Labshake search
Citations for R&D Systems :
2701 - 2750 of 2798 citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Filters were blocked in 5% nonfat milk in TBS-Tween 20 (0.1%) and incubated overnight with rabbit anti-NrCAM (R&D Systems AF8538, 1:1000), mouse anti-Rac1 (BD Biosciences # 610651 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
-
bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
-
bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
-
bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
-
bioRxiv - Bioengineering 2020Quote: ... medium was removed and the scaffolds designated for study in normal growth media were placed in wells with fresh normal growth medium and the mineralized collagen-AM scaffolds designated for study in inflammatory conditions were placed in wells with inflammatory medium (1 ng/ml of IL-1β recombinant porcine protein (R&D Systems, Minneapolis, MN) added to normal growth media) ...
-
bioRxiv - Bioengineering 2019Quote: hESCs were differentiated to SC-β cells as previously described9 by culturing in the following conditions in order: Stage 1: 3 d with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
-
bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
-
bioRxiv - Cell Biology 2021Quote: ... directly after UV irradiation or pre-treated 1 hour before irradiation with proteasome inhibitor MG132 (Enzo, 50 µM) or NEDD8 E1 Activating Enzyme Inhibitor (NAEi) MLN4924 (R&D systems, 10 µM) where indicated ...
-
bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
-
bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
-
Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
-
bioRxiv - Neuroscience 2022Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
-
bioRxiv - Microbiology 2023Quote: ... Huiskonen (University of Oxford) and incubated for three days at 37 °C in the presence of 1 μg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half a volume of binding buffer (25 mM HEPES ...
-
bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
-
bioRxiv - Molecular Biology 2024Quote: ... CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN). SEMA3C was measured using the human SEMA3C ELISA kit by LS Bio (LS Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
-
RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
-
bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
-
bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
-
bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
-
bioRxiv - Genomics 2024Quote: FACS-sorted Vcam1-FAPs were grown to 60-80% confluence followed by adipogenic differentiation and were treated with 500 ng/mL recombinant mouse sFRP-1 protein (R&D systems, 9019-SF) with or without sFRP-1 inhibitor ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
-
bioRxiv - Developmental Biology 2024Quote: ... Agents were added to the medium at final concentrations of 1 μg/mL for BMP4 treatment (314-BP; R&D Systems, Minneapolis, MN, USA), 1% v/v of 4 mM HCl containing 0.1% bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
-
bioRxiv - Genomics 2020Quote: ... LDLR staining was performed for 30 min on ice with a 1:50 dilution of AlexaFluor488-conjugated LDLR antibody (R&D Systems, Minneapolois MN, FAB2148G) into PBS supplemented with 1% FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... and buffers were prepared using the DuoSet ELISA Ancillary Reagent Kit 1 for the TGF-β and mTGF-β ELISAs (R&D Systems, Minneapolis, MN). Standards ...
-
bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
-
bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
-
bioRxiv - Cancer Biology 2020Quote: ... CHLA9 and CHLA10 cells were cultured in IMDM +L-glutamine media supplemented with 20% FBS and 1% insulin-selenium-transferrin (ITS, R&D Systems, cat no: AR013). Cell lines were maintained at 37°C and 5% CO2 ...
-
bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
-
bioRxiv - Neuroscience 2019Quote: ... half of the culture media was replaced with media alone or media containing recombinant mouse netrin-1 (R&D systems cat. no.1109-N1/CF), recombinant human ephrin-A5-Fc (R&D systems cat ...
-
bioRxiv - Developmental Biology 2019Quote: ... blocked (5 % BSA and 0.1 % Tween 20 in 1X TBS) for at least 1 hour at room temperature and stained with primary antibodies (Phospho-STAT3 Y705 and STAT3, R&D Systems 9131S and 9132S) diluted in blocking solution overnight at 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
-
bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were infected with HIV-1 (50 ng HIV-p24/106 cells) and then cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA) for up to 9 days ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
-
bioRxiv - Developmental Biology 2019Quote: Western blotting was performed as previously described (Chang et al., 2016, Mariani et al., 2016, Bay et al., 2018) with the following antibodies: Gli2 (R&D Systems, AF3635, 1:500), Gli3 (R&D Systems ...
-
bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
-
bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
-
bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
-
bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...