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Citations for R&D Systems :
3551 - 3600 of 3903 citations for 7 Oxabicyclo 4.1.0 hept 3 ene 2 5 dione 3 hydroxymethyl 4 1E 1 penten 1 yl 1S 6R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2021Quote: ... embryoid bodies were plated on Matrigel (0.17mg/mL in DMEM/F12) in NIM with 10uM BMP-4 (R&D Systems #314BP010). At day 16 ...
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bioRxiv - Pathology 2020Quote: ... 4°C) and plasma was analyzed for TNF-α and IL-10 (porcine specific ELISA kit, R&D Systems, Abingdon, UK). For qPCR ...
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Immunology 2022Quote: ... CFSE-labelled cells were then resuspended in R10 media supplemented with recombinant IL-2 (R&D system) and 2-mercaptoethanol (Themo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... for 72 hours prior to stimulation with 10 ng/mL IFN-α (R&D Systems, 11101-2). Media was prepared by adding 120 μL of 10 mg/mL stock 13C11 15N2 L-tryptophan in 1x PBS to 240 mL of RPMI (Corning ...
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bioRxiv - Immunology 2021Quote: ... and 2.5 ng/ml recombinant mouse IL-15 and 100 units of IL-2 (R&D Systems).
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bioRxiv - Bioengineering 2021Quote: ... ELISA kits for FGF-2 and TGF-β1 were purchased from R&D Systems (Minneapolis, MN, USA) while the ELISA kit for Nodal was obtained from Abcam (Cambridge ...
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bioRxiv - Cell Biology 2020Quote: Supernatant levels of BMP2 were quantified using a BMP-2 Quantikine ELISA kit (R&D Systems #DBP200), without significant deviations from manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... Each treatment infusion was 2 μL of PBS or leptin (10μg/2μL, R&D Systems, Minneapolis, MN). Data collected 30 minutes prior and 30 minutes after the ICV injections were excluded from the analysis ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Immunology 2022Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems) was determined by injecting four concentrations of SARS-CoV-2 Spike (25 nM highest concentration ...
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bioRxiv - Cell Biology 2022Quote: ... mice were given intra-articular injections of recombinant mouse R-spondin 2 (500 ng, R&D Systems) or vehicle (phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2021Quote: ... a standard direct ELISA was performed with DuoSet Ancillary Reagent Kit 2 (R&D systems, Minneapolis, MN). After coating wells with recombinant human EGFR (1μg/mL) ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated for 2 h with human E-cadherin-Fc (R&D system, Minneapolis, MN) at 1 μg / cm2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed every 2 days using differentiation media supplemented with 100ng/ml VEGF (R&D Systems) and 50ng/ml bFGF (R&D Systems) ...
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bioRxiv - Genetics 2023Quote: ... and receptor activator of nuclear factor kappa-B ligand (RANKL) (2 ng/mL; R&D systems; USA) to induce osteoclastogenesis ...
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bioRxiv - Immunology 2023Quote: ... LCN2 in the clear supernatants was quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
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bioRxiv - Biochemistry 2023Quote: The human ADGF (Recombinant Human Adenosine deaminase 2/CECR1) #7518-AD-010 was from R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2019Quote: ... Levels of IL-1β and TNF-α in 200 μl culture medium were measured by ELISA kits according to manufacturer’s instructions (DY401 for IL-1 β and DY410 for TNF-α, R&D systems, Minneapolis, MN, USA). Cleavage of pro-IL-1β and pro-caspase-1 was assessed by western blot of 50 μg of protein extracts of cell lysates ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... cells were plated at 1 million cells per well in 2mL mESC media (without LIF) + 25 μg/L Activin A (R&D Systems 338-AC-050/CF), 5 μg/L BMP4 (PeptroTech 315-27) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were starved in 0.1% FBS for 24 hours prior to addition of EGF (R&D systems #236-EG, diluted in 1 mg/ml BSA/PBS) to 25 ng/μl or carrier alone for 5 min at 37ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% NaN3 in D-PBS) containing optimal concentration determined by antibody titration of the Alexa Fluor® 647-conjugated IGDCC3 extracellular antibody (1:20; R&D Systems; Cat. No. FAB8559R). After a 30 min incubation in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Genetics 2024Quote: The concentration of IGF-1 in the liver was quantified using the Mouse/Rat IGF-I/IGF1 Quantikine ELISA Kit (MG100, R&D Systems, Inc., Minneapolis, MN, USA). Liver protein lysates were diluted at a ratio of 1:6 in calibrator diluent and the assay was performed following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Biochemistry 2024Quote: Recombinantly expressed TIM-4 in HEK293 cells (Q96H15) and podocalyxin-like protein 1 recombinantly expressed in NS0 cells (AAB61574.1) were purchased from R&D Systems (9407-TM, 1658-PD-050). Recombinantly expressed LAMP-4 in HEK293 cells (P34810 ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...