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Citations for R&D Systems :
2351 - 2400 of 2644 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Th0 cell cultures were provided IL-2 (15 U/mL; R&D Systems 202-IL-500), while Th17 cell cultures were supplemented with IL-6 (20 ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 ng/ml glial cell-derived neurotrophic factor (GDNF, R&D Systems, Minneapolis, MN, USA). The experiments were conducted on flasks or multi-well plates (Nunc ...
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bioRxiv - Neuroscience 2024Quote: ... or 2 μg of mouse anti-ERRγ antibody (R&D Systems #PP-H6812-00, one replicate) in CUT&RUN Wash buffer for 10min with rotation at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Then plated into Matrigel domes (Cultrex PathClear reduced growth factor BME, type 2; R&D Systems) in a 24 well plate ...
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bioRxiv - Immunology 2023Quote: ... Lipocalin-2 (NGAL) was assessed with a Quantikine ELISA Kit (Biotechne R&D Systems, Minneapolis MN). Additionally ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then incubated with 2 μg of anti-galectin-9 (AF2545, R&D systems) or isotype control under rotation ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-rabbit Nucleocapsid (NovusBiologics ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-mouse H3 (Immune-Tech ...
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bioRxiv - Immunology 2024Quote: ... in the presence or absence of rhIL-15 or rhIL-2 (R&D Systems, Minneapolis, MN) and the indicated antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... AOs were dissolved away from Cultrex® RGM Type 2 (R&D systems, #3533-010-02) and fixed in 4 % paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Neuroscience 2019Quote: The secretion of cytokines in AD TREM2 neuronal cultures before and after stimulation with the Aß-S8C dimer was measured employing the Proteome Profiler Human Cytokine Array kit (R&D System, United States of America). The assay was performed following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IP-10 and TGF-β levels were measured by enzyme-linked immunosorbent assay (ELISA) sandwich using human CXCL-10/IP-10 DuoSet ELISA (R&D Systems Inc, MN, USA) and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-γ concentrations in cell-free supernatants were determined by Human IFN-γ DuoSet enzyme-linked immunosorbent assay (ELISA; DY285; R&D systems, Minneapolis, MN, USA). EYFP−/EBFP+ dLP-CHO cells with no anti-hPD1 payload circuit were used as the control cells.
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bioRxiv - Cell Biology 2022Quote: The presence of angiogenic factors in BEC CM was evaluated trough a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D Systems Inc., Minneapolis, MN, USA). For this ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 3 units/mL EPO (Amgen, EPOGEN NDC 55513-144-01), 200 μg/mL holo-transferrin (Millipore Sigma, T0665, 10 ng/mL recombinant human SCF (R&D systems, 255-SC/CF), and 1 ng/mL recombinant human interleukin IL-3 (R&D systems ...
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bioRxiv - Immunology 2024Quote: ... cells were maintained in resting conditions by culturing in simple R10 or R10 supplemented with 10 ng/mL of recombinant human interleukin-7 (IL-7, R&D Systems, cat. BT-007-010) for one week.
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Physiology 2019Quote: ... tissue from the wound area was harvested and stored at −80°C for analysis of VEGF using an enzyme-linked immunosorbent assay (murine VEGF-specific ELISA kit (R&D Systems, USA) and a BCA protein assay kit (Thermo scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Immunology 2021Quote: All cells were grown in a humidified incubator at 37°C and 5% CO2 and cell lines tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems).
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked in 3% bovine serum albumin for 30 min and incubated with the following primary antibodies overnight at 4°C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... the conditioned media was stored at -80° C and the interleukin-6 (IL-6) content was quantified via enzyme-linked immunosorbent assay (R&D Systems, DY406) following the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant of BALF was store at -80°C for cytokine analysis by mouse TNF-α and IL-1β DuoSet ELISA kits (R&D Systems). Lungs were perfused with a 1:1 mixture of OCT and 4% PFA and preserved in OCT cryomolds (Tissue-Tek ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...