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Citations for R&D Systems :
351 - 400 of 690 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: Circulating AAV-expressed human ANGPTL3 protein levels were measured from plasma using commercially-available ELISA kits (plasma diluted 1:4 and used with R&D Systems #DANL30). The assay is specific for detection of human ANGPTL3 protein ...
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 min on a rotator at 4 °C and then transferred to a new tube with 5 μl TSC1 antibody (R&D Systems, MAB4379) or 25 μl mouse IgG Control (SC-2025 ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Immunology 2024Quote: ... BAL was collected and pooled from groups of 5 mice and cultured for 6 hours at 370 with 5% CO2 in the presence of mouse recombinant IL-4 (R&D systems, 404-ML) and IL-10 (R&D Systems 417-ML ...
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bioRxiv - Immunology 2024Quote: ... and the adherent irradiated 40LB cells were co-cultured with 5 × 104 naïve splenic B cells in 6 mL of B cell media containing IL-4 (R&D Systems, 1 ng/mL) for 96 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse interleukin-3 (IL-3) (R&D Systems, 403-ML) and 10 ng/mL mouse interleukin-6 (IL-6 ...
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bioRxiv - Molecular Biology 2020Quote: Randomly selected sections coming from ovarian tissue of least 3 different individuals were used for immunohistochemistry for Anti-active Caspase-3 (AC-3, 1:1000, AF-835, R&D Systems), γH2AX (1:250 IHC-00059 ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Fluorogenic Peptide Substrate VII: Z-Leu-Arg-4-methyl-7-coumarylamide (Z-LR-AMC)(Catalog # ES008) were purchased from R&D Systems (Minneapolis, MN, USA). The enzyme reactions were initiated upon addition of ice-cold of hrCTSL (96pM ...
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma concentrations of fatty acid binding protein 4 (FABP4) and leptin were determined using the xMAP technology Luminex assays (R&D Systems, Minneapolis, MN, USA) and the Luminex MAGPIX System (Luminex Corp. ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Immunology 2024Quote: ... 1 × 105 B cells were added to each well in 6 mL of B cell media containing either IL-4 (R&D Systems, 1 ng/mL) or IL-21 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... α3 (MAB1345; R&D systems), β4 (NBP1-43369) ...
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bioRxiv - Cancer Biology 2024Quote: ... Type 3 (R&D Systems); coenzyme Q10 (CoQ) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with horse serum (7% in PBS) for 20 minutes prior to overnight incubation at 4°C with goat anti- intracellular adhesion molecule-1 (ICAM1) (R&D Systems, Inc, Minneapolis, MN, USA), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Physiology 2024Quote: ... 50μL serum was 1:4 diluted with 150 μL dilution buffer to measure glucagon concentration using commercial ELISA kits (Mouse, DGCG0, R&D Systems; human, D711361, Sangon Biotech). All procedures were performed by following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin) with added FGF ligands (Fgf7; 4 µM, R&D Systems, Cat. No. 5028-KG-025), the γ-secretase inhibitor DAPT (10 µM ...
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bioRxiv - Immunology 2023Quote: ... the pellets obtained after centrifugation of the BAL fluid were pooled and stained for 30 min at 4°C with unconjugated polyclonal goat anti-mouse Ym1/2 (AF2446, R&D Systems; 1:100 diluted in PBS). After washing ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the DE monolayer was incubated for 4 days with the hindgut induction medium supplemented with 500 ng/mL of FGF4 (R&D Systems, cat # 235-F4-025/CF) and 3μM of CHIR99021 (STEMCELL Technologies ...