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Citations for R&D Systems :
351 - 400 of 3119 citations for IL 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... 50 ng/ml recombinant human Wnt3a (R&D Systems; day 1 and 2), 1 mM L-Glutamine (L-Glu ...
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bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
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bioRxiv - Biophysics 2024Quote: ... Anti-HIS antibody (R&D systems, BAM050) coated polystyrene beads were prepared as previously described.39 The beads were decorated with native purified Dsn1-6HIS-3Flag kinetochores ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 ng/mL IL-12 (R&D Systems, cat#219-IL-005), 10 ng/mL IL-15 (NCI preclinical repository) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401, R&D Systems) or Mouse TNF-alpha DuoSet ELISA (DY410 ...
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bioRxiv - Immunology 2021Quote: ... IL-15 and/or IL-21 (50 ng/ml; R&D Systems). Human B cells were cultured in FBS-RPMI at 37°C in 48-well plates for 24 ...
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bioRxiv - Immunology 2022Quote: ... IL-6 (25ng/mL) and IL-23 (20ng/mL, R&D Systems). For Th1 differentiation cultures ...
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bioRxiv - Immunology 2019Quote: ... or 1 μM IL-8 (R&D Systems # 208-IL-10/CF) was loaded onto the devices ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant IL-35 cytokine (Cat# 8608-IL, R&D Systems, Minnesota, USA) was obtained and used for cultured CFs treatment ...
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bioRxiv - Immunology 2020Quote: ... and IL-4 (R&D Systems cat. 204-IL, 35 ng/ml) in RPMI 1640 medium supplemented with 10% FBS and 2-Mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... IL-18 and IL-10 according to manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... and IL-1 beta/IL-1 F2 DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... IL-23 (20 ng/ml, 1290-IL-010/CF; R&D Systems), IL-1β (20 ng/ml 201-LB-005/CF ...
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bioRxiv - Immunology 2024Quote: ... IL-5 and IL-13 were measured by ELISA (R&D systems). Results were normalized to cell number using CyQuant cell proliferation assay (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IL-10 clone 948505 (MAB9184) and the isotype controls mouse IgG2a (MAB003) and mouse IgG1 (MAB002) (all from R&D systems).
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-2 (902-MP-010) was purchased from R&D systems (USA). Recombinant human MMP-3 (SRP7783 ...
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bioRxiv - Pathology 2021Quote: ... Samples were assayed into a Human ACE-2 DuoSet ELISA assay (R&D systems), loading equal amounts of protein in duplicate for all samples.
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bioRxiv - Bioengineering 2022Quote: ... or 150 ng/ml recombinant human LOX homolog like 2 (LOXL2, R&D Systems), with media changed every 2-3 days ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-human angiotensin converting enzyme 2 (ACE2; R&D Systems; 1:500). Appropriate species-specific secondary antibodies conjugated to AlexaFluor555 and/or AlexaFluor647 were applied for fluorescent detection ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6 and IL-10 in media (DuoSet, R&D Systems Minneapolis, USA). ELISA procedures were carried out according to manufacturer protocols.
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bioRxiv - Biophysics 2022Quote: ... and rmIL-33 (402-ML, 1399-IL, and 3626-IL, R&D Systems). Stimuli for human ILC2s were recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2020Quote: ... IL-13 (rIL-13; Prepotech) or IL-25 (rIL-25; R&D Systems) (concentration ...
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bioRxiv - Immunology 2022Quote: ... IL-33 concentrations were measured by ELISA (IL-33 duoset, R&D systems) and detected using horseradish peroxidase-conjugated streptavidin and TMB substrate (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and IL-22 (DuoSet ELISA R&D Systems, Minneapolis, MN, USA) IFN-γ (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: IL-1β and IL-6 were detected in tissue lysate using the Mouse IL-1β and IL-6 DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IL-27(p28) in BALF was measured with a mouse IL-27 p28/IL-30 DuoSet ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... supernatants were collected and the released IL-1β were measured by mouse IL-1β/IL-1F2 DuoSet ELISA (Cat. number: DY401) from R&D Systems.
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bioRxiv - Microbiology 2023Quote: The IL-1β levels in supernatant samples were measured using the Mouse IL-1β/IL-1F2 ELISA kit (R&D Systems, DY401), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... ELISAs were performed to quantify IL-1β in these culture supernatants using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: Recombinant human transforming growth factor-β (TGF-β) and fibroblast growth factor-2 FGF-2 were purchased from R&D Systems. BTZ ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma IL-6 was assessed in duplicate using high-sensitivity enzyme-linked immunosorbent assay (ELISA; Quantikine HS Human IL-6 ELISA, R&D Systems, UK) in accordance with the manufacturer’s instructions ...