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Citations for R&D Systems :
401 - 450 of 1355 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Filters were blocked in 5% nonfat milk in TBS-Tween 20 (0.1%) and incubated overnight with rabbit anti-NrCAM (R&D Systems AF8538, 1:1000), mouse anti-Rac1 (BD Biosciences # 610651 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Genetics 2023Quote: ... with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM, 10 µM or 30 µM, R&D Systems, 6977/10) or in the absence of ascorbic acid (normally added to the reprogramming medium at 25 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Physiology 2022Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C in 5% CO2 in a humidified incubator in IMDM with phenol red supplemented with 10% fetal bovine serum (FBS) (R&D systems, Minneapolis, MN), 1mM Sodium Pyruvate ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... This medium is based on DMEM/F12 supplemented with 5% R-Spondin1-conditioned medium (made from Cultrex 293T R-spondin1-expressing cells; R&D Systems, MN, USA), 10% Noggin-conditioned medium (made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2024Quote: ... BAL was collected and pooled from groups of 5 mice and cultured for 6 hours at 370 with 5% CO2 in the presence of mouse recombinant IL-4 (R&D systems, 404-ML) and IL-10 (R&D Systems 417-ML ...
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bioRxiv - Immunology 2024Quote: ... and the adherent irradiated 40LB cells were co-cultured with 5 × 104 naïve splenic B cells in 6 mL of B cell media containing IL-4 (R&D Systems, 1 ng/mL) for 96 hours ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of stop solution (Sulfuric acid) (R&D systems) was added ...
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bioRxiv - Neuroscience 2022Quote: ... Pre warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and the absorbance was monitored at 450 nm with a Fluorostar galaxy plate reader.
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bioRxiv - Neuroscience 2022Quote: ... Pre-warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and absorbance at 450 nm was measured in a Fluorostar galaxy plate reader.
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bioRxiv - Bioengineering 2024Quote: ... hyaluronic acid (HA) (cat. #GLR001, R&D systems, Minneapolis, MN), ethanol (cat ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... caspase-3 (R&D Systems, AF835), Endomucin (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 µM CHIR (R&D Systems), 10 mM A83-01 (Tocris ...
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bioRxiv - Developmental Biology 2024Quote: ... SSEA-3 (R&D Systems, MAB1434) and Alexa Fluor 594 conjugated SSEA-4 (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytokines released from HCEC-1CT cells into cell culture supernatants after 5 h of incubation were measured using a Magnetic Luminex® assay (R&D SYSTEMS, Minneapolis, USA). Cell culture supernatant samples were collected after the incubation for cytokine gene transcription (details for incubation see 2.4. ...
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
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bioRxiv - Genetics 2023Quote: Reprogramming was induced in the presence or absence of IFNγ at 10 ng/ml (from day 0 to 5) (R&D Systems, 485-MI-100). SSEA1+ day 5 iPSCs (4 replicates from different reprogramming rounds ...
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Bioengineering 2023Quote: ... Human mesenchymal stem cells (Passage 5-7) (commercially obtained from Rooster Bio (Frederick, MD)) were cultured in hMSC growth media (R&D Systems, Minneapolis, MN, USA). Adipose-derived stem cells (Passage 3-5 ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Microbiology 2024Quote: ... cecal samples were diluted 1:5 in reagent diluent and quantified by ELISA using the DuoSet Mouse S100A8/S100A9 Heterodimer kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Pathology 2020Quote: ... goat polyclonal anti YM1/Chitinase-3 like 3 (R&D systems, Cat. No. AF2446, 1:400 dilution). Antigen retrieval of HIER2 (EDTA ...
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bioRxiv - Immunology 2024Quote: ... RBCs were washed twice with ice cold RBC staining buffer (PBS supplemented with 0.2% BSA) before staining on ice with anti-CD59AF405 monoclonal antibody (FAB1987V, R&D Systems, 5 ng/µL final concentration) and anti-C3c-FITC polyclonal antibody (ab4212 ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The gel was prepared by mixing 5 mg/mL Cultrex Rat Collagen I matrix gel (3447-020-01, R&D systems, Bio-Techne, Minneapolis, MN, USA), 1 M HEPES (15630-056 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ng/mL mFGF2 (R&D Systems), 1xITS (Millipore Sigma)) ...
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bioRxiv - Bioengineering 2020Quote: ... cleaved caspase-3 (R&D System, MAB835) (1:150 in goat serum ...
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bioRxiv - Genomics 2022Quote: ... 3 µM CHIR99021 (R&D Systems 4423), and 1 µM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Immunology 2021Quote: ... or recombinant ficolin-3 (R&D systems) or human purified C1q (Comp Tech ...
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bioRxiv - Immunology 2020Quote: ... IFNλ2/3 ELISA (R&D Systems DY1789B) and mouse IFNβ ...
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bioRxiv - Cell Biology 2021Quote: ... syndecan-3 (R&D Systems Cat#: FAB3539A) and syndecan-4 (R&D Systems Cat# ...
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bioRxiv - Genomics 2023Quote: ... 3 μM CHIR99021 (R&D Systems 4423) was added to the media ...
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bioRxiv - Genetics 2023Quote: ... 10ng/ml IL-3 (R&D systems) and Ls513 cells were cultured in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... anti-cleaved caspase-3 (R&D Systems), anti-RIPK3 (Abcam,) ...
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bioRxiv - Genomics 2023Quote: ... 3 μM CHIR99021 (R&D Systems 4423), and 1 μM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μM CHIR99021 (R&D Systems 4423), and 1 μM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Cell Biology 2024Quote: ... 3 IU/ml erythropoietin (R&D Systems) and 1% penicillin/streptomycin ...