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Citations for R&D Systems :
2501 - 2550 of 2644 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... DIV12 hippocampal neurons were treated with either 2 ug/mL of recombinant Nptx2 (CF 7816-NP-050, R&D Systems) or BSA as control ...
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Immunology 2023Quote: ... mouse TNF-alpha DuoSet ELISA (DY410) and DuoSet ELISA Ancillary Reagent Kit 2 (DY008) were purchased from R&D system.
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bioRxiv - Cell Biology 2023Quote: ... A feeding layer of 2 ml RPMI growth medium supplemented with 10 ng/ml PDGF-BB ligand (R&D Systems) and/or 0.01 uM siRNA (see below ...
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tissue pellet was resuspended in cold Cultrex Reduced Growth Factor BME type 2 (Cultrex BME) (R&D systems) and 25 mL drops per well were plated on pre-warmed 48-well plates ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed three times with PBST and incubated with 15 μl of DAB (3,3’-Diaminobenzidine) chromogen (2 ml of DAB chromogen (860001, R&D Systems) diluted in DAB chromogen buffer (860005 ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Developmental Biology 2023Quote: ... media was changed daily with hindgut medium (RPMI 1640, 1X GlutaMAX, 2% defined FBS, 2µM CHIR99021 and 500ng/ml FGF4 (R&D Systems) until day 8 ...
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were encapsulated in 50% Cultrex basement membrane extract type 2 (BME-II) (R&D Systems; catalog #3533-010-02) and maintained in advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Isolated naive CD4+ T cells were resuspended in isolation media supplemented with recombinant IL-2 (R&D Systems, #202-IL-050) at 10 ng/mL (growth media ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded on laminin-521 coated plates at a split ratio of 1:2.5 in CPN medium supplemented with 20 ng/mL fibroblast growth factor 2 (FGF2; R&D Systems) and 10 µM Y-27632 ...
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bioRxiv - Cancer Biology 2021Quote: ... culture media were changed to serum free media or serum free media with 2 ug/ml IGF2 antibody (AF-292, R&D Systems) for another 72 hours ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Bioengineering 2020Quote: Injured animals were randomly selected to receive intralesional injections of eCS matrix functionalized with the neurotrophic factors fibroblast growth factor (FGF-2; 50μg/mL; R&D Systems; 233FB025CF) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... fragments were washed three times in 5 ml cold AdDF+++ before being embedded in 30 μL BME (Type 2; R&D Systems) per well in a 48-well plate ...
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bioRxiv - Cancer Biology 2021Quote: Slit2 concentrations in mice serum were determined by the sandwich ELISA method with the DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the coverslips were transferred to a 48-well plate and cultured in Neurobasal-A medium + 2% B27 + 50 ng/ml of neurturin (R&D Systems) + 50 ng/ml of neuronal growth factor (R&D Systems ...