Labshake search
Citations for R&D Systems :
151 - 200 of 287 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well) in the presence of 20 ng/mL M-CSF (216-MC-005; R&D Systems) for 4 days.6 Monocyte-derived macrophages [(Mb) ...
-
bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
-
The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... Cells were plated and cultured in complete medium containing 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems). The medium was refreshed every 3 days ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... with or without 1.25 µg/mL Scl-Ab or 500 ng/mL anti-M-CSF antibody (#AF416 from R&D Systems). After 8 days ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
-
bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
-
bioRxiv - Microbiology 2020Quote: ... Fully-differentiated MDM were generated by culturing MC under adherent conditions for seven days using RPMI-AS medium supplemented with 10 ng/mL M-CSF (R&D Systems). T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
-
bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
-
bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
-
bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
-
bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
-
bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
-
bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
-
bioRxiv - Cancer Biology 2023Quote: ... Osteoclast differentiation media consisted of standard OCY454 media supplemented with 25ng/ml M-CSF (R&D Systems Catelog # 416-TEC; Lot # ME5021121) and 5 ng/ml RANKL (R&D Systems Catalog #462-TEC-010 ...
-
bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
-
bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Molecular Biology 2023Quote: ... over the course of 7 additional days with 25 ng/mL M-CSF and 25 ng/mL RANKL (both from R&D Systems), as previously described [17,30,49] ...
-
bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
-
bioRxiv - Bioengineering 2024Quote: ... then differentiated into M0 macrophage with 25 ng/ml recombinant macrophage colony-stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA) for 4 days ...
-
bioRxiv - Cancer Biology 2023Quote: A membrane-based antibody array (Proteome Profiler Human Cytokine Array Kit, R&D Systems, ARY005B) was used to profile 36 soluble proteins ...
-
bioRxiv - Cancer Biology 2023Quote: ... or Cultrex Basement Membrane Extract (BME Type II or Ultimatrix RGF BME, R&D Systems) and dispensed onto 24-well plates (Corning) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The membrane was incubated with anti-Mouse SOST/Sclerostin (R&D System, AF1589, 1:500) antibody in Bullet Blocking One (Nacalai Tesque ...
-
bioRxiv - Immunology 2024Quote: ... Following membrane stripping goat anti-human/mouse serum albumin (R&D Systems Inc, Minneapolis, MN) at 1:2500 was used as the primary Ab ...
-
bioRxiv - Cell Biology 2024Quote: ... Keeping the Cultrex (Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, R&D Systems) on ice ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in complete αMEM with 40 ng/ml M-CSF (ProSpec-Tany TechnoGene, Rehovat, Israel) and l0 ng/ml RANKL (R&D Systems, MN). Tartrate-resistant acid phosphatase (TRAcP ...
-
bioRxiv - Pathology 2024Quote: ... the BMMs are incubated for three days in a 24-well plate with medium supplemented with 30 ng/ml M-CSF and 50 ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D Systems, MN). After this period ...
-
bioRxiv - Microbiology 2021Quote: ... the membrane was incubated with goat pAB anti-hACE2-700 (1:200, FAB933N R&D Systems) and mouse mAB anti-b-actin (1:5000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Basement membrane extract (BME, 1X; 100 μL; cat. no. 3455-096-02, R&D systems, rndsystems.com) was mixed with coating solution (1X ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were resuspended in 70% basement membrane extract (BME) (R&D Systems, #3533-010-02). Each droplet of cell clusters contained roughly ∼2,000 cells/ 50uL ...
-
bioRxiv - Immunology 2023Quote: ... After incubation with nitrocellulose membranes from the Human XL Cytokine Array kit (ARY022B, R&D Systems), the cytokine/ detection antibody complexes were bound by their cognate capturing antibodies immobilized on the membranes as spots ...
-
HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... The membranes were probed with an anti-MHC antibody (1:100, R&D Systems, Cat.# MAB4470) in blocking buffer (5% BSA ...
-
bioRxiv - Genomics 2023Quote: ... membranes were probed overnight at 4°C with antibodies that recognize ERAP2 (AF3830, R&D Systems) or α-tubulin (T9026 ...
-
bioRxiv - Cancer Biology 2024Quote: 3D spheroids were established using non-diluted reconstituted basement membrane (rBM; Cultrex™; R&D Systems) on acid-washed glass coverslips ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... resuspended in N2-S medium supplemented with 20 ng/ml EGF and 20 ng/ml FGF2 (both from R&D Systems, Minneapolis, M USA) and seeded as droplets (10 µl ...
-
bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
-
bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
-
bioRxiv - Neuroscience 2021Quote: Differentiated BMDM were plated in 96-well plate at density of 30,000 cells/well either in the presence of 40ng/ml M-CSF (R&D systems, 416-ML-050) or without M-CSF ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in the presence of 50 ng/mL of GM-CSF (02532, Stem Cells, Vancouver, Canada) and 50 ng/mL of M-CSF (216-MC, R&D Systems, Minneapolis, USA) at 37°C and 5% CO2 for differentiation ...
-
bioRxiv - Cell Biology 2023Quote: ... non-adherent hematopoietic progenitors were pooled and plated at 7x104 cell/well (96-well) with complete media supplemented with 30 ng/mL M-CSF (R&D Systems, 416-ML). At 90% confluence ...
-
bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
-
bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated with a mouse anti-zebrafish VEGF antibody (R&D Systems #MAB1247, 1:1,000) and chicken anti-GAPDH antibody (Sigma #AB2302 ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN) overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
-
bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-coupled anti-mouse immunoglobulin (R&D System, Minneapolis, MN, USA) at room temperature for 1 h and after subsequent washes ...
-
bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-coupled anti-mouse immunoglobulin (R&D System, Minneapolis, MN, USA) at room temperature for 1 h ...
-
bioRxiv - Cell Biology 2020Quote: ... cell lysates were incubated overnight with nitrocellulose membranes of the Human Phospho-Kinase Array (R&D Systems). Membranes were then washed ...
-
bioRxiv - Physiology 2023Quote: ... The pellet was resuspended in the appropriate amount of Cultrex Basement Membrane Extract (353300102, R&D Systems). 50 μL tabs were plated onto 6 well-plates (FB012927 ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were probed using goat anti-mouse LDLR at a 1:500 dilution (AF2255; R&D Systems) or goat anti-human LDLR at a 1:500 dilution (AF2148 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Organoids were maintained in CultrexTM UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-10, R&D Systems) and incubated in Advanced DMEM/F12 (12-634-010 ...