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Citations for R&D Systems :
601 - 650 of 690 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Pathology 2021Quote: ... Gdf8 and Smad2/3 proteins were quantified by ELISA methods (GDF8/Myostatin Quantikine ELISA kit, Cat # DGDF80, R&D Systems, Minneapolis ...
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bioRxiv - Cell Biology 2022Quote: ... NED] and 3U.ml−1 EPO [Roche]) supplemented with 1 ng.ml−1 IL→ 3 and 10 ng.ml−1 SCF (both R&D Systems) and 1 µg.ml−1 doxycycline for 4 days ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... and levels of tumor necrosis factor-α (TNF-α) were assessed by ELISA within 3 days (DY410; R&D Systems). The cytokines TNF-α ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse IL-3 (403-ML) and 10 ng/mL mouse L-6 (406-ML) (R&D Systems). Total RNA was extracted using the RNeasy Mini kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... 300 ng/mL SHH day 0 to 9 and 400 ng/mL IGFBP-3 (R&D systems, #675-B3-025) from day 7 to 14 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysate from supernatant was used for IP with 3 μg of CXCL13/IgG antibody (AF801, R&D system) and 20μl precleared protein A beads (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... injected 9 times spread over 3 days with recombinant mouse IL-34 (74,16 pg, 5195-ML-010; R&D systems) or with sterile D-PBS as control.
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bioRxiv - Developmental Biology 2024Quote: ... The following growth factors were present for the first 3 days of differentiation: 25 ng/ml BMP4 (R&D Systems), 25 ng/mL Activin A (Miltenyi Biotech ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IFNλ from intestinal biopsies were measured by Mouse IL28B/ IFN-lambda 3 DuoSet ELISA (DY1789B, R&D Systems). Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lambda interferon was evaluated by Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Interleukin-6 was measured by Human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems, UK). Plates were left to incubate at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: For sTREM2 measurements, multi array 96-well plates (MSD, L15XA-3) were coated with mouse anti-TREM2 (R&D systems, AF1729) capture antibody O/N at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... PSA was measured in lateral vaginal wall swabs using Human Kallikrein 3/PSA Quantikine ELISA kits (R&D Systems, MN, USA).
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from CH12 cells cultured for 3 days with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... and TNF levels were determined in the supernatants of Calu-3 and macrophage cultures using ELISA kits following the manufacturer’s instructions (R&D Systems). HMGB-1 was determined in the cell-free TA supernatants and in the supernatants of Calu-3 cultures using immunoassay following the manufacturer’s instructions (Novus Biologicals) ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then incubated with a 5 µg/mL of human HNF-3 beta/FoxA2 antibody (R&D Systems; AF2400) for 1.5 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Immunology 2024Quote: ... we administered 3 doses (100 uL) of either vehicle control or 50 ug of CCL24 neutralizing antibody (MAB528, R&D systems) via intraperitoneal injection before TAC and during the first week of recovery ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... LCs were added at 3 x 104 per well along with anti-PIGF (10 μg/ml, R&D systems, #MAB465-500), anti-VEGFR3 (5 μg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were split 1:3 in SF-D media containing recombinant human (rh) VEGF (5 ng ml-1; R&D Systems), rhBMP4 (10 ng ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were cultured to achieve 80-90% confluency, and treated with 3 μM CHIR99021 (CHIR, Stem-RD) and 100 ng/ml Activin A (R&D systems) in basal medium RPMI1640 (Cellgro ...
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bioRxiv - Systems Biology 2021Quote: ... HGF 50 ng/ml; NT-3 10 ng/ml; PDGF-BB 10 ng/ml; GDNF 10 ng/ml; All from R&D systems). Ligand treatment was started at the time of tetracycline induction ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Pathology 2020Quote: ... was measured immediately before injection and at 3 days after injection using a Mouse Lipocalin-2/NGAL Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems) to ensure successful injection of folic acid ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...