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Citations for R&D Systems :
2651 - 2700 of 2795 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... The levels of adipokines in the plasma of WT and BaKO mice were measured using the Mouse Adipokine Array Kit (ARY013; R&D Systems) and LEGENDplex (740929 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then washed with FACS buffer and labeled with 1 µl/well of phycoerythrin-conjugated anti-mouse secondary (F0102B, R&D Systems) 1 hour at 4 °C ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of CXCL1 or CXCL2 protein in the supernatant was determined using a mouse CXCL1 or CXCL2 specific ELISA kit (R&D system). All experiments were performed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... An equivalent amount of CM (1.0 ml for each condition) was incubated with each nitrocellulose membrane provided in the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems). Membranes were developed following the instructions of the manufacturer.
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bioRxiv - Bioengineering 2023Quote: ... labeled with Alexa Fluor 488, Cat. #IC7619G) and anti-E-Cad (E- cadherin, Cat. #MAB18382, from mouse) were obtained from R&D systems. Anti-Vim (Vimentin ...
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bioRxiv - Cell Biology 2024Quote: ... overexpressed with or without V5-mEHMT1 and pretreated with or without palmitate and UNC0642 were quantified for 38 different adipokines using Proteome Profiler Mouse Adipokine Array Kit ARY013 from R&D Systems as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cultures were exposed for the duration of treatment with either 100ng/mL recombinant mouse Sfrp2 (R&D Systems Cat 1169-FR-025) or vehicle control (PBS with 0.1% BSA).
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Naïve T cells were isolated by negative selection using a Mouse Naive T Cell CD4+/CD62L+/CD44low Column Kit (R&D Systems). Naïve CD4 T cells were treated with fluorescein isothiocyanate-labeled (FITC ...
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bioRxiv - Physiology 2024Quote: ... 118.62 ng/ml; human, 50 ng/ml), and TNFα 1000U (mouse, 3.7 ng/ml; human, 13.15 ng/ml) (R&D Systems, Minneapolis, MN) or vehicle (0.1% PBSA ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... and tubulin were detected by immunoblotting using HIV-1 gag p24 antibody (1:1000 dilution, MAB7360, mouse monoclonal, R&D systems, Minneapolis, MN), human CD207 antibody (1:1000 dilution ...
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bioRxiv - Immunology 2021Quote: ... The cytokines released from macrophages treated with hemin were detected by mouse cytokine proteome profiler array panel A kit (ARY006, R&D Systems, USA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The TNF-alpha levels in the primary microglial supernatants were quantified using Quantikine® Mouse TNF-alpha ELISA kit (R&D Systems, MTA000B), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 100 µl of supernatant samples and standards (recombinant mouse TREM2-FC, 1729-T2; R&D Systems; 0-20 ng/ml in BSA-PBST) were then incubated for 2 hr at RT ...
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bioRxiv - Bioengineering 2020Quote: Cytokine and chemokine levels were measured in the basal outflow of mouse intestine chips using a custom MSD U-plex Assay (Meso Scale Diognostics) and the mouse CXCL1/KC DuoSet ELISA (R&D Systems DY453) according to the manufacturers’ instructions.
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Cell Biology 2021Quote: ... intracellular staining was done using the permeabilization buffer in the FIX & PERM kit with PE-conjugated anti-mouse α-SMA (clone: 1A4, R&D Systems) at 1:20 in the permeabilization buffer ...
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bioRxiv - Microbiology 2020Quote: ... and visualized on a Wes system (ProteinSimple, San Jose, CA, USA) with an anti-mouse or anti-goat IgG-HRP antibody (R&D systems, HAF109) detecting the primary antibody against GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2x10 minutes washes with milliQ water and 2x10 minutes washes with TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... IGF-1 was measured in the samples using a R&D Systems Mouse 1-Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD) ...
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bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was immediately used to determine the levels of IL1β and IL6 using Quantikine Mouse ELISA kits and following the manufacturer’s protocols (R&D Systems, Minneapolis, Mn).
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Immunology 2020Quote: ... except for the STORM experiments where they were used at a concentration of 20 μg/ml: α-MerTK (mouse extracellular monoclonal, 125618, R&D Systems), α-MerTK (goat extracellular polyclonal ...
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bioRxiv - Immunology 2021Quote: ... Each pooled sample was assayed for 40 different cytokines using the Proteome Profiler Mouse Cytokine Array Panel A kit according to manufacturer’s instructions (R&D Systems, Minneapolis, MN). Membranes were imaged using a LI-COR Odyssey Fc chemiluminescence imager (Lincoln ...
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bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647, clone H398, lot 0410; or APC-labelled anti-mouse gp130, R&D Systems, cat #FAB4681A ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNγ released into the supernatant by CAR-T cells was then measured using the DuoSet ELISA kit for mouse IFNγ (R&D systems, DY485) and Nunc MaxiSorp flat bottom plates (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
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bioRxiv - Immunology 2024Quote: IFN-γ protein levels were measured from cryopreserved neuron-CD8+ T cell co-culture supernatant using the Mouse IFN-γ DuoSet ELISA kit from R&D Systems (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... Protein lysates were subjected to enzyme-linked immunosorbent assay (ELISA) using mouse DuoSet® ELISA commercial kits (R&D Systems, Minneapolis, MN, USA), following manufacturer instructions.
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bioRxiv - Cancer Biology 2024Quote: ... A time course was performed on cells treated with 300 ng/mL of recombinant mouse VEGF-B186 (R&D Systems #767-VE-010) for 0 ...
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bioRxiv - Immunology 2023Quote: ... Plasma C-reactive protein levels were assessed by a Mouse C-Reactive Protein/CRP Quantikine ELISA Kit (R&D Systems, Inc., MN, USA) according to the manufacturer’s instructions.