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Citations for R&D Systems :
2451 - 2500 of 2794 citations for Recombinant Mouse IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 30 µL (50 µg protein) lysate with 70 µL of Buffer A buffer containing 20μM Suc-LLVY-AMC (R&D Systems). Assay parameters ...
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bioRxiv - Immunology 2021Quote: ... After stimulation wells were washed and incubated with a biotinylated anti-IFNγ antibody (R&D systems, mouse IFNγ Kit Cat # EL485) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were trypsinized and incubated with APC-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Bioengineering 2019Quote: ... Blots of spleen lysates were prepared with a Proteome Profiler Mouse Cytokine Array Kit (Panel A, R&D Systems, Minneapolis, MN) using an established protocol.[23] After blot development ...
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bioRxiv - Neuroscience 2019Quote: Antibodies and dilutions used for immunofluorescence staining were as follows: polyclonal goat anti-mouse Pdgfra (R&D Systems, AF1062, 1:500), monoclonal rabbit anti-mouse Olig2 (Abcam EPR2673 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 min at 4°C) pre-incubated with 5 μl TRKB antibody (Goat anti-mouse TRKB Antibody, #AF1494, R&D Systems) and cleared through new Protein-G sepharose tubes ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti-Tuj1 polyclonal antibody (Poly18020, Covance) (1:200),and mouse anti-Tuj1 monoclonal antibody (clone TuJ-1, R&D systems) (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cytokine array were conducted by using a Proteome Profiler Mouse XL Cytokine Array kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatants were analyzed for relative changes in chemokine levels using the Proteome Profiler Mouse Chemokine Array Kit (ARY020, R&D Systems). Results from this array were confirmed using DuoSet ELISA kits from R&D Systems for CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... For ICAM-1 cross-linking experiments on ECs we used mouse anti ICAM-1 (Clone # BBIG-I1) from R&D systems and secondary antibody from donkey (DK) ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag mouse monoclonal antibody (MAB050) and the basic-based antigen unmasking solution (CTS013) from R&D Systems (Minneapolis, MN); anti-FLAG M2 mouse monoclonal antibody (F1804) ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine levels were analyzed by using the Proteome Profiler Mouse Cytokine Array Panel A kit (R&D Systems, Minneapolis, MN, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: For sTREM2 measurements, multi array 96-well plates (MSD, L15XA-3) were coated with mouse anti-TREM2 (R&D systems, AF1729) capture antibody O/N at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Neuroscience 2022Quote: ... Group 1 ligands were derived from mouse transcriptomic data and included: APP (R&D Systems, Cat. 3466-PI-010, 20ng/mL), APOE3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Some of ITGB4−/− mice were intraperitoneally (i.p.) treated with 150 μg anti-mouse CCL17 monoclonal antibody (mAb, clone 110904, R&D Systems) or isotype control mAb (MAB006 ...
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...
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bioRxiv - Microbiology 2019Quote: ... and KC and IL-1β from mouse brain homogenates were detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Immunology 2020Quote: ... The cytokine concentrations in the culture supernatant were determined using quantitative ELISA (Mouse TNF and IL-10 DuoSet; R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... IL-1β levels were measured via a mouse IL-1β ELISA assay according to the manufacturer’s instructions (R&D Systems, MLB00C). Released IL-1β levels were normalized to cellular protein ...
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bioRxiv - Pathology 2020Quote: ... HRP-conjugated mouse anti-IgG (HAF007) and HRP-conjugated goat anti-IgG (HAF017) were purchased from R&D Systems (Minneapolis, USA). Urine protein test kit (C035-2-1) ...
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bioRxiv - Physiology 2020Quote: Fecal lipocalin-2 levels in distal colon fecal contents were analyzed using Mouse Lipocalin-2 DuoSet ELISA (R&D Systems, DY1857) according to manufacturer provided instructions ...
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bioRxiv - Physiology 2020Quote: ... IGF-1 was measured using anti-IGF monoclonal antibody Clone #126002 and anti-mouse IGF-1 Biotinylated Antibody (R&D systems).
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Molecular Biology 2020Quote: Cytokine levels in cell culture supernatants samples were determined using the Proteome Profiler Mouse Cytokine Array Panel A (R&D Systems) following the manufacturer recommendations.
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bioRxiv - Cancer Biology 2020Quote: ... The supernatants were collected and subjected to ELISA measurement of IFNγ using Mouse IFN-gamma Quantikine ELISA Kit (R&D systems).
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bioRxiv - Cell Biology 2021Quote: ... Serum IGF-1 levels were measured by Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA). Serum parameters were measured and calculated with a VITROS analyzer (Ortho Clinical Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: IL-10 and TNFα in serum and CM-MSC were quantified using mouse Quantikine ELISA IL-10 immunoassay (R&D Systems) and TNFα ELISA high sensitivity (eBioscience ...
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bioRxiv - Immunology 2022Quote: The production of 111 cytokines by splenic monocytes was quantified using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems). Cytokine antibody array was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed 3x in 0.1% Triton X-100 in PBS and incubated with the secondary antibodies: anti-mouse IgG2a 647 (Alexa Fluor R&D systems), anti-mouse IgG2a 488 (Alexa Fluor R&D systems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Developmental Biology 2022Quote: IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD) ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...
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bioRxiv - Neuroscience 2023Quote: ELISA kits were used to determine the levels of GDF15 (mouse/rat GDF15 Quantikine ELISA Kit, R&D Systems, catalog # MGD150), TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
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bioRxiv - Cell Biology 2024Quote: The levels of CXCL9 secreted to the media by MIN6 cells were quantified by Mouse DuoSet ELISA kit (R&D Systems). Polystyrene 96-well plates were coated with 100 μL of 200 ng/mL unconjugated goat anti-mouse CXCL9 antibody in PBS at room temperature overnight ...
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bioRxiv - Bioengineering 2024Quote: Purified mouse monoclonal IgG anti-human interferon-γ capture antibody (Cat. #MAB2852, lot #FIO1022021, R&D Systems, Minneapolis, Minnesota, United States), biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 μl of cell culture media was treated and incubated with the Proteome profiler mouse cytokine array membrane (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679-05) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Sections were incubated overnight with unconjugated goat anti-mouse/rat CD47 N-terminal IgV-like extracellular domain antibody (R&D Systems), biotin-conjugated anti-mouse CD55 REAfinity antibody (clone REA300 ...
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bioRxiv - Developmental Biology 2023Quote: Surface expression of ITFG1 on cells was examined by staining the cells with mouse anti-ITFG1 antibody (R&D Systems, MAB89001) at 4°C for 1 hour ...