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Citations for R&D Systems :
3551 - 3600 of 4278 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: We cultured 3*105 million freshly enriched DCs in 2ml Dendritic Cell Base Media (R&D systems) plus 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... which was supplemented with 3 ng/mL human activin A (R&D Systems, Inc., Minneapolis, MN, USA), 1 ng/mL human bone morphogenetic protein 4 (Bmp4 ...
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bioRxiv - Immunology 2023Quote: ... or with 10 µl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693) ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 mM HMGB1(R&D systems) diluted with protein block buffer (Dako ...
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bioRxiv - Bioengineering 2024Quote: ... The FL5.12 cell culture media was also supplemented with 100 ng/ml IL-3 (R&D Systems). L1210 cells were obtained from ATCC ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... co-housed 8-week-old female C57BL/6J wildtype mice were administered either 300 μg/kg TNF (R&D Systems Cat#410-MT/CF Lot CS152103) in endotoxin-free DPBS or endotoxin-free DPBS alone (Merck Cat# TMS-012-A ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... PBMCs were stimulated for 2 days with anti-CD3/anti-CD28 T cell activation beads (Thermo, catalog no. 11131D) in the presence of interleukin-2 (IL-2; 40 IU/ml; R&D Systems, catalog no. 202-IL-010) in complete T cell media and incubated at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
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bioRxiv - Microbiology 2021Quote: The secreted TNF-α and IL-6 cytokines in the culture supernatants were quantified by ELISA using Duo Set ELISA (R&D systems) as per the manufactures instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... condition media was collected and estimated quantity of the secretary IL-6 using commercially available ELISA kit (R&D Systems, DY 206-05) with human IL6 antibody (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and hESCs were seeded at 3×105 cells/well onto 6-well plates and rested for 24hrs prior to stimulation with PBS or rhIL-23 (1290-IL-010, R&D Systems, USA) at concentrations of 1 ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Cell Biology 2024Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used were as follows: anti-IL-1RA (1: 2000, AF-280-NA, R&D Systems, Minneapolis, USA), anti-p16Ink4a (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were: goat anti-IL-1RA IgG (1: 500, AF-280-NA, R&D Systems, Minneapolis, USA), mouse anti-γH2AX IgG (1:200 ...
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bioRxiv - Immunology 2021Quote: ... E-Selectin and Intercellular Adhesion Molecule (ICAM)-1 were both measured using ELISA assays (R&D Systems, Minneapolis, MN). Lot-to-lot changes in both E-Selectin and ICAM-1 assays were made throughout the course of the study and adjusted prior to the data being made publicly available to the public ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5 µm sections from TMAs or whole blocks were used for ARX (1:1500, R&D Systems, sheep, AF7068), PDX1 (1:100 ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... UK); the antibody against CRLF2 (Cat.#AF981; WB 1 µg/mL) was purchased from R&D Systems (Minneapolis, US). The primary antibodies against pan-RAS (Cat.#8832 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ELISA was performed using the Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The following antibodies were used in this study: goat polyclonal anti-legumain (R&D Systems AF2199; 1:2,000 dilution), mouse monoclonal anti-GAPDH (VWR 1E6D9 ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated overnight on a platform shaker with primary antibodies for ACE2 (1:200, R&D Systems, AF933) and GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with 10 ng ml−1 of recombinant human IFNγ (R&D Systems, 285-IF-100), 10 ng ml−1 of recombinant human TNFα (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... F4/80- cells were sorted and labelled with CD11c-PE and CD11b-FITC antibodies (1:25; R&D Systems) and APC-conjugated Annexin-V (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ELISA was performed using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems DY401-05), using additional reagents from the DuoSet ELISA ancillary reagent kit 2 (R&D Systems DY008) ...
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bioRxiv - Immunology 2021Quote: ... Membranes were incubated with horseradish peroxidase conjugated anti-mouse or anti-rabbit antibodies (1/1000, R&D Systems, USA) in TBS-T (0.1% ...
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bioRxiv - Immunology 2020Quote: ... and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems, 1:500 v/v in blocking buffer) to stain for the cytoskeleton and DAP10 clusters respectively ...
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Immunology 2020Quote: ... in 0.05M HCl for 1 hour at room temperature or [2.5 µg/cm2] mouse E-cadherin (R&D Systems) overnight at 4°C in DPBS ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Immunology 2022Quote: ... and: (1) subjected to multiplex cytokine arrays using Proteome Profiler Mouse Cytokine Array Kit (R&D Systems, Minneapolis, MN); and (2 ...
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bioRxiv - Immunology 2022Quote: ... were used for labeling the epithelium and unconjugated goat anti-mouse CD31 (R&D Systems, AF3628, dilution 1:100) in combination with secondary donkey anti-goat-AlexaFluor555 (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Media were replaced with DMEM containing 1% FBS and treatments of ± 0.4 μg/mL SHH ligand (R&D Systems) ± 100 nM vismodegib (LC Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed and incubated with an HRP conjugated rabbit anti-mouse antibody (1:5000 diluted, R&D systems) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: CH12 cells were cultured for 72h in the presence of TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... anti-CD28 (1 µg/ml, clone CD28.2, eBioscience) and immobilized recombinant ICAM1-Fc fusion protein (0.5µg/ml, R&D Systems) in complete RPMI/HS medium ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were treated with the indicated neuropilin 1 ligands Semaphorin 3A (3nM; Cat#5926-S3-025, R&D Systems), VEGF-A165 (1nM ...
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bioRxiv - Neuroscience 2022Quote: Dissociated commissural neurons were stimulated with 0.1 µg/ml BSA or Netrin-1 (R&D Systems, # 1109-N1-025) for 5 or 30 min and fixed with 4% PFA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Gradients were generated in the Dunn chamber with 200 ng/ml Netrin-1 (R&D Systems, # 1109-N1-025) in the outer well ...
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bioRxiv - Biophysics 2022Quote: ... the gels were washed twice with 1xPBS and incubated at room temperature with a drop of 25μL of 0.01mg·mL-1 histidine- tagged E-cadherin (8505-EC-050, R&D Systems) covered with rectangles of parafilm ...
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bioRxiv - Synthetic Biology 2022Quote: ... membranes were probed with both an anti–6x-His tag antibody (R&D Systems, Cat # MAB050; diluted 1:7,500) and anti–ETEC O148 antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and incubated with an anti-mouse HRP-conjugated secondary antibody (R&D Systems; 1:1000) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Neuroscience 2022Quote: ... Then 1 ml of fresh media was supplemented with IL-34 (R&D systems, cat. No. 5265-IL-010), MCSF (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The neural medium was supplemented with epidermal growth factor (EGF, 20 ng ml-1, R&D Systems, 236-EG) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...