution; R&D Systems, AF3667), citrullinated histone H3 antibody (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... Sanofi-aventis Cat# NAC2004–5843-01) and interleukin-4 (IL-4; 1000 IU/mL; R&D Systems Cat# 204-1 L). Mature ...
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bioRxiv - Immunology 2020Quote: Recombinant mouse IL-27 (<1.0 EU per 1 μg of the rmIL-27 protein by the LAL method) was purchased from R&D systems.
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from CH12 cells cultured for 3 days with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Myeloid progenitor cells were treated in suspension for 1 h with 5μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fixed cells were stained with 1:100 dilution of APC-conjugated anti-SOX-17 (R&D Systems, Minneapolis, MN, USA; IC1924A) for 30 mins at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).