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• KIR3DL1 and Tox identify clonally expanded encephalitogenic neuron-specific CD8+ T cells in autoimmune encephalitis

  Sylvain Perriot, et al., bioRxiv - Immunology 2024

  Quote: ... and TNFα (1ng/ml, R&D Systems) for 48 hours to induce HLA class I enhancement (see Human-iPSC-derived neuron preparation section and Supplementary Table 5) ...
• Relative rDNA copy number is not associated with resistance training-induced skeletal muscle hypertrophy and does not affect myotube anabolism in vitro

  Joshua S. Godwin, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 55 ng/ml dexamethasone (R&D Systems, distributed by VWR ...
• CXCR2-mediated recruitment of adaptive NK cells with NKG2C/HLA-E dependent antigen-specific memory enhances tumor killing in ovarian cancer

  Yizhe Sun, et al., bioRxiv - Immunology 2024

  Quote: ... or a control isotype-matched antibody IgG (clone Poly4053) (BioLegend, R&D systems) were added at the primary phase (day 0 and 7 ...
• Effective Gene Therapy for Metachromatic Leukodystrophy Achieved with Minimal Lentiviral Genomic Integrations

  Lucas Tricoli, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Gels were probed with antibodies against hARSA (R&D systems, Minneapolis, MN), CD63 (Abcam ...
• Impaired drainage through capillary-venous networks contributes to age-related white matter loss

  Stefan Stamenkovic, et al., bioRxiv - Neuroscience 2024

  Quote: ... and Mouse Aminopeptidase N/CD13 (Goat anti-CD13, R&D Systems; AF2335, dilution: 1:100). Primary antibodies were incubated for 10 days at 37°C ...
• Impaired drainage through capillary-venous networks contributes to age-related white matter loss

  Stefan Stamenkovic, et al., bioRxiv - Neuroscience 2024

  Quote: ... Pericytes were labeled by combining PDGFRβ (Goat anti-PDGFRβ, R&D Systems; AF1042, dilution: 1:100) and Mouse Aminopeptidase N/CD13 (Goat anti-CD13 ...
• Amelioration of signalling deficits underlying metabolic shortfall in TREM2^R47H human iPSC-derived microglia

  Foteini Vasilopoulou, et al., bioRxiv - Neuroscience 2024

  Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
• Amelioration of signalling deficits underlying metabolic shortfall in TREM2^R47H human iPSC-derived microglia

  Foteini Vasilopoulou, et al., bioRxiv - Neuroscience 2024

  Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
• SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and pain

  Sonali S. Mali, et al., bioRxiv - Neuroscience 2024

  Quote: ... Ubiquitin-Rh110 (R&D Systems, #U-555) at 670 nM in phenol-free RPMI ...
• Translational validation of shotgun proteomics findings in cerebrospinal fluid of sporadic cerebral amyloid angiopathy patients

  Marc Vervuurt, et al., bioRxiv - Neuroscience 2024

  Quote: ELISAs were used to quantify CTSB and CTSS levels in CSF (both R&D DuoSets, R&D Systems, Minneapolis, USA). CSF samples were diluted 20x with reagent diluent prior to ELISA analysis ...
• SARS-COV-2 induces blood-brain barrier and choroid plexus barrier impairments and vascular inflammation in mice

  Haowen Qiao, et al., bioRxiv - Neuroscience 2024

  Quote: ... CD13 (1:200; R&D System: AF2335); IBA1 (1:800 ...
• Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes

  Rahul Atmaramani, et al., bioRxiv - Neuroscience 2024

  Quote: ... Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), and Doxycycline Hydrochloride (1ug/ml ...
• Cell circuits underlying nanomaterial specific respiratory toxicology

  Carola Voss, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... IL33 (all R&D Systems) according to the protocols provided by the manufacturer ...
• Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes

  Rahul Atmaramani, et al., bioRxiv - Neuroscience 2024

  Quote: ... Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), EdU (5-ethynyl-2’-deoxyuridine ...
• Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes

  Rahul Atmaramani, et al., bioRxiv - Neuroscience 2024

  Quote: ... Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml ...
• Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes

  Rahul Atmaramani, et al., bioRxiv - Neuroscience 2024

  Quote: ... Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml ...
• Rat primary cortical cell tri-culture to study effects of amyloid-beta on microglia function

  Hyehyun Kim, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse IL-34 (R&D Systems) TGF-β2 (Peprotech) ...
• Role of histone variants H2BC1 and H2AZ.2 in H2AK119ub nucleosome organization and Polycomb gene silencing

  Xiangyu Shen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 15% murine ESC defined FBS (R&D System, S10250), 1% GlutaMax (Gibco ...
• A human papillomavirus 16 E2-TopBP1 dependent SIRT1-p300 acetylation switch regulates mitotic viral and human protein levels

  Apurva T. Prabhakar, et al., bioRxiv - Molecular Biology 2024

  Quote: ... supplemented with 10% fetal bovine serum (FBS) (R&D Systems) and 1.5 mg/mL G418 sulfate as described previously (21 ...
• Activity-dependent regulation of vascular cholesterol metabolism acts as a negative feedback mechanism for neurovascular coupling

  CP Profaci, et al., bioRxiv - Neuroscience 2024

  Quote: ... Primary antibodies against LDLR (R&D Systems AF2255) and CD31 (BD Biosciences 553370 ...
• Local Delivery of Soluble Fractalkine (CX3CL1) Peptide Restore Ribbon Synapses After Noise-Induced Cochlear Synaptopathy

  Vijayprakash Manickam, et al., bioRxiv - Neuroscience 2024

  Quote: ... cochlear frozen mid-modiolar cross sections or whole mounts were incubated overnight at room temperature with combinations of following primary antibodies: goat anti-CD45 (R&D Systems, catalog # AF114 ...
• Local Delivery of Soluble Fractalkine (CX3CL1) Peptide Restore Ribbon Synapses After Noise-Induced Cochlear Synaptopathy

  Vijayprakash Manickam, et al., bioRxiv - Neuroscience 2024

  Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
• Local Delivery of Soluble Fractalkine (CX3CL1) Peptide Restore Ribbon Synapses After Noise-Induced Cochlear Synaptopathy

  Vijayprakash Manickam, et al., bioRxiv - Neuroscience 2024

  Quote: ... Level of cochlear endogenous FKN (R&D systems, #DY472) or TT injected (exogenous ...
• Local Delivery of Soluble Fractalkine (CX3CL1) Peptide Restore Ribbon Synapses After Noise-Induced Cochlear Synaptopathy

  Vijayprakash Manickam, et al., bioRxiv - Neuroscience 2024

  Quote: ... recombinant control peptide (R&D systems, cat. #4460, Accession # P01863) is mouse melanoma cell line ...
• Local Delivery of Soluble Fractalkine (CX3CL1) Peptide Restore Ribbon Synapses After Noise-Induced Cochlear Synaptopathy

  Vijayprakash Manickam, et al., bioRxiv - Neuroscience 2024

  Quote: ... recombinant full length membrane-bound Fractalkine (R&D systems, cat. #472-FF, Accession # O35188) is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems ...
• A postnatal molecular switch drives the activity-dependent maturation of parvalbumin interneurons

  Monika Moissidis, et al., bioRxiv - Neuroscience 2024

  Quote: ... and the replicate of mouse anti-ERRγ antibody (R&D Systems #PP-H6812-00), using SEACR (Sparse Enrichment Analysis for CUT&RUN ...
• Interleukin-6 induces nascent protein synthesis in human DRG nociceptors via MNK-eIF4E signaling

  Molly E. Mitchell, et al., bioRxiv - Neuroscience 2024

  Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
• Benchmark of cellular deconvolution methods using a multi-assay reference dataset from postmortem human prefrontal cortex

  Louise A. Huuki-Myers, et al., bioRxiv - Neuroscience 2024

  Quote: ... and OLIG2 (R&D systems, Cat No ...
• Embryonic motor neuron programming factors reactivate immature gene expression and suppress ALS pathologies in postnatal motor neurons

  Emily R. Lowry, et al., bioRxiv - Neuroscience 2024

  Quote: ... or human SOD1 (1:250, R&D Systems MAG 3418). Sections were incubated for 18-24h in primary antibody solution containing 1% bovine serum albumin ...
• L-Type Ca²⁺ channels and TRPC3 channels shape brain pericyte Ca²⁺ signaling and hemodynamics throughout the arteriole to capillary network in vivo

  Jessica Meza-Resillas, et al., bioRxiv - Neuroscience 2024

  Quote: ... goat anti-CD31 (1:200, R&D systems, AF3628), rabbit-anti-Cav1.2 (1:200 ...
• Low intensity repetitive transcranial magnetic stimulation enhances remyelination by newborn and surviving oligodendrocytes in the cuprizone model of toxic demyelination

  Phuong Tram Nguyen, et al., bioRxiv - Neuroscience 2024

  Quote: ... Primary antibodies included goat anti- PDGFRα (1:100, R&D Systems AF1062), rabbit anti-OLIG2 (1:400 ...
• A postnatal molecular switch drives the activity-dependent maturation of parvalbumin interneurons

  Monika Moissidis, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-ERRγ (1:100, R&D Systems #PP-H6812-00), rabbit anti-MEF2c (1:100 ...
• A postnatal molecular switch drives the activity-dependent maturation of parvalbumin interneurons

  Monika Moissidis, et al., bioRxiv - Neuroscience 2024

  Quote: ... or 2 μg of mouse anti-ERRγ antibody (R&D Systems #PP-H6812-00, one replicate) in CUT&RUN Wash buffer for 10min with rotation at 4°C ...
• Social transmission of inflammation in mice

  Silvia Castany Quintana, et al., bioRxiv - Neuroscience 2024

  Quote: ... or goat anti-Lipocalin-2 (MAB1757; 1:1000; R&D systems) overnight at room temperature ...
• NRP1-expressing monocyte/macrophages promote arteriogenesis in the ischemic limb

  JS Cho, et al., bioRxiv - Pathology 2024

  Quote: ... Cells were then incubated for 1 h at 37°C with mouse anti-human PECAM1 (R&D Systems), which was diluted 1:1000 in 1% BSA/PBS using 300 μl/well ...
• An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal and neural cells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... FGF4 (500 ng/ml, R&D Systems, #235-F4/CF), CHIR99021 (3 μM ...
• An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal and neural cells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... Noggin (100 ng/ml, R&D Systems, #6057-NG/CF), EGF (100 ng/ml ...
• An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal and neural cells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... EGF (100 ng/ml, R&D Systems, #236-EG), CHIR99021 (2 μM ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... the laminin α1 chain (rat monoclonal, MAB4656, R&D Systems, Minneapolis, USA), myogenic differentiation antigen 1 (MYOD1 ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... myogenin (MYOG) (mouse monoclonal, MAB66861, R&D Systems, Minneapolis, USA), myogenic factor 5 (MYF5 ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... myogenic factor 5 (MYF5) (mouse monoclonal, MAB4027, R&D Systems, Minneapolis, USA), myosin heavy chain (MYHC ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... MYOG (mouse monoclonal, MAB66861, R&D Systems, Minneapolis, USA), myosin heavy chain 2 (MYH2 ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... MYHC (mouse monoclonal, MAB4470, R&D Systems, Minneapolis, USA), desmin (mouse monoclonal ...
• A Novel Mouse Model for LAMA2-Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

  Dandan Tan, et al., bioRxiv - Neuroscience 2024

  Quote: ... myosin heavy chain (MYHC) (mouse monoclonal, MAB4470, R&D Systems, Minneapolis, USA), desmin (mouse monoclonal ...
• Transcriptomic signature and pro-osteoclastic secreted factors of abnormal bone marrow stromal cells in fibrous dysplasia

  Zachary Michel, et al., bioRxiv - Pathology 2024

  Quote: ... RANKL was detected using Mouse TRANCE/RANKL/TNFSF11 Quantikine ELISA Kit (R&D systems, MTR00), Ephrin B2 ...
• Human iPSC-derived Committed Cardiac Progenitors Generate Cardiac Tissue Grafts in a Swine Ischemic Cardiomyopathy Model without Triggering Ventricular Arrhythmias

  Amish N. Raval, et al., bioRxiv - Pathology 2024

  Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
• Pharmacological Characterization of SDX-7320/Evexomostat: a Novel Methionine Aminopeptidase Type 2 Inhibitor with Anti-Tumor and Anti-Metastatic Activity

  Peter Cornelius, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: Enzyme activity was measured at room temperature using a coupled assay system (R&D Systems) containing the fluorogenic substrates Met-Gly-Pro-AMC (for MetAP1 ...
• Innate Immune Activation and Mitochondrial ROS Invoke Persistent Cardiac Conduction System Dysfunction after COVID-19

  Deepthi Ashok, et al., bioRxiv - Pathology 2024

  Quote: ... Cytokines released from hiPSC-CMs into the supernatants were analyzed using Proteome Profiler Human XL Cytokine Array Kit (Cat. No. ARY022B R&D Systems). Chemiluminescence from the membrane was detected and scanned on an iBright Imaging System FL1000 (Thermo Fisher Scientific) ...
• Deletion of absent in melanoma (AIM) 2 gene alters bone morphology

  Zhenwei Gong, et al., bioRxiv - Pathology 2024

  Quote: ... and horseradish peroxidase-3,3′-diaminobenzidine system (cat. no. cts005; R&D Systems, Minneapolis, MN, USA). Images were acquired by Aperio CS2 Scanner (Leica Biosystems ...
• A weighted and cumulative point system for accurate scoring of intestinal pathology in a piglet model of necrotizing enterocolitis

  Simone Margaard Offersen, et al., bioRxiv - Pathology 2024

  Quote: ... and 4 by commercial porcine ELISA kits (R&D Systems, Abingdon, Oxfordshire, United Kingdom) according to the manufacturer’s instructions and expressed as picograms per milligram of wet tissue [13] ...