Labshake search
Citations for R&D Systems :
2501 - 2550 of 2902 citations for IL 9 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 104 sorted CD34+ HSPCs were plated for methylcellulose-based colony forming assays [Human Methylcellulose Base Medium HSC002 (R&D Systems), which were supplemented with 2% streptomycin/penicillin ...
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bioRxiv - Microbiology 2021Quote: ... ELISA was performed using Human Gas6 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, Minnesota, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemokine levels were measured using the Invitrogen ELISA Kit (Quantikine mouse/human CXCL10/IP-10; R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... with mouse and human soluble FLT1-FC chimeric protein (471-F1-100 and 321-FL-050/CF, R&D Systems) immobilized to about 1,000 RU was used as ligand for the analyte binding of anti-FLT1 MAbs ...
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human WNT3A (#5036-WN-010) and WNT4 (#6076-WN-005) were obtained from R&D Systems (Minneapolis, MN, USA) and reconstituted per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... the concentration of amphiregulin in the supernatant was determined using the Human Amphiregulin Quantikine ELISA Kit (R&D Systems, DAR00) according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2020Quote: Plasma VEGF-A concentration was measured using the quantitative sandwich ELISA kit (Human VEGF Quantikine; R&D Systems, Minneapolis, MN) following the manufacturer’s instructions in our laboratory at low altitude (600 m) ...
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bioRxiv - Immunology 2022Quote: ... immune mediators in cell supernatants were measured using Human Custom 29-plex Multi-Analyte Kit (R&D Systems, Minneapolis, Minnesota) measuring the following analytes ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Biochemistry 2024Quote: The human cell line H1299 was acquired from ATCC and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Immunology 2024Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: For Wnt5a experiments tetracycline induced GFP-RasV12 cells were treated with 100ng/mL recombinant human/mouse Wnt5a (R&D Systems) or PBS (control-Sigma-Aldrich ...
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bioRxiv - Pathology 2023Quote: CCL2 was quantified in conditioned media from HAoSMCs using Human CCL2/MCP-1 Quantikine ELISA Kit (DCP00, R&D systems) according to the manufacture’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Neuroscience 2023Quote: Levels of soluble VEGF-A were assessed by a Human VEGF-A Quantikine ELISA kit (Bio-techne R&D Systems), according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... MDMs were stimulated in complete RPMI with either 10 ng/mL human IFN-ψ (285-IF-100, R&D Systems) or 50 ng/mL LPS ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were plated into semi-solid methylcellulose (MethoCult™ H4435 Enriched, STEMCELL Technologies or Enriched Human Methylcellulose, R&D Systems), with further addition of 100 pg/ml rh-IL21 ...
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bioRxiv - Immunology 2023Quote: Phosphoprotein expression of CD8+ T cells was detected using a human phospho-kinase array kit (ARY003C, R&D Systems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: The levels of INFγ were evaluated in supernatants obtained from 0.625:1 and 1.25:1 effector:target ratio overnight co-cultures of CAR T-cells:CAFs using the Human IFN-Gamma Quantikine Kit (R&D Systems) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiation was induced by addition of human recombinant Activin A (ActA, 30 ng/ml, R&D systems, 338-AC-050).
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and florescent antibody for 15 minutes on ice: PB2 PE (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Immunology 2023Quote: ... Production of immune mediators in the supernatants was assessed using a human 45-plex (R&D Systems, Inc. Minneapolis, Minnesota).
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with complete osteogenic medium mixed by human/mouse StemXVivo osteogenic/adipogenic base medium (CCM007, R&D Systems) and mouse StemXVivo ostegenic supplement (CCM009 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of angiogenic proteins in CM was measured using a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Cell Biology 2023Quote: ... GPNMB levels in the supernatant were measured by ELISA (DuoSet ELISA Development system, Human Osteoactivin/GPNMB, #DY2550, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... IFN-β amounts in the culture supernatant were measured by the human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... Tissue constructs were cultured in the presence of 10ng/ml TGF-β3 (human recombinant active TGF-β3, R&D Systems) for the initial 14 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 cells stably expressing the human D1R (D1-HEK) were split into poly-L-lysine (R&D systems, Minneapolis, MN) coated 6-well plates at 450,000 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... each 25μl of pelleted resin was incubated with 2μg of recombinant human K63-linked (R&D systems, Cat# UC-320) or K48-linked (R&D Systems ...
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bioRxiv - Systems Biology 2019Quote: ... Sera were subjected to depletion of abundant serum proteins using Proteome Purify 12 Human Serum Protein Immunodepletion Resin (R&D Systems). Denaturing was followed by alkylation with N-ethylmaleimide and acetone precipitation ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were collected and cytokine concentrations measured using CXCL10/IP10 (Human CXCL10/IP-10 Quantikine ELISA Kit, R&D Systems) and IFN-β (VeriKine-HS Human IFN Beta Serum ELISA Kit Cat No 41415 ...
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bioRxiv - Immunology 2019Quote: ... Soluble CD14 (sCD14) concentration in plasma samples was measured using the human CD14 Quantikine ELISA kit from R&D systems (DC140) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... was added to 2mL of fresh differentiation media and either 50 ng/µl of human IFNγ 285-IF (R&D systems) was added or not ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... or the indicated compounds were added at 12.5µM and incubated for 30 min at room temperature prior to adding biotinylated human VEGF-A165 (Cat#BT293, R&D systems) at 10 nM ...