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Citations for R&D Systems :
3351 - 3400 of 3903 citations for 7 Oxabicyclo 4.1.0 hept 3 ene 2 5 dione 3 hydroxymethyl 4 1E 1 penten 1 yl 1S 6R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... NK cells were activated for 72h in 300IU/ml rhIL-2 (R&D Systems). CD4 T cells were simultaneously activated and infected as described above ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant human IL-2 (50 U/mL, R&D Systems, Minneapolis, MN, USA). Single cell suspensions were activated with 1 μg/ml Concavalin A (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% 2-Mercaptoethanol and 10 ng/mL basic FGF (R&D Systems, 233-FB). For MyoD+F/R/C condition ...
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bioRxiv - Bioengineering 2021Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL). After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems). For the TGFβ2 assay ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 30 U/mL recombinant human IL-2 (R&D Systems #202-IL), and 55 μM beta- mercaptoethanol (Thermo # 21985023) ...
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bioRxiv - Developmental Biology 2019Quote: ... After 2 days EBs were treated with 100 ng/ml Noggin (R&D Systems) and 10 mM SB431542 (Tocris ...
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bioRxiv - Immunology 2019Quote: ... IL-1β (PBMC: 2 ng/ml, whole blood: 20 ng/ml, R&D Systems), (5 ...
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bioRxiv - Immunology 2019Quote: ... overnight before addition of 500 IU/ml recombinant human IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... MN) in combination with hrIFNβ at 2 ng/ml (R&D Systems, Minneapolis, MN) or when indicated hrISG15 at 0.115 μg/ml (Biotechne ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-2 (902-MP-010) was purchased from R&D systems (USA). Recombinant human MMP-3 (SRP7783 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems). For the TGFβ2 assay ...
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bioRxiv - Pathology 2021Quote: ... Samples were assayed into a Human ACE-2 DuoSet ELISA assay (R&D systems), loading equal amounts of protein in duplicate for all samples.
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bioRxiv - Immunology 2021Quote: ... Recombinant SARS-CoV-2 Spike glycoprotein was purchased from R&D Systems (Minneapolis, MN). Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV, R&D Systems), MERS-CoV S GCN4-IZ-2P (R&D Systems) ...
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bioRxiv - Bioengineering 2022Quote: ... or 150 ng/ml recombinant human LOX homolog like 2 (LOXL2, R&D Systems), with media changed every 2-3 days ...
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bioRxiv - Immunology 2022Quote: ... 200 IU/ml of recombinant human IL-2 (R&D Systems, 202-IL-050) and expanded for 10 – 14 days ...
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bioRxiv - Neuroscience 2022Quote: ... and 100 ng/mL OX-2 membrane glycoprotein (CD200) (R&D systems, cat.no. 27712CD050) growth factors in addition to the previously described growth factors.
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bioRxiv - Neuroscience 2024Quote: ... and proximal colon and plasma lipocalin 2 (LCN2) (#MLCN20, R&D Systems Minneapolis, MN) were also performed according with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 μM of dynole31-2 (negative control) (R&D Systems, Minneapolis, MN, USA) before or after illumination ...
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bioRxiv - Neuroscience 2023Quote: ... Media was supplemented with 2% fetal bovine serum (FBS) (R&D Systems, Minneapolis, MN), 1% penicillin-streptomycin 10,000U/mL (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... we used the Mouse IL-2 DuoSet ELISA kit (R&D Systems; DY402-05) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 20 ng/mL of recombinant mouse interleukin (IL)- 2 (R&D systems), 8% CTS Immune Cell Serum Replacement (Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng/mL TGFb1 and 2-5ng/ml TGFb2 (both from R&D Systems), 20ng/ml CX3CL1 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng/mL glial cell-derived neurotrophic factor (GDNF, R&D Systems #212-GD), 500 µg/mL dibutyryl cAMP (Sigma #D0627) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Bioengineering 2021Quote: ... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... A 1:6 series of free-floating tissue was stained for TH (MAB318, MilliporeSigma, Burlington, MA) Nurr1 (AF2156, R&D Systems, Minneapolis, MN) or GFP (AB290 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 ug/ml Doxycycline and 3.34 ug/ml Puromycin, 1 × N27 supplement, and 10 ng/ml BDNF (STEMCELL, 78005) and GDNF (R&D Systems, 212-GD)) ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2019Quote: ... were cut on a cryostat and mounted on slides and processed for GDNF receptor GFRα1 (goat anti-GFRα1, 1:100; cat no. AF-560, R&D Systems, Minneapolis, MN, USA) and P2X5 (rabbit anti-P2X5 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
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bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies and dilutions for human muscle cross section imaging: anti-human PDGFR alpha antibody 1:100 (R&D Systems, #AF-307-NA), anti-Laminin 2 alpha 1:250 (Abcam ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Cell Biology 2024Quote: ... Total VEGFR2 expression (0.1% Tx-100 permeabilized cells) was compared to surface level VEGFR2 (non-permeabilized cells) using a biotinylated VEGFR2 antibody (R&D Systems; BT10543). Images were collected using a W1 spinning disk confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serum levels of cytokines (IL-6, TNF-α, MCP-1 and IL-10) were measured by corresponding ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Genomics 2024Quote: ... Progenitors were then resuspended in a 10% dilution in volume of FBS and 100ng/mL CSF-1 (R&D Systems; 216-MC-500) in Gibco™ RPMI-1640 media (Thermo Fisher Scientific ...