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Citations for R&D Systems :
2601 - 2650 of 2824 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Genomics 2023Quote: ... MDA-MB-231 cells were cultured in DMEM high-glucose medium with 10% FBS (Thermo Fisher Scientific or R&D Systems). Doxycycline-inducible GATA3 expression system in MDA-MB-231 cells was developed by lentiviral transduction ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... Cells were seeded in 96-well plates and 24 hours after plating treated with: THBS1 (R&D systems, 7859-TH-050), syndecan-4 Ab (BD Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: ... Harvested CM was normalized to the fibroblast cell count and assessed for IGFBP2 using ELISA purchased from R&D system (DGB200). Serum samples were diluted 50-fold ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Fixable Viability Dye eFluor™ 780 (65-0865-14) and CD34 (RQR8)-APC (R&D Systems; FAB7227A), washed in PBS and resuspended in BD Cytofix/Cytoperm™ (BD ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... The cell mediums were collected and used to detect the TNFα levels by the TNFα ELISA kit (R&D Systems, MTA00B). All ELISA assays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Cancer Biology 2023Quote: ... We used a Comet Assay Single Cell Gel Electrophoresis Assay kit (cat. no. 4250-050-K; R&D Systems, Bio-Techne) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... about 15.000 cells per well were resuspended in Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (R&D Systems) and seeded in pre-heated low adhesion 24-well plates (GreinerBio) ...
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bioRxiv - Molecular Biology 2023Quote: ... Colony forming assays were performed by plating 200 cells of each isolated subpopulation in Complete Methylcellulose Media (R&D Systems; HSC003).
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bioRxiv - Physiology 2024Quote: HEK293 cells and HEK293 stably transfected with membrane bound KL (HEK293-mKL cells) were cultured in Alpha MEM (SH30265.01; Hyclone, Marlborough, MA) supplemented with 10% fetal bovine serum (S11195; R&D Systems), 1% L-Glutamine 100X Solution (Hyclone) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The D1-HEK cells were transfected overnight and the following morning split into poly-L-lysine (R&D systems, Minneapolis, MN) coated 96-well white clear bottom cell culture plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 mg/ml of purified recombinant human complement component C5a (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... knockout and ALFA-tagged cells were cultured in the presence of 20 ng/ml interferon-γ (R&D Systems 485-MI), treated with DMSO or 1 µg/ml Dox for 24 hours before flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... 5×104 labeled cells were resuspended in B cell media (RPMI, 5%FCS, 50μM 2-mercaptoethanol and 2mM glutamine) and stimulated with 50ng/ml CD40L (R&D Systems), 50ng/ml IL-4 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... and IL-18 were quantified in cell supernatants using the DuoSet ELISA Kit from R&D Systems (DY210, DY201 and DY318).
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... Cells were plated and cultured in complete medium containing 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems). The medium was refreshed every 3 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then added to 1% low melting point agarose at low density (10,000 cells/mL) and 50μL of suspension was added to ice-cold CometSlides (R&D Systems #3042135) on wet ice in a lidded cooler ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at 6×105/cm2 or 3×105/cm2 (corresponding to high or low cell density) on 24-well transwell plates in MEF-CM supplemented with 20 ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Developmental Biology 2021Quote: ... 8×105 cells were seeded on each coverslip in a defined volume of MEF-CM supplemented with 20ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Molecular Biology 2021Quote: ... washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems) according to vendor’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cell suspensions of wild type and Pds5a KO mESCs were permeabilized and fixed using the fixation/permabilization buffer (R&D systems), washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... while 29-13 cells were passaged in Beck’s Medium supplemented with 15% doxycycline-free fetal calf serum (R&D Systems—Minneapolis, MN), 50 μg mL-1 hygromycin (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... The cells were again divided and incubated in the presence or absence of 50 ng/ml TGFβ (R&D Systems, Minneapolis, MN). Cells were harvested at 30 minutes and 24 hours post addition of TGFβ and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Immunology 2020Quote: Levels of human IL-1β (DY201) and IL-18 (DY318) were measured in the cell supernatants using ELISA kits from R&D Systems. Human IL-6 (# 88-7066-86 ...
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bioRxiv - Cancer Biology 2021Quote: ... Intracellular granzyme B and INFγ staining was performed in cells that had fixed and permeabilized in Flow Cytometry Fixation and Permeabilization Buffers (Catalog numbers FC004, FC005, R&D systems) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants were collected and IL-8 secretion was determined using IL-8 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5 × 105 cells in 100 μl adhesion buffer were added to the upper chamber and either 500 ng/ml CCL2 (R&D Systems) or 10 nM C5a (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 24 hours either with vehicle or with 10nM GnRH and 1nM activin A (R&D Systems, Minneapolis, MN) in serum-free DMEM with 0.1% BSA.
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bioRxiv - Cell Biology 2020Quote: ... and chondrogenic differentiation experiments were performed following the instructions of the human mesenchymal stem cell functional identification kit (R&D systems, SC006). For osteogenic differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...