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Citations for R&D Systems :
2351 - 2400 of 2795 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and immunostained with 0.5 μg/ml mouse monoclonal anti-MHC antibody (MF20; R&D Systems, Minneapolis, MN, USA). As a secondary anti-body ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human ACE2 protein levels were quantified from lung homogenates by ELISA (R&D Systems #DY3437, #DY933).
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bioRxiv - Genetics 2023Quote: ... The blots were then placed in primary antibody Polyclonal Goat IgG anti-mouse OSMR (R&D systems AF662) diluted in fresh block at 1:2500 and incubated on an orbital shaker at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies (Rabbit anti-MAP2, Millipore, AB5622, 1:800; Mouse anti-Tuj1, R&D Systems, mab1195, 1:500) were diluted in PBS and added to the wells to incubate overnight at 4°C while gently rocking ...
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bioRxiv - Immunology 2023Quote: Multiple human and mouse cytokines were analysed using Proteome Profiler Antibody Arrays (R&D Systems, ARY005 and ARY006). The kit membranes were incubated with culture media from human fibroblasts and mouse astrocytes infected with SeV or mock infected in accordance with the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Surface and intracellular antibodies used were against the following mouse antigens: CCL5 purified (Goat IgG, R&D Systems), CD107a BV786 (1D4B ...
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bioRxiv - Cancer Biology 2023Quote: ... ELISA assay was performed using a commercially available Quantikine Mouse TGFβ1 ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were incubated with 0.25 μg/ml biotinylated sheep anti-mouse TREM2 antibody (R&D Systems, BAF1729) for 1 hr at RT with shaking ...
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bioRxiv - Pathology 2023Quote: ... the isolated cells were subsequently enriched using an anti-mouse podoplanin (Pdpn) biotinylated antibody (BAF3244, R&D Systems) as above ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media supernatant was extracted and processed with the Mouse VEGF DuoSet ELISA (R&D Systems, DY493-05) according to manufacturer instructions.
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with anti-mouse protein C polyclonal sheep IgG (R&D Systems, Minneapolis, MN) and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... capillaries were filled with 200 ng/mL mouse integrin αvβ3 (7889-AV-050, R&D Systems, Abingdon, UK) and incubated at room temperature for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems); PE/Cyanine 7 anti-mouse CD325 (Ep-CAM ...
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Physiology 2023Quote: ... briefly centrifuged and stored at −80 °C before measurement (Mouse VEGF Quantikine ELISA Kit, R&D Systems, USA). Islets were collected and lysed in 50 μl T-PER buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Serum was assessed for presence of TNFα and MCP-1 using mouse ELISA kits (R&D SYSTEMS, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Adiponectin serum concentrations were quantified using the Quantikine ELISA Mouse Adiponectin/Acrp30 (Catalog: MRP30) from R&D Systems in accordance with the manufacturer’s guidelines after a 1:2000 serum dilution.
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bioRxiv - Physiology 2024Quote: ... Plasma GDF-15 was measured using the Mouse/Rat GDF-15 Quantikine ELISA Kit (R&D Systems, MGD150).
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2024Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Physiology 2024Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA ...
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bioRxiv - Pathology 2019Quote: ... FGF21 and leptin levels were measured through a Mouse Magnetic Luminex Screening Assay (R&D System, Minneapolis, MN, USA).
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bioRxiv - Physiology 2020Quote: Plamatic IgE level was evaluated by SDS PAGE using rat anti-mouse IgE antibodies (R&D systems Cat# MAB9935).