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Citations for R&D Systems :
301 - 350 of 478 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The IL-6 and leptin enzyme-linked immunosorbent assay (ELISA) was purchased from R&D systems (Minneapolis, MN), while the Griess assay for nitric oxide (NO ...
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bioRxiv - Bioengineering 2022Quote: ... IL-6 levels from RAW264.7 conditioned medium was quantified using a DuoSet ELISA kit (R&D systems, DY406) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... were seeded on 6 well plate and cultured with recombinant mouse IFNγ (100 units/mL; R&D Systems) and recombinant human IL-1β (5 units/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Physiology 2021Quote: ... and mouse recombinant proteins of IL-6 and TNFα were brought from R&D system (Minneapolis, MN, USA). Mouse anti-eMyHC was purchased form Developmental Studies Hybridoma (DSHB ...
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bioRxiv - Immunology 2021Quote: IFNβ and IL-6 concentrations in organ homogenates were analyzed using mouse DuoSet ELISA kits (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... while Th17 cell cultures were supplemented with IL-6 (20 ng/mL; R&D Systems 206-IL-010), TGF-β (10 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants carefully collected and IL-1β and IL-6 levels measured by ELISA (R&D systems) according to the manufacturer’s specifications.
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bioRxiv - Immunology 2020Quote: ... and cultured for 6 days with recombinant GM-CSF (R&D Systems cat. 215-GM, 50 ng/ml) and IL-4 (R&D Systems cat ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems); PE/Cyanine 7 anti-mouse CD325 (Ep-CAM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ELISA kits for IL-6 (M6000B) and TNF-α (MTA00B) were purchased from R&D Systems (Minneapolis, MN). BSA standard was purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Immunology 2024Quote: ... IL-22 and IL-6 DuoSet enzyme-linked immunosorbent assays were obtained from R&D Systems (Minneapolis, MN) and performed according to the manufacturer’s guidelines.
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bioRxiv - Bioengineering 2024Quote: ... The concentrations of IL-6 in the supernatants were determined using ELISA kits (D6050, R&D Systems, USA), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... IFNβ and IFNλ protein levels from HCT-8 cultures were measured by Human IFN-beta DuoSet ELISA (DY814, R&D Systems) and Human IL29/IL28B (IFN-lambda 1/3 ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Human IL-8/CXCL8 Quantikine ELISA kit and non-immune human IgG control were purchased from R&D Systems (Minneapolis, MN). Phorbol myristate acetate (PMA) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: Mice aged 8-10 weeks were given 0.75 μg/g body weight αCCL11 and αCCL24 (R&D Systems; MAB420 and MAB528) or an equivalent amount of rat IgG (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Physiology 2024Quote: ... Slices were then stained individually in 8- well Ibidi dishes with anti-ENTPD3 (R&D Systems Human CD39L3/ENTPD3 Antibody AF4400) to identify beta cells ...
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bioRxiv - Immunology 2024Quote: ... U-bottom 96 well plates in Essential 8 medium supplemented with 50 ng/mL BMP-4 (R&D Systems, 314-BP010) and 10 μM Rock inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... Additional reagents that were used are 2N Sulfuric Acid Stop Solution (Catalog no. DY994, R&D Systems, Minneapolis, MN), Triphenylphosphine (TPP ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Cell Biology 2020Quote: ... and then were seeded into upper chamber of transwell inserts (8 μM pore size, Costar) with or without coated Matrigel (R&D System). The lower chamber of the transwell was filled with 750 μl medium supplemented with 10% FBS which function as a chemoattractant ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Neuroscience 2024Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed EVs were concentrated with 3 kDa MWCO centrifugal filters and characterized via Luminex Multiplex Assay for TNF-α and IL-8 according to the manufacturer’s instructions (R&D Systems). Results were normalized by total protein content ...
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Microbiology 2024Quote: ... 1β and IL-8 were measured in tissue homogenates from proximal small intestine and colon lesions by commercial porcine ELISA kits (R&D Systems, 158 Abingdon ...
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bioRxiv - Microbiology 2024Quote: ... The levels of the inflammatory cytokines interleukin (IL) 1β and IL-8 were measured by enzyme-linked immunosorbent assays (ELISA) (Porcine-specific DuoSet kits, R&D Systems). The activity of brush border enzymes Aminopeptidase A (ApA) ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The specificity of staining was confirmed by staining with rat IgG (ref:6-001-F, R&D Systems, Biotechne) or rabbit IgG (ab27478 ...
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bioRxiv - Developmental Biology 2021Quote: ... into chondroprogenitor cells (6 days; 20 ng/ml BMP4 [cat. num. 314-BP-010CF; R&D Systems, Minneapolis, MN]). Mesodermal differentiation medium had a base of Iscove’s Modified Dulbecco’s Medium ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were collected and assayed for IL-6 and TNF-α by ELISA Kits (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2020Quote: ... supernatants were collected and IL-6 and TNF-α levels were determined using DuoSet ELISA kits (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Mouse IL-6 was measured in cell supernatants using a DuoSet ELISA kit per manufacturer instructions (R&D Systems).