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Citations for R&D Systems :
2751 - 2794 of 2794 citations for Recombinant Mouse IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The levels of CXCL1 in BALF and TNFα in serum were determined using Quantikine® mouse ELISA kits (R&D systems, Inc., Minneapolis, MN, USA). The Candida β-glucan concentration in BALF or serum was determined with Glucatell kits (Associates of Cape Cod ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Cell Biology 2022Quote: Intracellular cAMP expression levels were measured after 10min to 48h of PTH treatment using Mouse/Rat cAMP Assay Parameter™ Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions in both pre-differentiation COBs and BMSCs ...
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were analyzed by SDS-PAGE and immunoblot using an anti-histidine tag antibody (Monoclonal Mouse IgG1 histidine tag antibody; R&D Systems, cat. no. MAB050). Fractions containing TDO2 were combined and dialyzed (molecular weight cutoff 3.5 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: The cytokine/chemokine profile of mice plasma or culture supernatants was determined by using proteome profiler mouse cytokine array kit (R&D Systems, CAT. No. ARY006). The expression levels of cytokine/chemokine were measured by pixel density of each dot using the ImageJ software ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cytochrome c concentrations in the isolated cytosolic fraction were measured by rat/mouse cytochrome c quantikine ELISA Kit (MCTC0, R&D Systems, Inc., Minneapolis, MN).
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels within the lung and brain homogenates of infected mice were analyzed using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems Inc., Minneapolis, MN). The relative expression levels of 111 soluble mouse proteins were determined following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ splenic T-lymphocytes were polarized to IL-17A-producing phenotypes ex vivo by use of CellXVivo Mouse TH17 Cell Differentiation Kit (#CDK017, R&D Systems, Minneapolis, MN, USA). Briefly ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... there were six mice per treatment and control group for a total of 78 mice in thirteen groups: (A) Seven vaccine groups included 1 μg of unformulated SARS-CoV-2 spike protein (R&D Systems, Cat# 10549-CV-100) delivered with adjuvanted NPs ...
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bioRxiv - Cell Biology 2019Quote: Paraffin-embedded specimens of brown adipose tissue (BAT) were cut and immunostained with antibody to uncoupling protein-1 UCP-1 (R&D Systems, cat. # MAB6158, Minneapolis, MN) at a concentration of 10 µg/mL by personnel of the Experimental Pathology Core of NYU School of Medicine ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Recombinantly expressed TIM-4 in HEK293 cells (Q96H15) and podocalyxin-like protein 1 recombinantly expressed in NS0 cells (AAB61574.1) were purchased from R&D Systems (9407-TM, 1658-PD-050). Recombinantly expressed LAMP-4 in HEK293 cells (P34810 ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... the IGF-1 concentration in the medium was measured using the Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA)
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... 1.5 mL of medium from each condition was collected for cytokine analysis and relative intensity of a panel of cytokines was measured (R&D Systems, Mouse Cytokine Array Kit).
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Immunology 2022Quote: Extracellular calprotectin was measured in plasma and culture media using the Mouse S100a8/S100a9 Heterodimer DuoSet Solid Phase Sandwich ELISA Kit (R&D Systems #DY859605, Minneapolis, MN, USA) as per manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... #CCL-131) were plated in each well of 96-well plates and preactivated with 100 ng/mL mouse IFN-γ (R&D Systems, #485-MI-100) for 18-24 hours ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Genetics 2024Quote: The concentration of IGF-1 in the liver was quantified using the Mouse/Rat IGF-I/IGF1 Quantikine ELISA Kit (MG100, R&D Systems, Inc., Minneapolis, MN, USA). Liver protein lysates were diluted at a ratio of 1:6 in calibrator diluent and the assay was performed following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Immunology 2023Quote: ... the pellets obtained after centrifugation of the BAL fluid were pooled and stained for 30 min at 4°C with unconjugated polyclonal goat anti-mouse Ym1/2 (AF2446, R&D Systems; 1:100 diluted in PBS). After washing ...
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bioRxiv - Physiology 2024Quote: ... Protein expression was visualised using immunofluorescence staining of mesenteric artery rings and the following antibodies: anti-CD31 (PECAM, # AF3628, R&D Systems, 1:1000 dilution, raised in goat); anti-TRPV4R (# ACC-034 ...
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...