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Citations for R&D Systems :
3901 - 3950 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and incubated with 10 nM and 1 µM human SLPI (R&D Systems, #1274-PI-100) and murine SLPI (produced in lab as described above ...
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bioRxiv - Developmental Biology 2024Quote: ... EPO and M-CSF using commercial Human CXCL12/SDF-1 alpha Quantikine ELISA (R&D Systems), LEGEND MAX™ Human Erythropoietin (EPO ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies for proteins of interest were anti-IL-33 (1:50, cat.no.AF3626, R&D Systems), anti-AdipoR2 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 ng/mL human FGF basic/FGF2/bFGF (R&D Systems #233-FB-025/CF). After passage one ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2mM ascorbic acid and 1 ng/mL recombinant human FGF (R&D system, 233-GMP-025). The adherent cells were split by trypsinization at 80% confluency and frozen at passage 2 (50% αMEM ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used for immunostaining: goat anti-PECAM1 (1:250, AF3628, R&D Systems), rabbit anti-DACH1 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin-streptomycin and 50 ng/mL recombinant human GM-CSF (R&D Systems, 215-MC). M1 macrophage differentiation medium was prepared using M1 macrophage culture medium supplemented with 100 ng/mL of LPS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies used for immunoblotting and immunofluorescence studies included those for Gas1 (1:1000, R&D Systems, Inc ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant human Shh N-terminal fragment (1 ng/ml; R&D Systems, #8908-SH-005) was then added to the same serum-free medium for 12 hours or overnight.
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bioRxiv - Cancer Biology 2024Quote: ... DKK1 levels were measured using Human Dkk-1 Quantikine ELISA Kit (R&D systems, Cat# DKK100B), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: The following primary antibodies were used: sheep anti-ESGP/MymX (1:1000; R&D Systems; AF4580), mouse anti-MymK (Zhang et al ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma concentrations of fatty acid binding protein 4 (FABP4) and leptin were determined using the xMAP technology Luminex assays (R&D Systems, Minneapolis, MN, USA) and the Luminex MAGPIX System (Luminex Corp. ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were administered either a 5 μL volume of phosphate-buffered saline (PBS) containing 10 μg/kg of carrier-free rmIL-1β (R&D Systems, Minneapolis, MN) or PBS alone (control ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Cell Biology 2020Quote: ... were covered with HUVECs and embedded 24 hours later in a 2,5 mg/mL fibrin gel in the presence or absence of VEGFA-165 at 5 ng/mL (R&D systems, Minneapolis MN, USA). Conditioned media from papillary or reticular fibroblasts were added on top of the gel and renewed every day for four days ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Genetics 2023Quote: ... with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM, 10 µM or 30 µM, R&D Systems, 6977/10) or in the absence of ascorbic acid (normally added to the reprogramming medium at 25 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C in 5% CO2 in a humidified incubator in IMDM with phenol red supplemented with 10% fetal bovine serum (FBS) (R&D systems, Minneapolis, MN), 1mM Sodium Pyruvate ...
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bioRxiv - Cell Biology 2024Quote: ... This medium is based on DMEM/F12 supplemented with 5% R-Spondin1-conditioned medium (made from Cultrex 293T R-spondin1-expressing cells; R&D Systems, MN, USA), 10% Noggin-conditioned medium (made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Neuroscience 2020Quote: The R&D Systems rat cytokine XL ELISA Proteome Profiler array (ARY30, R&D Systems Inc. ...
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bioRxiv - Immunology 2020Quote: ... The Mouse SP-D Immunoassay Quantikine ELISA kit from R&D system (Minneapolis, MN, USA) was used according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2023Quote: An R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Cancer Biology 2023Quote: An R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Cancer Biology 2024Quote: An R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse Lyve1 (1:500, #DP35135P, Acris Antibody GmbH, Herford, Germany) and sheep anti-mouse Trem2 (1:50, #AF1729, R&D Systems), and rabbit anti-mouse Ki67 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: Goat polyclonal antibodies against NEGR1 (AF5394) used for immunocytochemistry (IC: 1:100) and immunohistochemistry (IHC: 1:1000) were from R&D systems. Rabbit polyclonal antibodies against NEGR1 (ab101809 ...
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bioRxiv - Bioengineering 2022Quote: ... Positive control neutrophils were incorporated into MCDB-131 Complete Media at a concentration of 900k cells.mL-1 and supplemented with 10 ng.mL-1 IL-8 (rh CXCL8/IL-8, R&D Systems, Minneapolis, MN) for 15 minutes before rinsing and stained ...
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bioRxiv - Neuroscience 2021Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Immunology 2022Quote: ... 1×106 PMBCs were incubated with 20 ng of MIP-1β (recombinant human CCL4L1/MIP-1β isoform LAG-1; R&D Systems) for 30 min at 37°C ...
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bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...
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bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
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bioRxiv - Neuroscience 2022Quote: ... The neurofilament marker SMI32 (mouse monoclonal, 1:1000; Sternberger Monoclonals) and a goat anti-osteopontin antibody (OPN, 1:1000; R&D Systems) were used to label alpha RGCs ...
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bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
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bioRxiv - Microbiology 2024Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1(R&D systems, Cat# E-522B), 2 ...