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Citations for R&D Systems :
2551 - 2600 of 2977 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... α-club cell secretory protein rat monoclonal antibody (MAB4218) was from R&D Systems (Minneapolis, Minnesota, USA) and the α-Muc5B rabbit polyclonal antibody (HPA008246 ...
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bioRxiv - Immunology 2021Quote: ... Cells were sequentially challenged by addition of the following agonist solutions: SLIGRL (100 μM; R&D Systems), then either rmIL-31 (300 nM ...
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bioRxiv - Cancer Biology 2021Quote: We cultured 3*105 million freshly enriched DCs in 2ml Dendritic Cell Base Media (R&D systems) plus 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... the endoderm cells were cultured in SFD medium supplemented with 1.5 μM dorsomorphin dihydrochloride (R&D Systems) and 10 μM SB431542 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... The cell lines were tested for mycoplasma contamination using MycoAlert® Mycoplasma Detection kit (R&D Systems) and if necessary cultured in the presence of Plasmocin™ treatment or Plasmocin™ prophylactic (InvivoGen) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were fed with either growth media or StemXVivo osteogenic media (R&D Systems, #CCM007 and CCM009). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... cell-free supernatants were collected and then assayed for murine IL-1 β (MLB00C, R&D Systems) by ELISA kit (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... cAMP levels were measured from cell lysates using the cAMP Parameter Assay Kit (R&D systems, KGE002B).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were treated for 24 h before processing according to the manufacturer’s protocol (ARY003C, R&D Systems). This phospho-kinase array contains capture antibodies to measure relative levels of phosphorylation of 37 kinases on a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with recombinant mouse VEGF-A (300 ng/mL, R&D Systems #493-MV-005), recombinant mouse VEGF-B186 (300 ng/mL) ...
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bioRxiv - Pathology 2024Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Treg were differentiated from the purified CD4+ cells using CellXVivo Mouse Treg differentiation kit (R&D System). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Cell death inducers in this study included rhTNF-α (210-TA/CF; R&D Systems, Minneapolis MN) and Staurosporine (9953S ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were confirmed to be mycoplasma negative using the MycoProbe kit (R&D Systems #CUL001B).
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bioRxiv - Microbiology 2023Quote: ... Uninfected cells were treated with DPBS or 1000U/ml of Universal Type 1 Interferon (R&D Systems) for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stimulated overnight with 5 pg/ml of BMP9 in serum-free medium (R&D Systems) and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then serum starved for 16 hours and VEGFC (R&D Systems, 9199-VC-025/CF) stimulation was performed for 0 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged and counted prior to lysis according to the HIF-1α ELISA (R&D Systems, the Human/Mouse Total HIF-1 alpha/HIF1α DuoSet IC ELISA Cat# DYC1935E ...
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bioRxiv - Developmental Biology 2023Quote: ... transfected cells were provided with 5 ng recombinant mouse TGFβ1 protein (R&D Systems, 7666-MB-005). mCherry fluorescence was determined first by exciting at 570 nm and measuring emission at 610 nm with a 100 msec time delay using a Perkin-Elmer EnVision Xcite Multilabel Plate Reader ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1) antibody (R&D Systems, AF3628,1:500), and rabbit anti-glial-fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2023Quote: Alkaline comet assays were performed using the CometAssay single cell gel electrophoresis assay kit (R&D systems) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8+ T cells were isolated from OTI mice by magnetic selection (R&D Systems, Cat# MAGM207) labeled with cell trace CFSE (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... cells were stimulated or not with 100 ng/ml mouse IL-17 recombinant protein (R&D Systems) for 10 or 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with either DMSO or 5 µM Idasanutlin (R&D Systems, #12-35-22-07) for an additional 24 h before cells were harvested for protein and RNA extraction.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were authenticated using a colorimetric signal amplification system and tested for mycoplasma contamination (R&D systems). All media were supplemented with penicillin and streptomycin (Gibco).
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bioRxiv - Bioengineering 2024Quote: ... The FL5.12 cell culture media was also supplemented with 100 ng/ml IL-3 (R&D Systems). L1210 cells were obtained from ATCC ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were embedded in Cultrex® RGF BME Type 2 (cat no. 3533-005-02, R&D systems) and placed in a 37°C incubator for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were treated with 100ng/ml Activin A (Shenandoah, 800-01) and 2um CHIR99021 (R&D Systems; 4423) for 24hrs ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were incubated with monoclonal anti-neuron-specific beta-III tubulin (Tuj-1, MAB1195 R&D Systems), rabbit polyclonal astrocyte-specific anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured as tumorspheres in Neurobasal medium supplemented with 10ng/mL EGF (R&D Systems, Minneapolis MN), 10ng/mL bFGF (R&D Systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell death was determined using TACS annexin V-FITC apoptosis detection kit (R&D systems, #4830-01-K) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were cultured in R10 media containing 5 ng/mL IL-7 (R&D systems). C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies ...
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bioRxiv - Immunology 2022Quote: ... Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... For cell treatment the following inhibitors and growth factors were used: FGF (R&D Systems, 233-FB-025), EGF (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cells were blocked with 1% BSA and stained overnight with goat anti-ACE2 (1:200, R&D Systems) in blocking buffer followed by rabbit anti-goat Alexa Fluor 488 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... For 8 oxo-dG we performed ICC on PFA fixed cells with a specific antibody (R&D System) to quantify DNA oxidation ...
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bioRxiv - Bioengineering 2022Quote: ... 90 μl of cells were incubated with 10 μl of anti-FAP-PE (clone 427819, R&D Systems) for 20 minutes at room temperature before washing with FACS buffer ...
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bioRxiv - Cell Biology 2019Quote: Co-localization experiments were performed on HUVE cells seeded on fibronectin-coated (5 μg/mL, R&D Systems) No ...
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bioRxiv - Pathology 2021Quote: ... 1 million cells per well were plated in 12-well Cultrex coated (R&D Systems, 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with directly-conjugated LILRB3 mAb (clone 222821; mouse IgG2a; R&D Systems, UK) for 20 minutes at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were maintained in proliferating state using 10 ng/ml fibroblast growth factor 2 (FGF2; R&D Systems) and passaged twice before usage in the experiments ...
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bioRxiv - Neuroscience 2021Quote: PGE2 in the media of MSC and differentiated cells was determined by a PGE2 immunoassay (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... cells were differentiated for 14 days with or without Ihh supplement (150ng/ml, R&D Systems Europe LTD). The cells were harvested for RNA isolation on day 0 ...
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bioRxiv - Immunology 2020Quote: IL-1β and CXCL9 were measured using ELISA (IL-1β from cell culture supernatants: MLB00C, R&D Systems; IL-1β from serum ...
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bioRxiv - Microbiology 2021Quote: ... cells were maintained in SFD media supplemented with the following: 3 μM CHIR 99021 (CHIR, R&D Systems), 10 ng/ml human FGF10 ...
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bioRxiv - Immunology 2020Quote: ... To detect IL-22 cells were cultured overnight with 40ng/ml recombinant mouse IL-23 (R&D Systems). To prepare γδ T cells and CD4+ T cells for cell sorting ...
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bioRxiv - Immunology 2020Quote: ... Selected cells were then induced to undergo CSR with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... CD14+ cells were differentiated with 20 ng/mL of macrophage colony-stimulating factor (M-CSF) (R&D systems) for 3 days followed by 3 days of polarization into naïve macrophages (M0 ...
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bioRxiv - Immunology 2021Quote: ... while Th17 cell cultures were supplemented with IL-6 (20 ng/mL; R&D Systems 206-IL-010), TGF-β (10 ng/mL ...