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Citations for R&D Systems :
2701 - 2750 of 2824 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Mouse cell culture supernatants were assayed using commercially available sandwich ELISA kits for mouse IL6 (R&D Systems, Minneapolis, MN, catalog# M6000B), and TNF-α (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... M-CSF protein levels were determined in bone marrow supernatants (obtained by centrifugation at 1000 g for 10 minutes) or in cell culture medium using Quantikine ELISA kits (R&D Systems). Results obtained with bone marrow supernatants were normalized to total protein levels.
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bioRxiv - Microbiology 2024Quote: ... The UCP conjugates for neural cell adhesion molecule (NCAM/CD56) and serpin-A3 were prepared with goat anti-human NCAM Ab (AF2408, R&D systems) and goat antihuman serpin-A3 (AF1295 ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then rinsed with PBS and chromogenic staining was performed using Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (VCTS003, R&D Systems) following manufacturer’s instructions and counterstained with hematoxylin (Millipore Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... and 10% R-spo1 conditioned medium prepared by culturing Cultrex HA-R-Spondin1-Fc 293T cells (R&D Systems; 3710-001-01) to generate premature ...
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bioRxiv - Cancer Biology 2024Quote: ... one million cells were suspended in 100 µl of 1:1 RPMI and BME-Type 3 (R&D Systems, # 36-320-1002P) per implantation ...
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bioRxiv - Cancer Biology 2024Quote: ... Ba/F3 cell medium was additionally supplemented with 5% WEHI cell-conditioned medium as a source of IL3 while the media for IL3-independent Ba/F3 cells were supplemented or not with neuregulin-1β (NRG-1) (396-HB-050; R&D Systems). MCF10a cell medium was additionally supplemented with 20 ng/ml EGF (AF-100-15 ...
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bioRxiv - Developmental Biology 2024Quote: ... MEF cells were starved overnight and then treated with either FGF2 (50 ng/µl) or PDGFA (20 ng/µl) (from R&D systems) for 5 mins ...
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bioRxiv - Cell Biology 2024Quote: ... the level of the chemokine IL-8 in the basal medium of Caco-2/TC7 cells was quantified using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems).
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bioRxiv - Developmental Biology 2024Quote: ... For hESCs and cyiPSCs 1.25 x 105 cells/cm2 and for mEpiSCs 6.25 x 104 cells/cm2 were seeded onto Matrigel coated plates in UPPS media supplemented with 10 µM Y-27632 (R&D Systems) and incubated overnight ...
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MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasiabioRxiv - Cancer Biology 2021Quote: WT and Myb+/− bone marrow cells stained for the KSL markers were incubated with 5μM Me4bodipyFL-Ahx3Leu3VS probe (R&D Systems, Minneapolis MN) for 1 hour at 37°C either with or without prior incubation for 1 hour at 37°C with 10μM of the proteosome inhibitor MG-132 (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: All cells were grown in a humidified incubator at 37°C and 5% CO2 and cell lines tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems).
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bioRxiv - Cancer Biology 2020Quote: ... Then we collected the supernatants and cell lysates so that we could detect the chemokines using a Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017).
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bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Biochemistry 2021Quote: Acvr1[R206H]/+and Acvr2a −/−;Acvr2b −/−;Bmpr2 −/−;Acvr1[R206H]/+ mES cells were dissociated using non-enzymatic cell dissociation buffer (MiliPore, S-004-B) and resuspended in the flow cytometry staining buffer (R&D systems, FC001). After 15 minutes of blocking (Thermofisher ...
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bioRxiv - Pathology 2020Quote: Osteoprotegerin was measured in culture supernatants of cells and slices using a murine OPG DuoSet® ELISA kit (R&D Systems, Minneapolis, US) according to the instructions provided by the manufacturer.
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Neuroscience 2022Quote: ... 1:500, rabbit anti-PDGFRα (3164 Cell Signalling Technologies, Danvers, Massachusetts) 1:500 and goat anti-Sox10 (AF2864 R&D Systems, Minneapolis, Minnesota) 1:50 ...
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Cancer Biology 2024Quote: ... A time course was performed on cells treated with 300 ng/mL of recombinant mouse VEGF-B186 (R&D Systems #767-VE-010) for 0 ...
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bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells were analyzed using ELISpot according to the manufacturer’s protocol ELISpot Rat interferon-gamma (IFNγ) (Cat: EL585 by R&D Systems, United States).
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bioRxiv - Cancer Biology 2023Quote: ... HUES8 cells were treated with 3μM CHIR99021 (CHIR, Stem-RD, Hoover, AL) and 100 ng/mL Activin A (AA, R&D systems, Minneapolis, MN) in RPMI media (Cellgro ...
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bioRxiv - Cancer Biology 2023Quote: AREG-neutralising experiments were performed by pre-incubating freshly prepared EVs (at 4 x 103 EVs per recipient cell) with either 4 µg/ml of goat anti-AREG (R&D Systems #AF262) or goat anti-IgG (R&D Systems #AB-1080-C ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.
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bioRxiv - Genetics 2023Quote: ... and colonies were picked into a Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 24-well plate for expansion ...
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bioRxiv - Genetics 2023Quote: Generated hiPSC lines were layered onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Genetics 2023Quote: Fully differentiated Day 30 Cardiomyocytes were resuspended onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Cancer Biology 2024Quote: ... mice (6-8 weeks old) were injected with 1x106 cells suspended in PBS and Cultrex™ basement membrane extracts (BME) (Bio-techne/R&D Systems) into the right flank ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with an equal volume of 1 mg/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... was used after viability and extracellular staining and cells were incubated in Perm/Wash buffer with Arginase-1 (APC, R&D systems #IC5868A) for 30 minutes at 4℃ ...
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bioRxiv - Genetics 2024Quote: ... For the QC the cells were plated on Cultrex coated Corning™Costar™96 well plates (R&D systems, #07-200-90) in NIM+10 % CloneR ...
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bioRxiv - Biochemistry 2024Quote: Serum starved RPE1 cells and fibroblasts were stimulated by addition of respective serum starvation media containing 2ng/mL TGFB-1 ligand (R&D Systems #240B) for the specified time points ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
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bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell lines were purchased from ATCC (Manassas, VA, USA) and confirmed to be mycoplasma free by MycoProbe kit (R&D Systems, Minneapolis, MN, USA). Cells were grown at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: Twenty thousand HUVEC cells were seeded in reduced growth factor basement membrane extract (BME)-coated 96-well plate (R&D systems: 3470-096-K). Endothelial cells were exposed to 100 μl of the different benign or malignant cells conditioned media ...