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Citations for R&D Systems :
251 - 287 of 287 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived P16T colorectal cancer organoids (Van de Wetering et al., 2015) were cultured in Basement Membrane Extract (BME, R&D systems) drops in culture medium containing advanced DMEM/F12 medium containing 10 mM HEPES (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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bioRxiv - Genetics 2022Quote: ... Membranes were probed by incubation for 1 hour at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592 ...
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Each human plasma sample was loaded independently on a membrane of the Proteome Profiler Human XL Cytokine Array (ARY022B, R&D systems) following the manufacturer’s recommendations and background noise removal was performed identically.
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bioRxiv - Cell Biology 2024Quote: Organoid lines were obtained as mentioned below (organoid lines) and maintained in corresponding media (see table 1) and Basement Membrane Extract (BME, R&D systems) BME®-domes ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Genetics 2023Quote: Generated hiPSC lines were layered onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Genetics 2023Quote: Fully differentiated Day 30 Cardiomyocytes were resuspended onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Systems Biology 2024Quote: ... were generated at the Institute of Precision Medicine (Pitt/UPMC) and cultured in 40 µL of Cultrex Basement Membrane Extract (Cat. 343200101, R&D Systems™) in 24 well plate in organoid medium (recipe and culture procedures in ...
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bioRxiv - Genetics 2023Quote: ... and colonies were picked into a Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 24-well plate for expansion ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mice (6-8 weeks old) were injected with 1x106 cells suspended in PBS and Cultrex™ basement membrane extracts (BME) (Bio-techne/R&D Systems) into the right flank ...
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bioRxiv - Synthetic Biology 2024Quote: ... 600 µg of protein was used to load the membranes of the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, USA). The Assay was performed according to the specifications provided by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: Twenty thousand HUVEC cells were seeded in reduced growth factor basement membrane extract (BME)-coated 96-well plate (R&D systems: 3470-096-K). Endothelial cells were exposed to 100 μl of the different benign or malignant cells conditioned media ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein was applied to each ELISA membrane and developed according to the manufacturer’s instructions (Proteome Profiler Mouse XL Cytokine Kit, ARY028, R&D Systems, Minneapolis, MN, USA). Cytokine array signals were detected by X-ray film ...
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bioRxiv - Cancer Biology 2020Quote: ... were used for this study.1.8×106 22Rv1 cells stably transduced with doxycycline-inducible shScramble or shTMEFF2-9 shRNAs and mixed with Basement membrane extract (R&D systems, Minneapolis, MN) at a 1:1 ratio ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were grown in their undifferentiated form on 20ug/mL Cultrex reduced growth factor basement membrane (catalog number 3433, R&D Systems, Minneapolis, Minnesota, USA) in DMEM/F12 (catalog number 11320033 ...
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bioRxiv - Bioengineering 2024Quote: ... was used as a hydrogel matrix for BxPC3 cell culture and Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, BME001-05) were used as the hydrogel matrix for A549 ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Transparent PET membrane culture inserts of 24-well plate (Falcon, 353097) were coated with Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, 3533-010-02) at 50μg per membrane (200μl of 0.25mg/ml BME stock per membrane ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2024Quote: ... mice received intra-ceacum injection of 1.5 x 105 AKPS cells in 10 uL basement-membrane extract (BME, R&D Systems Cat # 35-330-0502) and tumors were analyzed six weeks post-injection ...
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bioRxiv - Molecular Biology 2023Quote: Crypts isolated from mouse intestine were seeded at a density of 250 crypts per 25 µL Matrigel (Cultrex reduced growth factor basement membrane extract, type R1, R&D Systems, Catalog #3433-010-R1) in 48 well plates ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 M/mL PyMT tumor cells were suspended in collagen +/-L-ribose supplemented with basement membrane extract [20 % v/v final (R&D Systems, Cultrex BME, 3532-005-02) and DMEM [1x final] and the pH was adjusted to ∼7.3 ...