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Citations for R&D Systems :
251 - 300 of 1355 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Replated cultures of BMDMs were primed with murine interferon-γ (5 ng/mL, R&D Systems) over night and again two hours before stimulation with MDP (10 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... the media were supplemented with 5 ng/mL TGF-β1 (R&D Systems 7754-BH/CF) for 7 days.
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 µl/0.5 µM Ubiquitin-AMC (R&D Systems #U-550-050) substrate ...
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bioRxiv - Neuroscience 2022Quote: ... cell lysates (100 μg of protein) were incubated with O-GlcNAcase (5 μg, R&D Systems) at 37°C for 2 h.
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bioRxiv - Neuroscience 2022Quote: ... 5 μg/ml of TGF-β RII Fc chimera (TGF-β RII Fc) (R&D Systems), was applied to SN cultures 1 h prior to fixation to inhibit TGF-β signaling ...
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bioRxiv - Bioengineering 2023Quote: ... B cell activating medium was supplemented with 5 ug/mL anti-CD40 antibody (R&D systems) and 20 ng/mL IL-4 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... Quantitative analysis for IL-5 was performed using enzyme-linked immunosorbent assay (ELISA) (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 1×105 spirochetes were pretreated with 0–5 μM hSLPI (R&D Systems, #1274-PI- 100) at 33°C for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... media change occurred using Neurobasal medium with 5% FBS (fetal bovine serum, R&D Systems S11550) containing growth factors 10 ng/ml of BDNF (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
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bioRxiv - Cell Biology 2021Quote: ... following 5 min of stimulation with 10 ng/mL PDGF-AA or PDGF-BB (R&D Systems) for the PDGFRα homodimer and PDGFRβ homodimer cell lines ...
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bioRxiv - Genetics 2020Quote: ... sections were incubated with 5 μg/ml goat anti-mouse CD31 (R&D Systems, Inc, Minneapolis, MN) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stimulated overnight with 5 pg/ml of BMP9 in serum-free medium (R&D Systems) and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... following 5 min of stimulation with 10 ng/ml PDGF-BB (R&D Systems, Minneapolis, MN, USA) in the case of the PDGFRαV1/βV2 heterodimer cell line ...
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bioRxiv - Cell Biology 2023Quote: ... the pro- and undifferentiated spermatogonia marker PLZF (day 5-35; 0.6 µg/ml, R&D Systems AF2944), and dual stained with the transcription factor marker FOXO1 (day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with either DMSO or 5 µM Idasanutlin (R&D Systems, #12-35-22-07) for an additional 24 h before cells were harvested for protein and RNA extraction.
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bioRxiv - Developmental Biology 2023Quote: ... transfected cells were provided with 5 ng recombinant mouse TGFβ1 protein (R&D Systems, 7666-MB-005). mCherry fluorescence was determined first by exciting at 570 nm and measuring emission at 610 nm with a 100 msec time delay using a Perkin-Elmer EnVision Xcite Multilabel Plate Reader ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were treated with 100 ng/mL mouse IFNγ or 5 U/mL IFNα (R&D Systems) for 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... heating at 95°C for 5 min in antigen retrieval solution (CTS045, R&D Systems, Minneapolis, MN). After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT ...
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bioRxiv - Immunology 2024Quote: ... dams were treated with 5 μg recombinant murine IL-10 (R&D Systems 417-ML-025/CF) resuspended in sterile PBS ...
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bioRxiv - Immunology 2024Quote: Plates were centrifuged at 825xg for 5 min and supernatants collected for IFNγ ELISA (R&D Systems Mouse IFNγ DuoSet ELISA Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Using a final concentration of 5 ng/mL TGF-β (cat. no. 7666-MB; R&D Systems), cells were incubated for 36h before harvesting ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were cotreated with 20 nM (2S,3S)-2-Amino-3-methyl-N-[2-(4-morpholinyl)ethyl]pentanamide dihydrochloride (LM11a-31)(R&D Systems, Minneapolis, MN, USA, Cat no: 5046) or vehicle solution consisting of DMSO during application of 6-OHDA ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were cultured in R10 media containing 5 ng/mL IL-7 (R&D systems). C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Microvessels with and without 24 h treatment with 5 ng mL-1 human recombinant TNFα (R&D Systems), were perfused with THP1s under low shear stress (∼0.2 dyne cm-2 ...
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bioRxiv - Bioengineering 2021Quote: Cells were treated with brefeldin A (10 μg ml-1, R&D Systems, 1231/5 in differentiation media) for 2 h prior to fixation by immersion in paraformaldehyde (4% [v/v] in 1 × PBS ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... ENAS + 5 ng/ml TGF-β1 (Recombinant Human TGF-β1 Protein, R&D Systems, Cat. No. 240-B). Basal medium + solvent ...
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bioRxiv - Biochemistry 2024Quote: ... 1x mIL-21 (14.9 µg per mouse, n=5 mice; R&D Systems Cat# 594-ML-100/CF), 10x mIL-21 (149 µg per mouse ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 µg mL-1 biotin Polyclonal Goat anti-Fibroblast Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-FGF-2 functionalized beads ...
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bioRxiv - Immunology 2024Quote: ... The mediium was then changed to new culture medium containing 10 ng/ml IL-5 (R&D Systems). On days 8 and 11 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg mL-1 biotin Polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution (R&D Systems, USA), for the generation of anti-VEGF functionalized beads ...
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bioRxiv - Genetics 2021Quote: ... 3 ng/ml mouse IL-3 (R&D Systems) and 20% L-cell conditioned medium ...
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (R&D Systems, Minneapolis, USA), and separately in combination with IL-4 and IL-21 (100 IU each) ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Immunology 2024Quote: ... and incubated with 5 ng/mL IL-4 (cat. no. 404-ML-010, R&D Systems, Minneapolis, MN, USA) (or vehicle control ...
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bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3, 20ng/ml, R&D systems) were used for neural differentiation between days 27 and 41 ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The chamber was prepared by adding 26μl pre-warmed (37°C) CXCL-5 (0, 0.5, 50, 500ng/ml) or CCL2 (0, 0.5, 50, 500ng/ml) (R&D systems) in DMEM to the lower chamber ...
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bioRxiv - Microbiology 2021Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minnesota, MN). For total and differential cell counts ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Immunology 2020Quote: ... two group of 5 mice each were treated with monoclonal anti-mouse IL-25 (mα-IL-25) (R&D Systems) or monoclonal anti-mouse IL-4Rα (mα-IL-4Rα ...