Labshake search
Citations for R&D Systems :
3651 - 3693 of 3693 citations for 1 4 Bis acetyloxy 3 dodecylsulfanyl 2 naphthyl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed and blocked in PBS with 2% donkey serum and 2% bovine serum albumin for an hour and incubated with rabbit anti-IBA1 (WAKO, cat#019-19741, RRID:AB_839504, 1:500) and goat anti-GPNMB (R&D Systems, cat#AF2550, RRID:AB_416615, 3µg/ml) primary antibodies in PBS with 2% donkey serum overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for in vitro biotinylation (dilutions for each antibody are indicated in parentheses): chicken polyclonal antibody anti-neurofascin (1:1000; R&D Systems Cat# AF3235, RRID:AB_10890736), rabbit polyclonal anti-NrCAM (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cancer Biology 2023Quote: ... and the signalling ligands identified from the ligand-receptor analysis (Figure 4C): murine WNT5A (250 ng mL−1, R&D Systems 645-WN-010/CF), murine SEMA3A (250 ng mL−1 ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Neuroscience 2023Quote: ... A Nanoject II (Warner Instruments) was used to infuse 1 μl of vehicle (DMSO + saline) or AQ-RA 741 (R&D SYSTEMS, in DMSO + saline) before stimulus presentation on experimental days 1 and 2 under isoflurane anesthesia ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used at indicated concentrations for the immunostaining of compresstome sections or iDisco whole lobe samples: goat anti-EPCR (R&D Systems, AF2749, 1:100), rat anti-Endomucin (Santa Cruz Biotech ...
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bioRxiv - Neuroscience 2024Quote: Animals were randomly assigned to experimental groups and injected intrathecally with saline or CCL2 (1 µg/rat, dissolved in 0.1% BSA in sterile saline; R&D Systems #3144-JE-025/CF) on postoperative days (PODs ...
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Microbiology 2024Quote: ... cecal samples were diluted 1:5 in reagent diluent and quantified by ELISA using the DuoSet Mouse S100A8/S100A9 Heterodimer kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1×107 cells and 0.1 μL of each antibody (V5 tag antibody, Invitrogen 451098; His tag antibody, Cell Signaling Technology 14930; or Myc tag antibody, R&D Systems IC3696P) were incubated (for 45 min ...
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bioRxiv - Bioengineering 2024Quote: ... the primary antibody was washed off with PBS and secondary antibody (NorthernLights™ Anti-goat IgG-NL637 Cat: NL002; R&D Systems, 1:50 dilution) was added to tissue sections and incubated for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryoid bodies (EB) were harvested and seeded in serum-free differentiation media supplemented with 1 ng/ml human Activin A (R&D Systems Cat#. 338-AC) and 1 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... cells were starved in 0.1% FBS for 24 hours prior to addition of EGF (R&D systems #236-EG, diluted in 1 mg/ml BSA/PBS) to 25 ng/μl or carrier alone for 5 min at 37ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% NaN3 in D-PBS) containing optimal concentration determined by antibody titration of the Alexa Fluor® 647-conjugated IGDCC3 extracellular antibody (1:20; R&D Systems; Cat. No. FAB8559R). After a 30 min incubation in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinantly expressed TIM-4 in HEK293 cells (Q96H15) and podocalyxin-like protein 1 recombinantly expressed in NS0 cells (AAB61574.1) were purchased from R&D Systems (9407-TM, 1658-PD-050). Recombinantly expressed LAMP-4 in HEK293 cells (P34810 ...
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bioRxiv - Genetics 2024Quote: The concentration of IGF-1 in the liver was quantified using the Mouse/Rat IGF-I/IGF1 Quantikine ELISA Kit (MG100, R&D Systems, Inc., Minneapolis, MN, USA). Liver protein lysates were diluted at a ratio of 1:6 in calibrator diluent and the assay was performed following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Physiology 2024Quote: ... Protein expression was visualised using immunofluorescence staining of mesenteric artery rings and the following antibodies: anti-CD31 (PECAM, # AF3628, R&D Systems, 1:1000 dilution, raised in goat); anti-TRPV4R (# ACC-034 ...
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... with mTsER™ Plus media supplemented with the SMAD inhibitors SB-431542 (Medchem, Cat# HY10431; 10 μm final) and Dorsomorphin (R&D Systems. Cat# 309310; 1 μm final), and the Rho-associated proteinase kinase (ROCK ...
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bioRxiv - Bioengineering 2024Quote: ... and inflammatory factors (TNF-α, IL-1β, IL-6, and MCP-1) in different therapeutic agents were measured by Quantikine® ELISA kit (R&D Systems Inc. Minneapolis, MN, USA) in triplicate ...
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bioRxiv - Cancer Biology 2024Quote: ... the measurement was paused and then 5 µL of a 1 mg/mL solution in PBS was added (human recombinant CD206, Cat # 2534-MR-050/CF from R&D systems, reconstituted with 50 µL of mQ); final concentration of CD206 in the cell ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...