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Citations for R&D Systems :
4401 - 4450 of 4725 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Gradients were generated in the Dunn chamber with 200 ng/ml Netrin-1 (R&D Systems, # 1109-N1-025) in the outer well ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and incubated with an anti-mouse HRP-conjugated secondary antibody (R&D Systems; 1:1000) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Then 1 ml of fresh media was supplemented with IL-34 (R&D systems, cat. No. 5265-IL-010), MCSF (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated to streptavidin (R&D Systems), diluted 40× in T-PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were washed in PBS and coated with 1 µg / mL laminin-I (R&D Systems; #3446-005-01) at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Bioengineering 2023Quote: ... The primary antibodies used in this study include anti-Mouse Myosin Heavy Chain (1:1000, R&D Systems, MAB4470), anti-Human/Mouse/Rat/Chicken Pax7 (5μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used at the indicated dilutions: goat anti-Nurr1 (AF2156, R&D Systems; 1:250), rabbit anti-Nr2f2 (ab211776 ...
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bioRxiv - Neuroscience 2023Quote: ... The neural medium was supplemented with 20 ng ml−1 epidermal growth factor (EGF; R&D Systems, 236-EG) and 20 ng ml−1 basic fibroblast growth factor (FGF2 ...
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bioRxiv - Neuroscience 2024Quote: ... The neural medium was supplemented with epidermal growth factor (EGF, 20 ng ml-1, R&D Systems, 236-EG) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Neuroscience 2023Quote: ... Specified primary antibodies were incubated over-night at 4°C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31 ...
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bioRxiv - Cancer Biology 2023Quote: Western Blotting primary antibodies and working dilutions used were: goat anti-CD276 (1 µg/ml; R&D systems, #AF1027); mouse anti-α-tubulin (1:1’000 ...
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bioRxiv - Microbiology 2024Quote: ... followed by matching horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature (R&D Systems, MN, USA). Chemiluminescent imaging was performed using the LAS-4000 platform and histogram densitometry was performed using Multi Gauge software (V3.1 Fujifilm ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for immunostaining of mice tissues: goat anti-CD31 (1:500; R&D systems), goat anti-LYVE1 (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels for IL-6 and MCP-1 were determined by ELISA according to manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... IL-1β release was measured using the Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit (R&D Systems) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated in permeabilization buffer containing the primary REG3β antibody (1:250 dilution; R&D systems, Minneapolis, MN) overnight (16 h) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies for western blot assays were used at the following concentrations: anti-SP-D (1:2,000, R&D Systems, AF1920), anti-LPO (1:2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... At day 1 the medium was changed to base media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Immunology 2022Quote: ... PE-conjugated anti-CTLA-4 and APC-conjugated anti-CD279 (PD-1) were purchased from R&D System (Minneapolis, MN). Human cytokines from cell culture supernatants were analyzed using ELISA kits for TNF ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone ...
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bioRxiv - Physiology 2020Quote: ... Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... For proximity ligation assays VE-cadherin was detected using mouse VE-cadherin antibody (1:200, R&D systems, Goat, AF1002). c-Src was detected using anti-Src (GD11 clone ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems, UK). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Cancer Biology 2020Quote: ... 8 μm cryosections were fixed in acetone and incubated with goat-anti-mouse CD31 (R&D system, #AF3628, 1:200) and rabbit-anti-FITC (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody information is as following: Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems; AF2335; 1:100), Rat anti-mouse vascular adhesion molecule (VCAM1 ...
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bioRxiv - Microbiology 2021Quote: ... 10ng/ml interleukin 4 (IL-4) and 10ng/ml transforming growth factor beta 1 (TGFβ1) (R&D systems, Minneapolis, MN), and used between days 7-9 of differentiation.
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bioRxiv - Cancer Biology 2021Quote: ... or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200, AF6249, R&D Systems, Minneapolis, MN, USA) in Antibody Diluent Buffer from Ventana Medical Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and dilutions used for immunohistochemistry in this study were the following: PDGFRA (goat, 1:70; R&D Systems); GPNMB (goat ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 and then incubated overnight at 4°C with mouse monoclonal anti-CD154 (1:50, R&D systems, MAB617) and rabbit anti-calbindinD28k (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro ubiquitylation assay reactions in 1 x ubiquitin conjugation reaction buffer (R&D systems Cat. # B-70) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro SUMOylation assay reaction in 1 x SUMO conjugation reaction buffer (R&D systems Cat. # B-60) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Physiology 2020Quote: ... followed by overnight incubation at 4 °C with primary antibodies against UCP1 (1:1000, R&D systems, Minneapolis, MN, USA), 14-3-3ζ (1:1000 Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were applied overnight at 4 °C: goat anti-OTX2 (1:500, R&D Systems, cat# AF1979), mouse anti-CDX2 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with HRP-conjugated IgG Secondary antibody (anti-goat, 1:5000; # HAF017, R&D Systems, goat anti-rabbit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then resuspended in 20 μl of 1× ubiquitin reaction buffer containing 200 nM UBE1 E1 (R&D systems, U-110), 1 μM UbcH5a E2 (Enzo ...
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bioRxiv - Developmental Biology 2021Quote: ... At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Developmental Biology 2022Quote: ... ITGA8 was stained by the use of polyclonal goat anti-Integrin alpha 8 antibody (1:50; R&D Systems, Minneapolis) and GDNF was stained by the use of polyclonal rabbit anti-GDNF antibody (1:100 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The following secondary antibodies were used at a 1:5000 dilution: goat anti-rabbit IgG-HRP (R&D Systems; HAF008) and goat anti-mouse IgG-HRP (R&D Systems ...
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bioRxiv - Biochemistry 2024Quote: ... carboxylesterase 1 (CES1) and trans-epoxy-succinyl-L-leucylamino(4-guanidino)butane (E-64) were purchased from R&D Systems. The respective genes coding for the human recombinant enzymes Histidine triad nucleotide binding protein 1 (HINT1) ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used for our IHC study: Goat anti-Serpina3n (1:3000, R&D System, Cat# AF4709, RRID:AB_2270116); Mouse anti-CC-1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...