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Citations for R&D Systems :
2101 - 2150 of 2242 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 2fTGH and HEK293T cells and ELISA was performed according to the manufacturer’s protocols for human IFN-β (R&D systems DY814-05PBL Assay Science 41410-2), human IFN-γ (Biolegend 43104 ...
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bioRxiv - Genomics 2024Quote: ... 3 units/mL EPO (Amgen, EPOGEN NDC 55513-144-01), 200 μg/mL holo-transferrin (Millipore Sigma, T0665, 10 ng/mL recombinant human SCF (R&D systems, 255-SC/CF), and 1 ng/mL recombinant human interleukin IL-3 (R&D systems ...
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bioRxiv - Immunology 2024Quote: ... cells were maintained in resting conditions by culturing in simple R10 or R10 supplemented with 10 ng/mL of recombinant human interleukin-7 (IL-7, R&D Systems, cat. BT-007-010) for one week.
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (R&D Systems, Minneapolis, USA), and separately in combination with IL-4 and IL-21 (100 IU each) ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF10A cells were treated for 3 days with 5 ng/ml of TGF-β1 (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Physiology 2023Quote: ... The supernatants were diluted when necessary to be in the optimal linear optical range and analyzed using commercial kits for IFNγ (Human IFN-gamma DuoSet ELISA, R&D Systems, USA and Bio legend, UK), and IL-10 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: Islet MTs were cultured for 1 day (short-term) or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... The chamber was prepared by adding 26μl pre-warmed (37°C) CXCL-5 (0, 0.5, 50, 500ng/ml) or CCL2 (0, 0.5, 50, 500ng/ml) (R&D systems) in DMEM to the lower chamber ...
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bioRxiv - Microbiology 2021Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minnesota, MN). For total and differential cell counts ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Bioengineering 2019Quote: ... 0.25 ng/mL BMP4 (PeptroTech 315-27) and 5 ng/mL Activin A (R&D Systems 338-AC-050/CF) were added to the SF differentiation medium ...
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bioRxiv - Immunology 2020Quote: ... two group of 5 mice each were treated with monoclonal anti-mouse IL-25 (mα-IL-25) (R&D Systems) or monoclonal anti-mouse IL-4Rα (mα-IL-4Rα ...
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bioRxiv - Microbiology 2023Quote: Cytokine analyses were performed on the 5 mL of collected BALF via DuoSet ELISA kits (R&D Systems, Minneapolis, MN). For total and differential cell counts ...
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bioRxiv - Physiology 2023Quote: ... intra-assay % coefficient of variation [%CV] 5%-2.5%), alanine transaminase (ALT) (Siemens Healthcare, intra-assay %CV 8.1%-3.6%) and growth differentiation factor 15 (GDF15) (R&D Systems, BioTechne ...
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bioRxiv - Immunology 2022Quote: ... were washed and resuspended in PBS with 0.02% Tween-20 (PBS-T) and loaded with recombinant human IL13Rα2-Fc chimera (R&D Systems Cat#614INS; 2.1 µg per 3.9×107 beads) by incubation for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... SKBr3 cells co-transfected with EGFP-α-tubulin and the indicated siRNA or cDNA were grown on collagen-coated glass coverslips for 48 h and observed 10 min after the addition of 5 nM HRGβ1 (R&D Systems) using the 63X objective (plan Apochromat NA 1.4 ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and cardiac fibroblasts were serum starved for 24 h before adding medium containing 5 ng/ml recombinant TGFβ1 for 2 days (R&D Systems, Minneapolis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 min at 4°C) pre-incubated with 5 μl TRKB antibody (Goat anti-mouse TRKB Antibody, #AF1494, R&D Systems) and cleared through new Protein-G sepharose tubes ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Microbiology 2019Quote: ... using known concentrations of commercial rBoIFN-γ (20, 15, 10, 5, 3 and 1 ng; 2300-BG-025, R&D Systems).
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/mL FGF1 and 5 μg/mL heparin or 10 ng/mL PDGF-AA (R&D Systems #1055-AA-050) supplemented DMEM for 12 hrs.
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bioRxiv - Developmental Biology 2020Quote: ... Micromass cultures were then maintained at 37°C in a humidified incubator with 5% CO2 for 24hrs with the inhibitors before treatment with 5ng TGFβ1/ml (R&D Systems) for the indicated times.
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bioRxiv - Neuroscience 2020Quote: ... OPCs were treated with LCN2 (1, 2.5, 5, 10, 20, 40, 80, or 160 ng/ml, R&D Systems, Shanghai, China) or vehicle control (0.0002% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with increasing doses of TGFβ2 (vehicle control, 1.25 ng/ml, 2.5 ng/ml, and 5 ng/ml; R&D Systems, Minneapolis, MN) for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...