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Citations for R&D Systems :
3801 - 3850 of 4278 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Calprotectin levels were determined in optimally diluted (1:20) extracts using Human-S100A8/S100A9 Heterodimer DuoSet-ELISA kit from R&D Systems. Zonulin levels were determined in similarly diluted extracts using human Zonulin Stool ELISA kit from ALPCO ...
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bioRxiv - Biophysics 2022Quote: Endogenous CXCR4 expression on the surface of HEK and U87 cells was monitored by flow cytometry using CXCR4-specific phycoerythrin-conjugated mAb 12G5 or the corresponding isotype control (1:20, R&D Systems) in a BD FACS LSR Fortessa cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Human CSF-1 and IL-34 human serum levels were quantified using ELISA kits (both R&D systems, Mountain View, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Immunology 2020Quote: TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fed daily with different cocktails of growth factors and small molecules for twelve days in mesodermal differentiation medium and driven through the anterior primitive streak (1 day; 30 ng/ml Activin [cat. num. 338-AC; R&D Systems, Minneapolis ...
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bioRxiv - Cell Biology 2021Quote: ... sections were blocked with 10% rabbit serum for 15min at room temperature and then incubated with goat anti mouse IGF1(1:40, AF791, R&D System) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were separately preincubated with neuronal cultures and recombinant tau protein for 3 hours prior to incubation of neuronal cultures with the mixer containing recombinant proteins (100nM Human LRP-1 Cluster IV Fc Chimera Protein; 10nM Human LRPAP Protein [R&D systems]). For pharmacological treatments ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified by using the Mouse TGFβ 1 DuoSet ELISA (#DY1679-05, R&D systems), complemented with Sample Activation Kit 1 (#DY010 ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Incubation with primary rat-polyclonal antibody against LYVE-1 in combination with rat-polyclonal biotinylated anti-MHC-II antibody (both R&D Systems) was done for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1h at room temperature and probed with primary antibodies against SORCS2 (1:750; AF4238, R&D Systems) and GAPDH (1:10,000 ...
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bioRxiv - Cancer Biology 2021Quote: CCL2 and CXCL1 were also quantified with Mouse CCL2/JE/MCP-1 and Mouse CXCL1/KC DuoSet ELISA kits (R&D Systems), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Cancer Biology 2020Quote: ... After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution, 865006, R&D Systems) for 90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
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bioRxiv - Immunology 2020Quote: Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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bioRxiv - Biochemistry 2021Quote: ... Selective isolation of MLC1-complex from the PM was achieved by cs-IP using anti-GlialCAM Ab (1:2000, R&D Systems). Ab was bound on ice to live cells for 45min ...
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bioRxiv - Microbiology 2021Quote: ... and 10μM SB431542 for 24 hours followed by switching to SFD media supplemented with 10 μM SB431542 and 1 μM IWP2 (R&D Systems) for another 24 hours ...
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bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... eluted products and 10% of the input were separated by SDS-PAGE and transferred to a PVDF membrane for blotting at 4C with Gli3 (polyclonal goat IgG 1:1000, R&D Systems) and Hand2 (polyclonal goat IgG or mouse monoclonal IgG1 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40; AF3075; R&D Systems) and rabbit polyclonal antibodies against GFAP (1:250 ...
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bioRxiv - Immunology 2021Quote: ... followed by 1 hour incubation in 3% nonfat milk in Tris-buffered saline with 0.1% Tween 20 detergent (TBST) buffer with horseradish peroxidase-conjugated anti-rabbit (1:2000, R&D Systems, HAF008) or mouse (1:2000 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1 % Triton-X-100 in PB (0.1 mol/L, pH 7.4) and incubated with goat anti-CD31 (1:100, R&D systems, Minneapolis, USA). As secondary antibody biotinylated anti-goat IgG (1:500 ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...
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bioRxiv - Molecular Biology 2024Quote: ... ELISAs were performed to quantify IL-1β in these culture supernatants using the Mouse IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) per the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Total THP levels of a subset of urine samples (diluted 1:10) was determined by ELISA (catalog no. DY5144-05, R&D Systems) per manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: Cells in 60 wells were incubated with primary goat polyclonal anti-mouse E-cadherin (R&D Systems, Cat. No AF748, 1:200) in cold 5% FBS/PBS for 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... treatment wells received 10 ng/ml (for migration assays, co-culture assays proteomic screening) and or 1 ng/ml (for RNA-seq) of rhIFNγ (R&D systems).
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150 ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of the supernatant was diluted with 25 μl 1% BSA PBS-T and added to a Nunc Maxisorp plate coated with 1 μg/ml anti-C5a capture antibody (C5a DuoSet ELISA kit, R&D systems). Next ...
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bioRxiv - Immunology 2023Quote: ... Levels of IL1β protein were determined in culture supernatant using the IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Molecular Biology 2022Quote: ... Lot #: 413-ML-005) and gremlin 1 (Cat #: 956-GR-050, Lot #: ESD4616121) recombinant proteins were all purchased from R&D Systems. Gusb ...
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bioRxiv - Microbiology 2023Quote: ... and the medium was removed and washed with 1× PBS before viral infection and TGF-β1 (catalog #240-B-002, R&D Systems) treatment ...
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bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...