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Citations for R&D Systems :
2251 - 2300 of 2902 citations for IL 9 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Biochemistry 2023Quote: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of IL-6 (DY206) and VEGFA (DY293B-05) in the conditioned media was measured by using ELISA DuoSet Kit (R&D Systems, Minneapolis, MN, USA). The concentration was calculated by following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ splenic T-lymphocytes were polarized to IL-17A-producing phenotypes ex vivo by use of CellXVivo Mouse TH17 Cell Differentiation Kit (#CDK017, R&D Systems, Minneapolis, MN, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Immunology 2019Quote: ... Levels of IL-1β and TNF-α in 200 μl culture medium were measured by ELISA kits according to manufacturer’s instructions (DY401 for IL-1 β and DY410 for TNF-α, R&D systems, Minneapolis, MN, USA). Cleavage of pro-IL-1β and pro-caspase-1 was assessed by western blot of 50 μg of protein extracts of cell lysates ...
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bioRxiv - Immunology 2022Quote: ... NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL rhIL-2 (R&D Systems, Cat. 202-IL-010). NK cells were then placed into a 37°C incubator ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Immunology 2019Quote: We measured the concentrations of IL-1β and tumor necrosis factor alpha (TNF-α) in culture supernatants by using a commercial ELISA kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2024Quote: ... TNF-α and IL-6 release was measured using DuoSet ELISA kits according to the manufacturer’s instructions (R&D Systems: DY417-05; DY410-05; DY406-05).
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bioRxiv - Bioengineering 2023Quote: The conditioned media from treated RAW264.7s was used to quantify levels of multiple secreted cytokines/chemokines using their respective ELISA kits including IL-6 (DY406; R&D Systems Incorporated, Minneapolis, MN, USA), TNF-⍰ (DY410 ...
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bioRxiv - Cell Biology 2020Quote: ... we used a proteome profiler for human phospho-kinases (ARY003C, R&D Systems, Biotechne, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... L-glutamine (2mM) and 50 ng/mL of recombinant human M-CSF (R&D Systems, Minneapolis, Minnesota) for 6 to 7 days to differentiate them to macrophages [41] ...
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bioRxiv - Molecular Biology 2021Quote: ... purified 6xHis-Clr4 protein was mixed with human Ubiquitin-activating Enzyme (UBE1, R&D systems E-305), human UbcH5c/UBE2D3 (R&D systems E2-627) ...
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bioRxiv - Immunology 2019Quote: ... Following transfection cells were stimulated with 200 U/ml of recombinant human (h) TNFα (R&D Systems). Luciferase activity was assayed in cell lysates harvested 8 h post-stimulation ...
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bioRxiv - Cancer Biology 2019Quote: ... the phospho-MAPK array assay was performed using the human phospho-MAPK array kit (R&D Systems) according to the provided protocol.
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bioRxiv - Physiology 2019Quote: ... fully differentiated mouse or human brown adipocytes were incubated with 100ng/ml activin B (R&D Systems) in DMEM/F12 supplemented with 10% FBS and 100µg/ml penicillin-streptomycin for 24h before lysis and for RNA extraction of RNA (as indicated below) ...
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bioRxiv - Cancer Biology 2019Quote: Apoptosis array was performed by using Proteome Profiler Human Apoptosis Array Kit (ARY009) from R&D Systems following manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... The concentration of OPN was determined by the Human Osteopontin (OPN) DuoSet ELISA (DY1433, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used to immunostain human tissue: PDGFRβ (1:100, goat, R&D Systems), GFAP (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells were stained with Goat anti-Human Phycoerythrin-conjugated ACE2 Polyclonal Antibody (R&D Systems) for 30 min at 4 °C in dark ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human Fms-related tyrosine kinase 3 ligand (Flt3L, #308-FK, all R&D Systems), 2 mM L-GlutaMAX (Gibco #35050-038) ...
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bioRxiv - Cancer Biology 2020Quote: Cytokine profiling was performed simultaneously using the Human XL Cytokine Array (R&D Systems, ARY022, Minneapolis, MN). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... GDF-15 expression was analyzed using Human GDF-15 DuoSet® ELISA (DY957; R&D Systems™). For the activation of latent TGF-β1 ...
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bioRxiv - Cell Biology 2020Quote: ... USA) and human recombinant lumican protein (lacking GAG chains) was from R&D Systems (Minneapolis, MN, USA). CS sodium salt isolated from bovine cartilage and ChABC from Proteus vulgaris were from Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human sFRP-1 and antihuman Dkk-1 antibodies (cat# CCM017; R&D Systems; Minneapolis, MN, USA) for 15 days ...
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bioRxiv - Cancer Biology 2020Quote: SHP2 phosphatase activity was determined using the human/mouse/rat active DuoSet IC kit (R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were incubated overnight with nitrocellulose membranes of the Human Phospho-Kinase Array (R&D Systems). Membranes were then washed ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatants were collected and assayed in a Proteome Profiler Human Chemokine Array (ARY017, R&D systems) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and stained overnight at 4°C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled with the following antibodies: anti-human/mouse GFRA2 Polyclonal Goat IgG (R&D Systems), rat monoclonal anti-PDGFR beta antibody conjugated with PE (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... cells were treated with different concentrations of recombinant human IFN-β (R&D Systems, 8499-IF-010) for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... we used a Proteome Profiler™ Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA) which contained 102 different capture antibodies that were spotted on a nitrocellulose membrane ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor® 647-conjugated anti-human lymphatic vessel endothelial receptor-1 (LYVE-1 ,R&D Systems) or Alexa Fluor™ 488-conjugated anti-human laminin (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... TNFα was measured in human monocyte-derived macrophage culture supernatants by ELISA (#DY210-05, R&D systems). All cytokine detection was performed according to manufacturer’s instructions.
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... Levels of IL6 and TNFα were assessed with a murine and human ELISA kit (R&D Systems) respectively ...
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bioRxiv - Microbiology 2022Quote: ... Plasma LBP was measured by quantitative ELISA using human LBP DuoSet kits (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were assayed immediately after using the Quantikine® Human TGF-β1 Immunosasay Kit (R&D Systems), the Human TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Systems Biology 2020Quote: ... with 10% Fetal Bovine Serum and 100ng/ml recombinant Human M-CSF (R&D Systems; 216-MC) for 5 days ...
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bioRxiv - Pathology 2020Quote: Human platelet lysate was immunoprecipitated using IgG or anti-cadherin-6 antibody (R&D Systems, catalog # MAB2715) and Protein A beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Conditioned culture media were analyzed using the Proteome Profiler™ Human XL Cytokine Array (R&D Systems). Signals were visualized with enhanced chemiluminescence on a Bio-Rad ChemiDoc™ MP Imaging system ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated for 2 h with human E-cadherin-Fc (R&D system, Minneapolis, MN) at 1 μg / cm2 ...
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bioRxiv - Immunology 2019Quote: ... Cytokine production was quantified in the supernatants by Human XL Cytokine Array kit (ARY022B; R&D Systems), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... A polyclonal antibody against human ACE2 and anti-IDE polyclonal antibody were purchased from R&D Systems (catalog No ...