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Citations for R&D Systems :
401 - 450 of 478 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and the supernatant was used for the quantification of IL-1β e IL-6 cytokines using commercial kits (R&D System®).
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit, R&D Systems). All media was collected two days following media change ...
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bioRxiv - Pathology 2020Quote: ... recombinant human Cadherin 6 Fc chimera protein (~130 kD) or recombinant mouse Cadherin 6 protein (~90 kD) was used to generate a standard curve (R&D Systems catalog # 2715-CA or 6826-CA ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by ELISA using the DuoSet ELISA Kits (DY210-05, DY201-05, and DY206-05) from R&D Systems per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... or 100 ng/ml N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine (Pam3CSK4) for 6 h and IL6 secretion measured by ELISA (R&D Systems).
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Microbiology 2022Quote: Distal ileal TNF-α and IL-6 were quantified using the mouse TNF-α enzyme-linked immunosorbent assay [ELISA] Ready-SET-Go kit (eBioscience; San Diego, CA, USA) and the Mouse IL-6 DuoSet ELISA (R&D Systems) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Cell Biology 2024Quote: Extracellular conditioned media from differentiated 3T3-L1 cells pretreated with or without palmitate and UNC0642 were quantified using mouse IL-6 Quantikine ELISA kit M6000B from R&D Systems as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were transferred to a new eppendorf tube and used for measuring the levels of cytokines using the Mouse TNF-alpha Quantikine ELISA kit and the Mouse IL-6 Quantikine ELISA kit (R&D systems), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Immunology 2024Quote: ... Th17- (10 ng/mL IL-1β [STEMCELL Technologies], 10 ng/mL IL-6 [STEMCELL Technologies], 10 ng/mL TGFβ [R&D Systems] ...
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bioRxiv - Cancer Biology 2024Quote: ... Kuramochi) was evaluated 24 h after changing the medium using the Human IL-6 DuoSet ELISA Kit (DY206-05, R&D Systems) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: Monocytes were isolated from the bone marrow of C57BL/6 mice and differentiated into macrophages by incubation for seven days in media containing recombinant CSF1 (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
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bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
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bioRxiv - Cell Biology 2024Quote: ... co-housed 8-week-old female C57BL/6J wildtype mice were administered either 300 μg/kg TNF (R&D Systems Cat#410-MT/CF Lot CS152103) in endotoxin-free DPBS or endotoxin-free DPBS alone (Merck Cat# TMS-012-A ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
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bioRxiv - Microbiology 2021Quote: The secreted TNF-α and IL-6 cytokines in the culture supernatants were quantified by ELISA using Duo Set ELISA (R&D systems) as per the manufactures instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... condition media was collected and estimated quantity of the secretary IL-6 using commercially available ELISA kit (R&D Systems, DY 206-05) with human IL6 antibody (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serum levels of cytokines (IL-6, TNF-α, MCP-1 and IL-10) were measured by corresponding ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and hESCs were seeded at 3×105 cells/well onto 6-well plates and rested for 24hrs prior to stimulation with PBS or rhIL-23 (1290-IL-010, R&D Systems, USA) at concentrations of 1 ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Cell Biology 2024Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Molecular Biology 2024Quote: ... and tissue were analyzed for IL-1ꞵ (human DY200, mouse DY401), IL-6 (mouse DY406), IL-18 (mouse DY7625), and TNF-ɑ (human DY210, mouse DY410) by ELISA (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After transfection neurons were washed twice with NBP and treated with 20 μM (2R)-amino-5-phosphonovaleric acid (AP5; R&D Systems, 0105/10), an NMDA receptor antagonist to prevent transfection-induced neuronal overexcitation ...
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bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... offspring at postnatal day 3 to 6 received 7 intraperitoneal injections with either PBS (controls) or 75 ng of carrier free recombinant mouse interleukin-6 (rmIL6, R&D Systems, Minneapolis MN).
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bioRxiv - Cell Biology 2020Quote: ... IL-6 and TNFα cytokine levels in the media were then measured with DuoSet ELISA kits (R&D systems, catalog number DY406 and DY410) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min at room temperature and then probed with the following primary and secondary antibodies: anti-Mbd4 (Santa Cruz Biotech D-6 or R&D Systems AF5935), anti-Msh6 (AbCam EPR3945) ...
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bioRxiv - Immunology 2024Quote: ... BAL was collected and pooled from groups of 5 mice and cultured for 6 hours at 370 with 5% CO2 in the presence of mouse recombinant IL-4 (R&D systems, 404-ML) and IL-10 (R&D Systems 417-ML ...