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Citations for R&D Systems :
201 - 250 of 3888 citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 400 µg of protein from mock- and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Cell Biology 2022Quote: ... NED] and 3U.ml−1 EPO [Roche]) supplemented with 1 ng.ml−1 IL→ 3 and 10 ng.ml−1 SCF (both R&D Systems) and 1 µg.ml−1 doxycycline for 4 days ...
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bioRxiv - Developmental Biology 2022Quote: ... Jagged 1 (AF599, R&D systems, 1:500), Dlk1 (AF1144 ...
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bioRxiv - Physiology 2023Quote: ... VCAM-1 (AF643, 1:1000, R&D Systems), BNP (ab19645 ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems) in tissue culture flasks ...
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bioRxiv - Biophysics 2019Quote: ... and Fc-ICAM-1 or Fc-VCAM-1 (Intercellular Adhesion Molecule 1; Vascular Cell Adhesion Molecule 1, R&D system, Minneapolis, MN) solution at 10 μg/mL overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... goat-α-neuorpilin-1 (1:400, R&D Systems), mouse-α-ROBO2(1:50 ...
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bioRxiv - Neuroscience 2022Quote: ... Nestin (1:2000–1:5000, R&D Systems, MAB1259), Pax6 (1:300 ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... 1 µM dorsomorphin homolog 1 (4126, R&D Systems), 3 µM CHIR99021 (13122 ...
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bioRxiv - Immunology 2023Quote: ... anti-mLamp-1 (1:1000, AF4320, R&D Systems), anti-Phospho-p38 (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... Parvalbumin (R&D system AF5058, 1:50 – 1:100), cFOS (Santa Cuz s-166940 ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Spermine-NONOate was used at 50mM ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Images were acquired in DIC every 15 sec by using a Prime95B camera (Photometrics) ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:200; R&D Systems AF5857). Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:300; R&D Systems AF5857), anti-Flag (Mouse HRP ...
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bioRxiv - Microbiology 2021Quote: ... FIZZ-1/RELM (1:1000, Ref. AF1523, R&D Systems), pCREB (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... LYVE-1 (1:100, AF2089, R&D Systems, Minnesota, USA) CD32B (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... TuJ-1 monoclonal (R&D systems, Cat# MAB1195, 1:500); Alexa Fluor 568 conjugated phalloidin (Thermo Fischer Scientific ...
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bioRxiv - Genomics 2023Quote: ... 1% normal goat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a wet chamber with the appropriate antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... intercellular adhesion molecule 1 (ICAM-1) (R&D systems, DY796), and E-selectin (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... LOX-1 (clone 214012, 1:50) from R&D Systems; Siglec-E (clone M1304A01 ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4C overnight ...
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bioRxiv - Pathology 2024Quote: ... anti-mouse Mac-1 (R&D Systems, dilution 1:100) or anti-mouse CD3 (Abcam ...
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bioRxiv - Immunology 2023Quote: ... 1% normal rat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a humid chamber with the appropriate antibodies ...