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Citations for R&D Systems :
2351 - 2400 of 2798 citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at 4°C and the supernatant was precipitated with antibodies against BACH1 (AF5776, R&D System), or IgG (normal mouse IgG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed and incubated with an HRP conjugated rabbit anti-mouse antibody (1:5000 diluted, R&D systems) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: CH12 cells were cultured for 72h in the presence of TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... anti-CD28 (1 µg/ml, clone CD28.2, eBioscience) and immobilized recombinant ICAM1-Fc fusion protein (0.5µg/ml, R&D Systems) in complete RPMI/HS medium ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were treated with the indicated neuropilin 1 ligands Semaphorin 3A (3nM; Cat#5926-S3-025, R&D Systems), VEGF-A165 (1nM ...
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bioRxiv - Neuroscience 2022Quote: Dissociated commissural neurons were stimulated with 0.1 µg/ml BSA or Netrin-1 (R&D Systems, # 1109-N1-025) for 5 or 30 min and fixed with 4% PFA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Gradients were generated in the Dunn chamber with 200 ng/ml Netrin-1 (R&D Systems, # 1109-N1-025) in the outer well ...
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bioRxiv - Biophysics 2022Quote: ... the gels were washed twice with 1xPBS and incubated at room temperature with a drop of 25μL of 0.01mg·mL-1 histidine- tagged E-cadherin (8505-EC-050, R&D Systems) covered with rectangles of parafilm ...
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bioRxiv - Synthetic Biology 2022Quote: ... membranes were probed with both an anti–6x-His tag antibody (R&D Systems, Cat # MAB050; diluted 1:7,500) and anti–ETEC O148 antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and incubated with an anti-mouse HRP-conjugated secondary antibody (R&D Systems; 1:1000) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Neuroscience 2022Quote: ... Then 1 ml of fresh media was supplemented with IL-34 (R&D systems, cat. No. 5265-IL-010), MCSF (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The neural medium was supplemented with epidermal growth factor (EGF, 20 ng ml-1, R&D Systems, 236-EG) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated to streptavidin (R&D Systems), diluted 40× in T-PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Human vasculature labeling was performed by using a cocktail of goat anti-podocalyxin (1:20, AF1658, R&D Systems), mouse anti-CD31 (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were washed in PBS and coated with 1 µg / mL laminin-I (R&D Systems; #3446-005-01) at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were pelleted and then resuspended in 1 mL of Flow Cytometry Staining Buffer 1X (R&D Systems #FC001) for blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Bioengineering 2023Quote: ... The primary antibodies used in this study include anti-Mouse Myosin Heavy Chain (1:1000, R&D Systems, MAB4470), anti-Human/Mouse/Rat/Chicken Pax7 (5μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: Western Blotting primary antibodies and working dilutions used were: goat anti-CD276 (1 µg/ml; R&D systems, #AF1027); mouse anti-α-tubulin (1:1’000 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used at the indicated dilutions: goat anti-Nurr1 (AF2156, R&D Systems; 1:250), rabbit anti-Nr2f2 (ab211776 ...
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bioRxiv - Neuroscience 2023Quote: ... Specified primary antibodies were incubated over-night at 4°C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31 ...
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bioRxiv - Neuroscience 2023Quote: ... The neural medium was supplemented with 20 ng ml−1 epidermal growth factor (EGF; R&D Systems, 236-EG) and 20 ng ml−1 basic fibroblast growth factor (FGF2 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for immunostaining of mice tissues: goat anti-CD31 (1:500; R&D systems), goat anti-LYVE1 (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... IL-1β release was measured using the Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit (R&D Systems) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... followed by matching horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature (R&D Systems, MN, USA). Chemiluminescent imaging was performed using the LAS-4000 platform and histogram densitometry was performed using Multi Gauge software (V3.1 Fujifilm ...
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated in permeabilization buffer containing the primary REG3β antibody (1:250 dilution; R&D systems, Minneapolis, MN) overnight (16 h) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies for western blot assays were used at the following concentrations: anti-SP-D (1:2,000, R&D Systems, AF1920), anti-LPO (1:2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... At day 1 the medium was changed to base media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Immunology 2022Quote: ... PE-conjugated anti-CTLA-4 and APC-conjugated anti-CD279 (PD-1) were purchased from R&D System (Minneapolis, MN). Human cytokines from cell culture supernatants were analyzed using ELISA kits for TNF ...
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bioRxiv - Neuroscience 2019Quote: Human TNFα was quantified in blood serum (1:10 dilution) using a DuoSet ELISA kit (R&D Systems, Minneapolis, MN). Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Bioengineering 2019Quote: ... we mixed laminin at 50 µg/mL with 50 µg/mL recombinant nidogen-1 (R&D systems 2570-Nd-050). Samples were immunostained as described below.
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Physiology 2021Quote: ... CCL2 levels in urine specimens were quantified using Human CCL2/MCP-1 Quantikine® ELISA Kit (R&D systems, DCP00) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... For proximity ligation assays VE-cadherin was detected using mouse VE-cadherin antibody (1:200, R&D systems, Goat, AF1002). c-Src was detected using anti-Src (GD11 clone ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-1 receptor antagonist (IL-1Ra) were measured in thawed supernatants using commercial ELISA kits (R&D Systems). IL-1Ra was measured because it inhibits the binding and bioactivity of IL-1β85 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems, UK). Plates were washed and 100 ul of substrate solution ...