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Citations for R&D Systems :
3501 - 3550 of 3693 citations for 1 4 Bis acetyloxy 3 dodecylsulfanyl 2 naphthyl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
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bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150 ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Molecular Biology 2024Quote: ... ELISAs were performed to quantify IL-1β in these culture supernatants using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) per the manufacturer’s instructions.
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...
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bioRxiv - Immunology 2024Quote: ... Total THP levels of a subset of urine samples (diluted 1:10) was determined by ELISA (catalog no. DY5144-05, R&D Systems) per manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... treatment wells received 10 ng/ml (for migration assays, co-culture assays proteomic screening) and or 1 ng/ml (for RNA-seq) of rhIFNγ (R&D systems).
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used in this study: Goat IgG against VE-cadherin (1:100, AF1002, R&D Systems, Minneapolis, MN), pY658 and pY685 VEC (1:40 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1 % Triton-X-100 in PB (0.1 mol/L, pH 7.4) and incubated with goat anti-CD31 (1:100, R&D systems, Minneapolis, USA). As secondary antibody biotinylated anti-goat IgG (1:500 ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining volume was divided into two tubes and incubated on ice with either 1 μg of anti-galectin-9 (AF2545, R&D systems) or goat isotype control (Jackson Immunoresearch ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then washed with FACS buffer and labeled with 1 µl/well of phycoerythrin-conjugated anti-mouse secondary (F0102B, R&D Systems) 1 hour at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for immunofluorescent staining during this study: mouse anti-β III-tubulin (1:500; R&D Systems, MAB1195), custom rabbit anti-JCV N (1:200 ...
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bioRxiv - Immunology 2024Quote: ... ex vivo lamina propria lymphocytes were cultured for 20 h at 37°C in complete RPMI with IL-23 (25 ng ml−1 R&D Systems) and IL-1β (20 ng ml−1 Peprotech ...
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bioRxiv - Immunology 2024Quote: ... the GR-β blot was washed 3 times with TBS-T and incubated with anti-rabbit HRP-conjugated secondary IgG (1:2000 dilution; cat. no. HAF008, R&D Systems) in 5% w/v non-fat milk (cat ...
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bioRxiv - Immunology 2024Quote: ... and CCL5 levels in the serum of mock- and MAYV-infected animals using the mouse Luminex XL cytokine assay (bio-techne) and the CCL4/MIP-1 beta DuoSet ELISA kit (Catalog# DY451-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with PBS and incubated with an anti-human CCR6 mouse monoclonal antibody (1:50, R&D Systems, U.S.A.) and an anti-CD4 rabbit monoclonal antibody (1:30 ...
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bioRxiv - Immunology 2024Quote: CCL3 and MPO concentrations were detected in immune cell supernatants after 24 h stimulation using Mouse CCL3/MIP-1 alpha DuoSet ELISA and Mouse Myeloperoxidase DuoSet ELISA kits (both R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 96 well plates were coated with 1 μg/mL of recombinant IL13RA2hFc (R&D Systems, Minneapolis, MN, Cat No. 7147-IR-100) and IL13RA1hFc (R&D Systems ...
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bioRxiv - Immunology 2024Quote: Calu-3 or A549 cells were treated with 10 or 100 ng/mL of recombinant human IL-29/IFN-lambda 1 Protein (R&D Systems) for 6 or 24 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...