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Citations for R&D Systems :
201 - 250 of 3119 citations for IL 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Human IL-8/CXCL8 Quantikine ELISA kit and non-immune human IgG control were purchased from R&D Systems (Minneapolis, MN). Phorbol myristate acetate (PMA) ...
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...
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bioRxiv - Microbiology 2023Quote: Harvested supernatants from infected human macrophages were assayed for cytokine levels using ELISA kits for human IL-1α (R&D Systems), IL-18 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 DuoSet ELISA DuoSet and Ancillary Reagent Kit 2 were from R&D Systems.
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bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
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bioRxiv - Biochemistry 2021Quote: Interleukin (IL)-6 in the patient plasma samples was measured by the Quantikine ELISA kit for human IL-6 (R&D Systems, Minneapolis, MN), as previously [29] ...
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: The effective concentration of IL-15 in the samples was assessed using the Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA). All subsequent steps after sample collections were carried out following the instructions provided by the kit ...
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bioRxiv - Immunology 2022Quote: ... Human CSF-1 and IL-34 human serum levels were quantified using ELISA kits (both R&D systems, Mountain View, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Microbiology 2023Quote: ... The culture supernatants were analyzed for cytokines using the Human TNF-α and Human IL-12 DuoSet ELISA kits (R&D Systems) following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... the ELISA kit for human and mouse IL-1β was obtained from R&D Systems. Both were used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Interleukin-6 was measured by Human IL-6 DuoSet ELISA (DY206, R&D Systems, USA). Reagents for interleukin-10 analysis were ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine concentrations were determined using a human IL-8 DuoSet ELISA kit (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... anti-human IL-1β (0.1 μg/ml, goat polyclonal, R&D Systems, AF-201-NA), anti-human IL-37 (2 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems; Bio-techne, Inc.) until use.
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bioRxiv - Microbiology 2019Quote: ... human Flt3L and mouse IL-12p70 Quantikine ELISA kits (R&D systems, Minneapolis, MN, USA) were used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or 10 ng/mL of recombinant human IL-1β (R&D Systems; 201-LB-005) for the indicated times ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated overnight with anti-human IL-1β (1:100, 615417, R&D Systems) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 25 ng/ml human IL-17 recombinant protein (R&D Systems). Vehicle control or 25 ng/ml human TNF-α recombinant protein were used as controls ...
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bioRxiv - Cell Biology 2023Quote: ELISA was performed using the human IL-6 Duo-Set (R&D Systems, cat.# DY206), human GDF-15 Duo Set (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... IL-12 and recombinant human TGF-β1 were purchased from R&D Systems (Abingdon, UK).
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bioRxiv - Immunology 2021Quote: ... and Rat IL-2 ELISpot Kit (R&D Systems, Cat. No.: XEL502). ELISpot plates precoated with capture antibody were blocked with LCM for at least 30 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 ng/mL recombinant mouse IL-2(R&D system, MN, USA), and 50 μM β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 ng/mL recombinant mouse IL-2 (R&D system, MN, USA), and 50 μM β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and 5 ng/ml IL-2 (carrier-free; Tocris R&D Systems) added with cells ...
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bioRxiv - Immunology 2022Quote: ... in the presence of IL-2 (100 U/ml, R&D Systems) and IL-7 (50 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 24h with 100ng/ml media of human recombinant GBA (rhGBA-His (R&D Systems)).
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2019Quote: ... ELISAs were performed according to the manufacturer’s instructions for IL-10 (Quantikine®HS ELISA Human IL-10 Immunoassay, R&D Systems, Inc., Cat# HS100B), sCD14 (Quantikine® ELISA Human sCD14 Immunoassay ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Physiology 2021Quote: ... IL-1β plasma level was evaluated using Human IL1-β ELISA Kit (DLB50, R&D Systems) according to the manufacturer procedure.
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bioRxiv - Physiology 2024Quote: ... then stimulated with recombinant human IL-4 at 50 ng/mL (R&D Systems, Minneapolis, MN) in BEBM medium for 6 h or BEBM medium as control condition ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with recombinant human IL1β (208-IL-010, R&D Systems, Minneapolis, MN, USA) for 30 min before immunofluorescence ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant human IL6 (cat# 206-IL-050) was purchased from R&D Systems (Minneapolis, MN, USA) and stored at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... for 2 days in R10 medium supplemented with IL-2 (10ng/ml, 402-ML-020, R&D Systems) for 2 days ...
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Developmental Biology 2019Quote: ... goat anti-human VEGFR-2 (R&D systems, AF357), rabbit anti-human Rab5 (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... or human ACE-2 Fc (chimera, R&D Systems) in serial dilutions of 500-31.3 ng/ml PBS-T were incubated (n = 6 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 2 ng/ml human BMP4 (R&D Systems). The plates were centrifugated at 210 g for 4 min to promote cell aggregation before incubation at 37 °C under 5% CO2 normoxic condition ...
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bioRxiv - Immunology 2022Quote: ... in the presence of IL-2 (10 ng ml−1, R&D Systems), anti-IL-10 (50 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 20 IU/mL rhIL-2 (R&D Systems Cat# 202-IL) for 72±4 hours at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... and cultured in media containing IL-2 (5 ng/ml; R&D Systems), in the presence or in the absence of GGSK2691805A (5 μM) ...
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bioRxiv - Immunology 2020Quote: ... and an Equine IL-2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). Measurements were performed in duplicate according to the manufacturer’s protocol.